Surface modification of microparticles causes differential uptake responses in normal and tumoral human breast epithelial cells

Patiño, Tania; Soriano, Joan Manuel; Barrios, Leonardo; Ibáñez, Elena; Nogués, Carme

doi:10.1038/srep11371

Cited by 70 publications

(70 citation statements)

References 48 publications

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“…Non-CSFE-labeled NDMPs (Figure 5c,d, top) and CSFE-labeled NDMPs (Figure 5c,d, bottom) were co-incubated non-trypan blue-labeled (Figure 5c) and trypan-blue labeled (Figure 5d) peritoneal myeloid cells from naive mice for 1 hour. Treatment with CSFE-labeled NDMPs of quenched, trypan blue-labeled myeloid cells demonstrates persistent CSFE uptake, indicating NDMP endocytosis (46). Taken together, these data demonstrate that the majority of NDMPs express PS, which likely serves as an uptake signal to surrounding phagocytes, specifically F4/80 macrophages.…”

Section: Resultsmentioning

confidence: 99%

Neutrophil derived microparticles increase mortality and the counter-inflammatory response in a murine model of sepsis

Johnson

Prakash

Rice

et al. 2017

Biochimica et Biophysica Acta (BBA) - Molecular Basis of Diseas

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Although advances in medical care have significantly improved sepsis survival, sepsis remains the leading cause of death in the ICU. This is likely due to a lack of complete understanding of the pathophysiologic mechanisms that lead to dysfunctional immunity. Neutrophil derived microparticles (NDMPs) have been shown to be the predominant microparticle present at infectious and inflamed foci in human models, however their effect on the immune response to inflammation and infection is sepsis has not been fully elucidated. As NDMPs may be a potential diagnostic and therapeutic target, we sought to determine the impact NDMPs on the immune response to a murine polymicrobial sepsis. We found that peritoneal neutrophil numbers, bacterial loads, and NDMPs were increased in our abdominal sepsis model. When NDMPs were injected into septic mice, we observed increased bacterial load, decreased neutrophil recruitment, increased expression of IL-10 and worsened mortality. Furthermore, the NDMPs express phosphatidylserine and are ingested by F4/80 macrophages via a Tim-4 and MFG-E8 dependent mechanism. Finally, upon treatment, NDMPs decrease macrophage activation, increase IL-10 release and decrease macrophage numbers. Altogether, these data suggest that NDMPs enhance immune dysfunction in sepsis by blunting the function of neutrophils and macrophages, two key cell populations involved in the early immune response to infection.

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Section: Resultsmentioning

confidence: 99%

Neutrophil derived microparticles increase mortality and the counter-inflammatory response in a murine model of sepsis

Johnson

Prakash

Rice

et al. 2017

Biochimica et Biophysica Acta (BBA) - Molecular Basis of Diseas

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“…A strong increase in uptake efficiency, to 16%, was also observed for N7 whereas no significant influence of the lipofectamine coating was seen for mouse astrocytes and SH-SY5Y. We conclude that surface coating and increased incubation time or a combination thereof are suitable methods to achieve high tagging efficiencies in a variety of cell types, even though the intracellular recognition and processing of the resonators by the cells may differ between the two approaches21242526. (We did not find any indication that the different approaches for laser internalization affect the spectral properties of the lasers.…”

Section: Resultsmentioning

confidence: 75%

“…In the literature, different liposome coatings have been reported to assist with the uptake of much smaller polymer microspheres (about 2 μm in size) by non-phagocytic cells212324. Liposomes consist of a formulation of neutral and positively charged lipid molecules that can facilitate contact and fusion with negatively charged cell membranes.…”

Section: Resultsmentioning

confidence: 99%

Lasing in Live Mitotic and Non-Phagocytic Cells by Efficient Delivery of Microresonators

Schubert

Volckaert

Karl

et al. 2017

Sci Rep

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Reliable methods to individually track large numbers of cells in real time are urgently needed to advance our understanding of important biological processes like cancer metastasis, neuronal network development and wound healing. It has recently been suggested to introduce microscopic whispering gallery mode lasers into the cytoplasm of cells and to use their characteristic, size-dependent emission spectrum as optical barcode but so far there is no evidence that this approach is generally applicable. Here, we describe a method that drastically improves intracellular delivery of resonators for several cell types, including mitotic and non-phagocytic cells. In addition, we characterize the influence of resonator size on the spectral characteristics of the emitted laser light and identify an optimum size range that facilitates tagging and tracking of thousands of cells simultaneously. Finally, we observe that the microresonators remain internalized by cells during cell division, which enables tagging several generations of cells.

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“…It has been reported that MCF-10A cells can internalize both positively and negatively charged particles, whereas in SKBR-3 cells the uptake of negative charged particles is low [39,40]. The differences in cell uptake can be explained because Na-ZnTCPP-1·GNP are negatively charged.…”

Section: Photodynamic Effect Of Na-zntcpp-1·gnp On Cell Culturesmentioning

confidence: 94%

Amphiphilic gemini pyridinium-mediated incorporation of Zn(II)meso-tetrakis(4-carboxyphenyl)porphyrin into water-soluble gold nanoparticles for photodynamic therapy

Alea-Reyes

Soriano

Mora-Espí

et al. 2017

Colloids and Surfaces B: Biointerfaces

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Abstract:Zn-containing porphyrins are intensely investigated for their ability to form reactive oxygen species and thereby being potent photosensitizers for use in photodynamic therapy (PDT). Some of the drawbacks of the PDT approach, such as unspecific distribution, could be addressed by means of photosensitizer drug delivery systems. In this work, we synthesize and characterize new water-soluble gold nanoparticles (GNP) stabilized by a mixture of a polyethyleneglycol-containing thiol (to improve water solubility) and a new amphiphilic gemini-type pyridinium salt, which also acts as promotor of the incorporation of the anionic photosensitizer Na-ZnTCPP into the GNP.The obtained GNP have sizes between 7-10 nm, as observed by Transmission Electron Microscopy. The incorporation of the photosensitizer caused an increase in the hydrodynamic size, detected by Dynamic Light Scattering, as well as a shift in the Surface Plasmon Resonance peak on the GNP UV-visible absorption spectra. The presence of the photosensitizer in the GNP was corroborated using Fluorescence Spectroscopy. The amount of Na-ZnTCPP was found to be 327 molecules per GNP. The porphyrincontaining Na-ZnTCPP-1·GNP showed good enhanced ability to produce singlet oxygen, compared to free Na-ZnTCPP. Their cytotoxicity and phototoxicity were investigated in vitro using two different human breast cell lines, one of tumoral origin (SKBR-3) and another of normal epithelium origin (MCF-10A). SKBR-3 cells showed higher sensitivity to Na-ZnTCCP and Na-ZnTCPP-1·GNP in dark conditions. After irradiation, no significant differences were observed between both cell lines except for 1µM Na-ZnTCCP-1·GNP where SKBR-3 cells were also more sensitive.

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Surface modification of microparticles causes differential uptake responses in normal and tumoral human breast epithelial cells

Cited by 70 publications

References 48 publications

Neutrophil derived microparticles increase mortality and the counter-inflammatory response in a murine model of sepsis

Neutrophil derived microparticles increase mortality and the counter-inflammatory response in a murine model of sepsis

Lasing in Live Mitotic and Non-Phagocytic Cells by Efficient Delivery of Microresonators

Amphiphilic gemini pyridinium-mediated incorporation of Zn(II)meso-tetrakis(4-carboxyphenyl)porphyrin into water-soluble gold nanoparticles for photodynamic therapy

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