Surface modification of cyclic olefin copolymer substrate by oxygen plasma treatment

Hwang, Shug-June; Tseng, Mei-Chih; Shu, J.-R.; Yu, Hsin Her

doi:10.1016/j.surfcoat.2008.01.016

Cited by 79 publications

(88 citation statements)

References 22 publications

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“…Obviously, the density of such reactive groups can be easily varied by altering the plasma oxidation parameters [19]. We believe that this result could be very useful for fast attachement of biorecognition elements in experiments that do not require sophisticated surface treatment.…”

Section: Discussionmentioning

confidence: 95%

Functionalization of cyclo-olefin polymer substrates by plasma oxidation: Stable film containing carboxylic acid groups for capturing biorecognition elements

Gubala

Gandhiraman

et al. 2010

Colloids and Surfaces B: Biointerfaces

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Many current designs in biomedical diagnostics devices are based on the use of low cost, disposable, easyto-fabricate chips made of plastic material, typically a cyclo olefin polymer (COP). Low autofluorescence properties of such material, among others, make it ideal substrate for fluorescence based applications.Functionalization of this plastic substrate for biomolecule attachment is therefore of great importance and the quality of films produced on such surface have often a significant influence on the performance of the device. In this communication we discuss the surface chemistry and some other characteristics of hydrophilic films, containing carboxylic acid functional groups, formed by plasma oxidation of COP and also films containing cross linked, polymerized acryclic acid produced by sequential deposition of tetraorthosilicate and acrylic acid by Plasma Enhanced Chemical Vapor Deposition (PECVD).Immobilization of labeled, single stranded DNA revealed high binding capacity for both coatings. To our best knowledge, this is the first example of direct immobilization of biomolecules on just plasma oxidized COP. Furthermore, more sophisticated treatment of the oxidized plastic substrate by PECVD with other organic precursors increased the binding capacity by some 40% than that of just plasma oxidized COP. The carboxy functionalized surfaces, due to the negative charge of the carboxy groups, showed very positive trends towards increasing the signal to noise ratio when charged biomolecules such as DNA, are used.

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Section: Discussionmentioning

confidence: 95%

Functionalization of cyclo-olefin polymer substrates by plasma oxidation: Stable film containing carboxylic acid groups for capturing biorecognition elements

Gubala

Gandhiraman

et al. 2010

Colloids and Surfaces B: Biointerfaces

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“…It utilises the different evanescent field 20 penetration depth of light polarised perpendicular-and parallel-to the waveguide surface to derive thickness and refractive index information about the surface coatings. DPI has been verified using standard protein systems and has been demonstrated in the successful monitoring of biochemical 25 interactions, e.g., protein adsorption 16,17 and lipid membrane formation 18,19 . The specific purpose of these measurements was to investigate the structural changes caused to the film by washing with surfactant solutions.…”

Section: Dual Polarization Interferometrymentioning

confidence: 98%

“…Qualitatively, the C 1s spectra for all films (Figure 2 communications that deal with the changes in chemistry and morphology of COP substrates either just after plasma oxidation [22][23][24][25][26] or the subsequent depositions of alkylsiloxanes, such as APTES. In light of these reports we rationalize that it is not very likely that the polymerized siloxane films contain 25 C-O-C or -C-OH bonds. Therefore, the peak at 286.4 eV is best attributed to C-N bonding, corresponding to the terminal ÐCH 2 -NH 2 functionality.…”

Section: Qualitative Characterization By X-ray Photoelectron Spectrosmentioning

confidence: 99%

“…Information about the mass and the density of the adsorbed A30 and A4 films were derived, based on which the amount 25 of amino groups for both A30 and A4 samples was calculated, assuming that the silane does not fragment completely but retains its aminosiloxane molecular identity. Therefore, from the mass of the film per cm 2 and the molar mass of the monomer, the number of moles of ÐNH 2 in the film per cm 2 of 30 geometric area can be calculated.…”

Section: Coating Thickness and Swelling In Contact With Water: Dual Pmentioning

confidence: 99%

“…However, in order to fully exploit their potential, considerable research and 25 development is still necessary, especially regarding the substrate functionalization for biomolecule immobilization 1,2 .…”

mentioning

confidence: 99%

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Functionalization of cycloolefin polymer surfaces by plasma-enhanced chemical vapour deposition: comprehensive characterization and analysis of the contact surface and the bulk of aminosiloxane coatings

Gubala

Gandhiraman

Volcke

et al. 2010

Analyst

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Click‐and‐Bond: Room‐Temperature and Solvent‐Free Bonding of Polymer‐Based Microfluidic Devices

Iorio

Rameshbabu

Bruijns

et al. 2022

Adv Materials Inter

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The numerous advances in the field of microfluidics have enabled the successful implementation of microfluidic devices in (bio)sensing for a wide range of applications, including medical diagnostics. [3] In particular, the coupling of microfluidic devices with sensing modules or sample-pre-treatment modules have led to major advances in the realization of microfluidic devices for both point-of-care (POC) and portable usage. [4] Rigid thermoplastic polymers are nowadays widely explored as innovative materials for the fabrication of microfluidic devices, due to the cost-effective and high-volume production alternatives that they offer compared to the more traditionally used materials such as glass and silicon. [5][6][7] Polycarbonate, poly(methyl methacrylate), polystyrene, and cyclic olefin copolymer (COC) have emerged as particularly attractive substrate materials for this type of applications owing to their beneficial physico-chemical properties. Among several properties, their high transparency and low autofluorescence enable, for example, the use of widespread and advantageous optical techniques, such as fluorescence detection, for biosensing applications. [8] Current focus on personalized healthcare has sparked the expansion of the functionalities of microfluidic devices, particularly in the use of chemically specific coatings. Among the aforementioned innovative polymeric materials, COC has emerged as a suitable material for a large range of applications owing to multiple beneficial properties. Its good chemical resistance, biocompatibility, excellent transparency, and high glass transition temperature (T g ) represent the most interesting characteristics for the production of devices. [9] In particular, the high resistance of COC toward common solvents favors the use of such materials over the so far largely employed polydimethylsiloxane (PDMS)-based materials. [7,10] PDMS presents, in fact, a major disadvantage of swelling in contact with solvents such as chloroform, benzene, acetone, and ethanol. Additionally, surface chemical treatments of PDMS have been reported to be unstable over time. [11,12] However, the intrinsically hydrophobic nature of COC substrates promotes non-specific binding of biomolecules and prevents the flow of aqueous solutions through the channels. [13]

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Surface modification of cyclic olefin copolymer substrate by oxygen plasma treatment

Cited by 79 publications

References 22 publications

Functionalization of cyclo-olefin polymer substrates by plasma oxidation: Stable film containing carboxylic acid groups for capturing biorecognition elements

Functionalization of cyclo-olefin polymer substrates by plasma oxidation: Stable film containing carboxylic acid groups for capturing biorecognition elements

Functionalization of cycloolefin polymer surfaces by plasma-enhanced chemical vapour deposition: comprehensive characterization and analysis of the contact surface and the bulk of aminosiloxane coatings

Click‐and‐Bond: Room‐Temperature and Solvent‐Free Bonding of Polymer‐Based Microfluidic Devices

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