Surface-MALDI mass spectrometry in biomaterials research

Griesser, Hans J.; Kingshott, Peter; McArthur, Sally L.; McLean, Keith M.; Kinsel, Gary R.; Timmons, Richard B.

doi:10.1016/j.biomaterials.2004.01.049

Cited by 70 publications

(76 citation statements)

References 56 publications

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“…1 -3 MALDI-TOF achieves very low mass detection limit in the attomole to nanomole range. Therefore, it has a high sensitivity with an accuracy of 10 3 -10 4 . 4 MALDI-TOF has a large mass range that makes it ideal for biomaterials characterization of lipids, proteins, polymers and surface analysis.…”

Section: Introductionmentioning

confidence: 98%

“…Therefore, it has a high sensitivity with an accuracy of 10 3 -10 4 . 4 MALDI-TOF has a large mass range that makes it ideal for biomaterials characterization of lipids, proteins, polymers and surface analysis. However, it has been difficult to use MALDI-TOF for the analysis of low-mass molecules and considerable efforts have been made to overcome this problem.…”

Section: Introductionmentioning

confidence: 98%

“…8,35,36 As a result of the non-destructive nature of the technique, the bonding between molecules in the film is intact and the reproducibility between samples is good. 1,2,4,8 Octadecylphosphonic acid (ODPA) was used to form SAMs on titanium, iron and stainless steel. Furthermore, carboxyalkylphosphonic acid was used to form organic molecules on titanium as reported.…”

Section: Introductionmentioning

confidence: 99%

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An approach to differentiating between multi‐ and monolayers using MALDI‐TOF MS

Quiñones

Raman

Gawalt

2007

Surface & Interface Analysis

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Analysis and confirmation of monolayer film thickness on metal oxide surfaces has proven to be challenging. XPS and AFM have been used to investigate the monolayer formation. However, these techniques are difficult to access and/or determine the composition of the organic molecules on the surfaces. Here we demonstrate the ability of MALDI-TOF to characterize long alkyl chain phosphonic acid molecules in thin films on titanium, iron and stainless steel. These systems are known to be stable, strongly adhered films. The thin films were characterized by IR, AFM, contact angle measurements and the results were confirmed by MALDI-TOF. Moreover, the MALDI-TOF was used to differentiate between mono-and multilayers on planar surfaces.

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Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

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An approach to differentiating between multi‐ and monolayers using MALDI‐TOF MS

Quiñones

Raman

Gawalt

2007

Surface & Interface Analysis

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“…This method is widely used in studies of biofilms, e.g. adsorption of proteins/peptides from biofluids (tears) [9]. One study investigating the efficacy of the peptide adsorption and desorption during the MALDI ionization process provided evidence for the difficult desorption of peptides from surfaces with high binding affinities to the molecules studied [8].…”

Section: Introductionmentioning

confidence: 99%

“…In the past 10 years, the development of innovative MALDI probes such as patterned hydrophobic and ionic layers on gold surface [4][5][6] or polymeric supports [7][8][9] has proven to be a viable alternative to previous traditional methods (e.g. solid-phase extraction systems, microdialysis, or AnchorChips ™ ) of sample preparation and measurement.…”

Section: Introductionmentioning

confidence: 99%

Atmospheric molding of ionic copolymer MALDI‐TOF/MS arrays: A new tool for protein identification/profiling

Muck¹,

Ibáñez²,

Stauber³

et al. 2006

Electrophoresis

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An atmospheric molding protocol has been used to prepare an ionic methacrylate-based copolymer sample support chips for MALDI (pMALDI)-MS by targeting selected groups of various monomers copolymerized during molding, namely, carboxy, sulfo, dimethylalkyamino, and trimethylalkylammonium groups. The new disposable array chips provide analyte-oriented enhancement of protein adsorption to the modified substrates without requiring complicated surface coating or derivatization. The MALDI-MS performance of the new ionic copolymer chips was evaluated for lysozyme, beta-lactoglobulin A, trypsinogen and carbonic anhydrase I using washing with solutions prepared in pH or ionic strength steps. On cationic chips, the proteins are washed out at pH lower than their p/ values, and on anionic chips at pH higher than their p/ values. The ability of the microfabricated pMALDI chip set to selectively adsorb different proteins from real samples and to significantly increase their MS-signal was documented for the transmembrane photosystem I protein complex from the green alga Chlamydomonas reinhardtii. The proteins were almost exclusively adsorbed according to calculated pI values and grand average of hydropathy (GRAVY) indexes. The new disposable chips reduce manipulation times and increase measurement sensitivity for real-world proteomic samples. The simple atmospheric molding procedure enables additional proteomic operations to be incorporated on disposable MALDI-MS integrated platforms.

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Synthesis and studies of blue light emitting polymers containing triphenylamine‐substituted fluorene and diphenylanthracene moiety

Barve

Raut

Mishra

et al. 2011

J of Applied Polymer Sci

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Photoluminescent (PL) polymers containing triphenylamine-substituted fluorene and diphenylanthracene (DPA) units were synthesized by aromatic nucleophilic substitution reaction. The light emitting polymers (LEPs) contains hole-transporting triphenylamine (TPA) groups at the C-9 position of fluorene and DPA-emitting segments in the main chain. The obtained polymers were soluble in various organic solvents and thermally stable. The synthesized polymers were successfully characterized by elemental analyses, FTIR and, 1 H NMR spectroscopy. The electrochemical measurements and optical properties of the polymers were also studied. The obtained polymers showed significant blue emission.

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Surface-MALDI mass spectrometry in biomaterials research

Cited by 70 publications

References 56 publications

An approach to differentiating between multi‐ and monolayers using MALDI‐TOF MS

An approach to differentiating between multi‐ and monolayers using MALDI‐TOF MS

Atmospheric molding of ionic copolymer MALDI‐TOF/MS arrays: A new tool for protein identification/profiling

Synthesis and studies of blue light emitting polymers containing triphenylamine‐substituted fluorene and diphenylanthracene moiety

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