Surface Markers for Chondrogenic Determination: A Highlight of Synovium-Derived Stem Cells

Campbell, Douglas D; Pei, Ming

doi:10.3390/cells1041107

Cited by 30 publications

(19 citation statements)

References 63 publications

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“…Many studies have shown that oxygen deprivation helps in chondrogenic differentiation and maturation (Choi et al 2014b;Merceron et al 2010;Weijers et al 2011), but the beneficial effect of serum deprivation on chondrogenic differentiation is reported for the first time. It has been reported that high expression of CD90, CD73 and CD 105 are associated with positive enhancement in chondrogenic differentiation and maturation of MSCs (Arufe et al 2010;Campbell and Pei 2012;Jiang et al 2010). Our findings show that there is no significant (p \ 0.05) difference in terms of expression of those markers in ASCs cultured in all conditions including those cultured in 21 % O 2 without serum, suggesting that chondrogenic differentiation enhancement in serum deprivation condition may be affected by other factors.…”

Section: Discussionmentioning

confidence: 45%

The effects of hypoxia and serum-free conditions on the stemness properties of human adipose-derived stem cells

et al. 2016

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The need to have a better and safer culture condition for expansion of human mesenchymal stem cells (MSCs) is crucial particularly to prevent infection and immune rejection. This is normally associated with the use of animal-based serum in the culture media for cell expansion. The aim of this study is to investigate alternative culture conditions which may provide better and safer environment for cell growth. In the present study, human adipose-derived stem cells (ASCs) at passage 3 were subjected to treatment in 4 conditions: (1) 21 % O2 with fetal bovine serum (FBS), (2) 21 % O2 without FBS, (3) 2 % O2 with FBS and (4) 2 % O2 without FBS followed by subsequent analysis of their phenotype, viability and functionality. We observed that ASCs cultured in all conditions present no significant phenotypic changes. It was found that ASCs cultured in 2 % O2 without serum showed an increase in viability and growth to a certain extent when compared to those cultured in 21 % O2 without serum. However, ASCs cultured in 2 % O2 without serum displayed a relatively low adipogenic and osteogenic potential. On the other hand, interestingly, there was a positive enhancement in chondrogenic differentiation of ASCs cultured in 21 % O2 without serum. Our findings suggest that different culture conditions may be suitable for different indications. In summary, ASCs cultured in serum-free condition can still survive, proliferate and undergo subsequent adipogenic, osteogenic and chondrogenic differentiation. Therefore, FBS is feasible to be excluded for culture of ASCs, which avoids clinical complications.

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Section: Discussionmentioning

confidence: 45%

The effects of hypoxia and serum-free conditions on the stemness properties of human adipose-derived stem cells

et al. 2016

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“…(13) In joint tissue, Thy-1 is also expressed by synovial fibroblast, chondrocyte, and mesenchymal progenitor/stem cells. (14) The precise biological function of Thy-1 in joint tissue is not clear. It has been reported that the expression of Thy-1 in chondrocyte was correlated with its chondrogenic potential.…”

Section: Discussionmentioning

confidence: 99%

Characterization of Monoclonal Antibodies Against a Human Chondrocyte Surface Antigen

Chanmee

Phothacharoen

Thongboonkerd

et al. 2013

Monoclonal Antibodies in Immunodiagnosis and Immunotherapy

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Chondrocytes express a number of cell-surface molecules that mediate cell-cell or cell-matrix interactions. Identification and full characterization of new chondrocyte surface molecules will lead to a better understanding of the function of the chondrocyte. Researchers used primary human chondrocytes as an immunogen, and various monoclonal antibodies (MAbs) were generated using standard hybridoma technology. A monoclonal antibody named 5D2 was selected for further characterization. The antigen recognized by 5D2 MAb is expressed by primary human chondrocytes, primary synovial fibroblasts, synovial fibroblast cell lines (SW982), primary skin fibroblasts, and osteoblasts, but not expressed in blood cells. Biochemical analysis revealed that the 5D2 antigen is a protein with a molecular weight of approximately 25-35 kDa. Protein identification by mass spectrometry and molecular cloning revealed that 5D2 antigen is identical to the Thy-1 molecule. Furthermore we confirmed this specificity of the antibody by the isolated and cloned Thy-1 gene to the COS-7 and probed it with the 5D2 antibody using Western blot analysis. We examined the role of the Thy-1 molecule in arthritis models and tissue; one was papain-induced rat arthritis, the other was immunohistological staining of osteoarthritic (OA) human articular cartilage. OA cartilage showed a higher expression of Thy-1 as compared with normal tissue in all experimental approaches. The in vitro studies showed that the inflammatory cytokine interleukin-1β up-regulated Thy-1 molecule expression in the cartilage tissue. It can be concluded that the Thy-1 might be a potential biomarker for cartilage pathogenesis, degradation, and metabolic turnover.

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“…In contrast to the CD73 + subpopulation, a high percentage of these CD271 + cells were also positive for CD105. Their findings suggest that there may be more complexity in the relationships between different surface markers in the differentiation capacity of subpopulations, rather than perhaps any one individual marker [72]. Other potential surface markers related to chondrogenesis that have been reported in the literature include CD166 [73][74][75], CD90 [76,77], CD29 [66], and CD39 [78].…”

Section: Arufe Et Al Found That the Cd73mentioning

confidence: 99%

Heterogeneity of Progenitor Cell Populations and their Therapeutic Implications

Zielins¹,

Luan²,

Tevlin³

et al. 2014

J Stem Cell Res Ther

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Surface Markers for Chondrogenic Determination: A Highlight of Synovium-Derived Stem Cells

Cited by 30 publications

References 63 publications

The effects of hypoxia and serum-free conditions on the stemness properties of human adipose-derived stem cells

The effects of hypoxia and serum-free conditions on the stemness properties of human adipose-derived stem cells

Characterization of Monoclonal Antibodies Against a Human Chondrocyte Surface Antigen

Heterogeneity of Progenitor Cell Populations and their Therapeutic Implications

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