Surface modification of PDMS via self-organization of vinyl-terminated small molecules

Poll, Maaike L. van; Khodabakhsh, Saghar; Brewer, Paul J.; Shard, Alexander G.; Ramstedt, Madeleine; Huck, Wilhelm T. S.

doi:10.1039/b901763a

Cited by 32 publications

(28 citation statements)

References 43 publications

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“…This latter approach is valid in the case of large particles of 100 nm diameter or greater with shells of the order of atomic thickness. 22 The line shown on the graph results from an implementation of the T NP formula, 23 which was also the method employed by participants H, J, K, L, M, N and P indicated by square filled symbols (■). The T NP equation is provided in the Supplementary Information.…”

Section: Resultsmentioning

confidence: 99%

Versailles Project on Advanced Materials and Standards Interlaboratory Study on Measuring the Thickness and Chemistry of Nanoparticle Coatings Using XPS and LEIS

et al. 2016

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We report the results of a VAMAS (Versailles Project on Advanced Materials and Standards) inter-laboratory study on the measurement of the shell thickness and chemistry of nanoparticle coatings. Peptide-coated gold particles were supplied to laboratories in two forms: a colloidal suspension in pure water and; particles dried onto a silicon wafer. Participants prepared and analyzed these samples using either X-ray photoelectron spectroscopy (XPS) or low energy ion scattering (LEIS). Careful data analysis revealed some significant sources of discrepancy, particularly for XPS. Degradation during transportation, storage or sample preparation resulted in a variability in thickness of 53 %. The calculation method chosen by XPS participants contributed a variability of 67 %. However, variability of 12 % was achieved for the samples deposited using a single method and by choosing photoelectron peaks that were not adversely affected by instrumental transmission effects. The study identified a need for more consistency in instrumental transmission functions and relative sensitivity factors, since this contributed a variability of 33 %. The results from the LEIS participants were more consistent, with variability of less than 10 % in thickness and this is mostly due to a common method of data analysis. The calculation was performed using a model developed for uniform, flat films and some participants employed a correction factor to account for the sample geometry, which appears warranted based upon a simulation of LEIS data from one of the participants and comparison to the XPS results.

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Section: Resultsmentioning

confidence: 99%

Versailles Project on Advanced Materials and Standards Interlaboratory Study on Measuring the Thickness and Chemistry of Nanoparticle Coatings Using XPS and LEIS

et al. 2016

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“…The microchannel surface must be turned hydrophilic to assure an effective wetting of the channel walls by the continuous aqueous solution. The various surface modification methods 3,4 found in literature have significant drawbacks:…”

Section: Introductionmentioning

confidence: 99%

Hydrophilic PDMS microchannels for high-throughput formation of oil-in-water microdroplets and water-in-oil-in-water double emulsions

et al. 2010

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Here we present a novel surface modification method based on the sequential layer-by-layer deposition of polyelectrolytes yielding hydrophilic microchannels in PDMS-based microfluidic devices. The coatings are long-term stable and allow for the generation of monodisperse oil-in-water microdroplets even several months after the channel surface treatment. Due to the robustness of the polyelectrolyte multilayers ultra-high flow rates can be applied, making high-throughput droplet formation in the jetting mode possible. Furthermore, we successfully used our method to selectively modify the surface properties in certain areas of assembled microchannels. The resulting partially hydrophilic, partially hydrophobic microfluidic devices allow for the production of monodisperse water-in-oil-in-water double emulsions.

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“…PDMS devices are fabricated by mixing a viscous prepolymer with a curing agent to induce polymerization [121] and the mixture is cast and cured on microfabricated molds. Mixing PDMS with functional molecules enables the material surface to be crosslinked to other chemical species via the added reactive surface functional groups [123–126]. This method has high potential for binding proteins to PDMS surfaces, but it has not yet been developed to fabricate devices for robust cell culture.…”

Section: Enabling Cell Adhesion By Engineering Surface-ecm Proteinmentioning

confidence: 99%

For whom the cells pull: Hydrogel and micropost devices for measuring traction forces

Ribeiro

Denisin

Wilson

et al. 2016

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While performing several functions, adherent cells deform their surrounding substrate via stable adhesions that connect the intracellular cytoskeleton to the extracellular matrix. The traction forces that deform the substrate are studied in mechanotrasduction because they are affected by the mechanics of the extracellular milieu. We review the development and application of two methods widely used to measure traction forces generated by cells on 2D substrates: i) traction force microscopy with polyacrylamide hydrogels and ii) calculation of traction forces with arrays of deformable microposts. Measuring forces with these methods relies on measuring substrate displacements and converting them into force. We describe approaches to determine force from displacements and elaborate on the necessary experimental conditions for this type of analysis. We emphasize device fabrication, mechanical calibration of substrates and covalent attachment of extracellular matrix proteins to substrates as key features in the design of experiments to measure cell traction forces with polyacrylamide hydrogels or microposts. We also report the challenges and achievements in integrating these methods with platforms for the mechanical stimulation of adherent cells. The approaches described here will enable new studies to understand cell mechanical outputs as a function of mechanical inputs and the understanding of mechanotransduction mechanisms.

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Surface modification of PDMS via self-organization of vinyl-terminated small molecules

Cited by 32 publications

References 43 publications

Versailles Project on Advanced Materials and Standards Interlaboratory Study on Measuring the Thickness and Chemistry of Nanoparticle Coatings Using XPS and LEIS

Versailles Project on Advanced Materials and Standards Interlaboratory Study on Measuring the Thickness and Chemistry of Nanoparticle Coatings Using XPS and LEIS

Hydrophilic PDMS microchannels for high-throughput formation of oil-in-water microdroplets and water-in-oil-in-water double emulsions

For whom the cells pull: Hydrogel and micropost devices for measuring traction forces

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