Surface Modification by Nano-Structures Reduces Viable Bacterial Biofilm in Aerobic and Anaerobic Environments

Abstract

Bacterial biofilm formation on wet surfaces represents a significant problem in medicine and environmental sciences. One of the strategies to prevent or eliminate surface adhesion of organisms is surface modification and coating. However, the current coating technologies possess several drawbacks, including limited durability, low biocompatibility and high cost. Here, we present a simple antibacterial modification of titanium, mica and glass surfaces using self-assembling nano-structures. We have designed two different nano-structure coatings composed of fluorinated phenylalanine via the drop-cast coating technique. We investigated and characterized the modified surfaces by scanning electron microscopy, X-ray diffraction and wettability analyses. Exploiting the antimicrobial property of the nano-structures, we successfully hindered the viability of Streptococcus mutans and Enterococcus faecalis on the coated surfaces in both aerobic and anaerobic conditions. Notably, we found lower bacteria adherence to the coated surfaces and a reduction of 86–99% in the total metabolic activity of the bacteria. Our results emphasize the interplay between self-assembly and antimicrobial activity of small self-assembling molecules, thus highlighting a new approach of biofilm control for implementation in biomedicine and other fields.

Bifico relieves irritable bowel syndrome by regulating gut microbiota dysbiosis and inflammatory cytokines

“…Previous research evidenced that Lactobacillus acidophilus could produce bifidogenic growth factors to stimulate the growth of Bifidobacterium longum in pure culture [ 48 ] . Enterococcus faecalis could create anaerobic conditions, which might be of benefit for survival of Bifidobacterium [ 49 ]. In addition, Enterococcus spp.…”

Bifico Relieves Irritable Bowel Syndrome By Regulating Gut Microbiota Dysbiosis and Inflammatory Cytokines in an Animal Model

“…has been used as a probiotic to defend against gut infection and prevent the colonization of more pathogenic bacteria [39]. In addition, Enterococcus faecalis could create anaerobic conditions, which might be of bene t for survival of Bi dobacterium [40].…”

Peptide- and Metabolite-Based Hydrogels: Minimalistic Approach for the Identification and Characterization of Gelating Building Blocks

“…The peptide-based multi-responsive hydrogels with unique and improved properties using coarse-grained and atomistic simulations can be useful in exosome delivery, with an emphasis on bioadhesion, organoids, and tissue engineering [ 26 ]. The remarkable properties of peptide- and metabolite-based hydrogels suggest their potential use in 3D bioprinting, tissue engineering, antibacterial and wound-healing materials, drug delivery, anti-bacterial, tumor therapy, tissue engineering, water remediation, and other biomedical applications [ 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 ].…”