Surface mass spectrometry of molecular species

Hanley, Luke; Kornienko, Oleg; Ada, Earl T.; Fuoco, Erick; Trevor, Jennifer L.

doi:10.1002/(sici)1096-9888(199907)34:7<705::aid-jms845>3.0.co;2-b

Cited by 77 publications

(123 citation statements)

References 114 publications

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“…We are currently examining mass spectrometric methods that will enable more detailed chemical characterization. [17] These methods should allow, for example, the determination of whether the peptides have been modified following covalent attachment to the silicone surface.…”

Section: Recent Advances and Future Workmentioning

confidence: 99%

Peptides Bound to Silicone Membranes and 3D Microfabrication for Cardiac Cell Culture

et al. 2002

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Mechanical load and stress are exerted upon heart muscle tissue in vivo, leading to changes in cellular biochemistry and physiology. Understanding the fundamental mechanisms of mechanical transduction at the cellular level requires the development of physiologically relevant models of cells in vitro. Silicone elastomers are widely used as flexible cell culture substrates for in‐vitro cardiac mechano‐biological studies. We describe here the use of peptide binding to and microfabrication of silicone elastomer membranes. We utilize these chemically and morphologically modified silicone membranes as improved flexible cell culture substrates in studies of cardiac mechano‐biology. We also discuss advances in surface modification and microfabrication that promise further development of improved heart cell cultures.

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Section: Recent Advances and Future Workmentioning

confidence: 99%

Peptides Bound to Silicone Membranes and 3D Microfabrication for Cardiac Cell Culture

et al. 2002

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“…The analysis of FFPE tissue opens hospital sample banks, which represent an enormous amount of well documented tissues, whereas fresh samples are sometimes more scarce. Other sample preparation and instrumental strategies include, e.g., targeting chemical families with functionalized nanoparticles and development of post-ionization sources [25][26][27][28].…”

mentioning

confidence: 99%

On-tissue protein identification and imaging by MALDI-Ion mobility mass spectrometry

Stauber

MacAleese

Franck

et al. 2010

J. Am. Soc. Mass Spectrom.

188

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MALDI imaging mass spectrometry (MALDI-IMS) has become a powerful tool for the detection and localization of drugs, proteins, and lipids on-tissue. Nevertheless, this approach can only perform identification of low mass molecules as lipids, pharmaceuticals, and peptides. In this article, a combination of approaches for the detection and imaging of proteins and their identification directly on-tissue is described after tryptic digestion. Enzymatic digestion protocols for different kinds of tissues-formalin fixed paraffin embedded (FFPE) and frozen tissues-are combined with MALDI-ion mobility mass spectrometry (IM-MS). This combination enables localization and identification of proteins via their related digested peptides. In a number of cases, ion mobility separates isobaric ions that cannot be identified by conventional MALDI time-of-flight (TOF) mass spectrometry. The amount of detected peaks per measurement increases (versus conventional MALDI-TOF), which enables mass and time selected ion images and the identification of separated ions. These experiments demonstrate the feasibility of direct proteins identification by ion-mobility-TOF IMS from tissue. The tissue digestion combined with MALDI-IM-TOF-IMS approach allows a proteomics "bottom-up" strategy with different kinds of tissue samples, especially FFPE tissues conserved for a long time in hospital sample banks. The combination of IM with IMS marks the development of IMS approaches as real proteomic tools, which brings new perspectives to biological studies. (J Am Soc Mass Spectrom 2010, 21, 338 -347)

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“…This points to another issue in LDPI-MS:Å theÅ resultsÅ presentedÅ hereÅ reaffirmÅ [8]Å that strong linear optical absorption of an analyte can lead to enhanced desorption yields by resonant laser desorption.Å Finally,Å 7.87Å eVÅ SPIÅ canÅ alsoÅ beÅ utilizedÅ with other desorption probes, including the accelerated atomic or cluster primary ions used in secondary ion massÅ spectrometryÅ [12].…”

Section: Discussionmentioning

confidence: 99%

“…SPI has been observed for gaseous molecular species produced during laser desorption, evaporation, thermal desorption, and keV ion sputtering [6 -21]. Laser desorption combined with various laser postionization schemes have been studied for many years and is well established [8,9,12,[22][23][24], although not as popular as other desorption/ ionization schemes. However, the recent expansion in the use of commercial atmospheric pressure photoionization sources [17] indicates the promise of vacuum ultraviolet (VUV) photoionization in vacuum for mass spectrometric applications.…”

mentioning

confidence: 99%

7.87 eV laser desorption postionization mass spectrometry of adsorbed and covalently bound bisphenol a diglycidyl methacrylate

Zhou

Akhmetov

et al. 2007

J. Am. Soc. Mass Spectrom.

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Bisphenol A diglycidyl methacrylate (Bis-GMA) was adsorbed onto or covalently bound to a porous silicon oxide surface. Laser desorption 10.5 eV postionization mass spectrometry (LDPI-MS) was previously demonstrated for surface analysis of adsorbed and surface bound Bis-GMA, but signal to noise levels were low and ion fragmentation was extensive. 7.87 eV postionization using the fluorine laser was demonstrated here for Bis-GMA. However, signal levels remained low for LDPI-MS of Bis-GMA as its ionization potential (IP) was only ϳ7.8 eV, near threshold for single photon ionization by the 7.87 eV fluorine laser. It is known that aromatic tagging of molecular analytes can lower the overall IP of the tagged molecular complex, allowing 7.87 eV single photon ionization. Therefore, Bis-GMA was also derivatized with several tags whose IPs were either below or above 7.87 eV: the tag with an IP below 7.87 eV enhanced single photon ionization while the tags with higher IPs did not. However, signal intensities were enhanced by resonant laser desorption for two of the derivatized Bis-GMAs. Intact ions of Bis-GMA and its derivatives were generally observed by 7.87 eV LDPI-MS, consistent with the formation of ions with relatively little internal energy upon threshold single photon

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Surface mass spectrometry of molecular species

Cited by 77 publications

References 114 publications

Peptides Bound to Silicone Membranes and 3D Microfabrication for Cardiac Cell Culture

Peptides Bound to Silicone Membranes and 3D Microfabrication for Cardiac Cell Culture

On-tissue protein identification and imaging by MALDI-Ion mobility mass spectrometry

7.87 eV laser desorption postionization mass spectrometry of adsorbed and covalently bound bisphenol a diglycidyl methacrylate

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