ABSTRACT:Highland environments favor accumulation and preservation of soil organic matter (SOM) due to low temperatures, leading to the formation of Histosols. The Itatiaia National Park (INP), Rio de Janeiro, Brazil, offers conditions for preservation of SOM deposited over time, which has led to the formation of these soils. The objective of this study was to characterize Histosols in this environment, with the premise that it may provide evidence of changes in vegetation. Organossolos (Histosols) were sampled, characterized, described, and analyzed for their properties, stable isotopes of 12 C and 13 C, 14 N and 15 N, and dating of organic matter through 14 C. The soils were classified in the Brazilian Soil Classification System as Organossolo Háplico Hêmico típico -RJ-01 (Haplohemists) and Organossolo Fólico Sáprico cambissólico -RJ-02 (Udifolists). The morphological properties, degree of transformation, and chemical fractioning of SOM were consistent with hemic and sapric materials. The δ 13 C and δ 15 N isotopic analyses showed a difference in the contribution of plant materials. In RJ-01, there was previous influence of algae, due to poor drainage, and depletion of δ 13 C, suggesting a mix of C 3 and C 4 plants. RJ-02 showed influence of C 3 type plants. The 14 C dating for RJ-01 was 3280±80 years, and for RJ-02, 2005±5 years (modern age).