Surface modification of poly(butylene terephthalate) nonwoven by photochemistry and biofunctionalization with peptides for blood filtration

Abstract

The surface of meltblown poly(butylene terephthalate) (PBT) nonwoven was modified by photochemistry using the photolinker O‐succinimidyl 4‐azido‐2,3,5,6‐tetrafluorobenzoate for the introduction of activated ester functions and then coupling of molecular probes or biomolecules. Approximately 4000 pmol of (L)‐4,5‐[3H]‐lysine was fixed per PBT sample (1.13 cm2) and measured by liquid scintillation counting. The method consisted in a two‐step process: (a) coating of the clip (0.05 mg/sample) on the fibrous surface of the PBT followed by UV irradiation (30 min, 254 nm) and (b) coupling of amine‐terminated molecules (10−3 M in phosphate buffer–CH3CN [1/1, v/v], 20 h). Moreover, about 2000 pmol of 3H‐lysine can be immobilized on the PBT surface after UV irradiation (without clip) by aminolysis reactions with the created oxygenated functions. The derivatizations (via the clip and UV irradiation only) were stable after long‐term heating at 100 °C in water or under steam‐sterilization conditions. They induce neither modifications of the nonwoven morphology nor cytotoxicity. This method was applied for the grafting of peptides Gly‐Arg‐Gly‐Asp‐Ser and Gly‐Gly‐Gly‐Gly‐Gly to perform blood filtration experiments and to retain the leukocytes. © 2011 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem, 2011

Perfluorophenyl azide functionalization of electrospun poly(para‐dioxanone)

“…This method allows the coupling of a variety of primary amine‐containing bioactive molecules such as peptides and proteins: Examples are the coupling of RGD peptides allowing for nonselective adhesion of cells via their integrins, or the immobilization of enzymes and cell contact‐mediating proteins on PFPA‐functionalized surfaces. Moreover, the NHS ester moieties introduced by this method can be used to incorporate also other functional groups, eg, azides or alkynes, that have successfully been used for carbohydrate immobilization via “click” chemistry …”

“…Perfluorophenyl azides form highly reactive nitrene intermediates under UV irradiation, and the latter readily undergo C─H insertion reactions with hydrocarbon‐based materials . Only a few studies report on using this methodology for surface functionalization of porous polymeric scaffolds, eg, melt‐blown poly(butylene terephthalate) (PBT) nonwoven fiber meshes or poly(4‐methyl‐1‐pentene) membranes . It was envisioned that the versatile PFPA chemistry can be used by inserting the perfluorophenyl nitrene intermediate into the aliphatic groups of PPDO.…”

Investigation of the Characteristics of Elasticised Woven Fabric by Using PBT Filament Yarns

“…According to the literature survey, researches with respect to PBT are mainly focused on nonwoven production, particularly for filtration purposes [5][6][7][8][9][10][11][12][13][14][15]. Besides, an artificial neural network model was developed to predict the seam strength and elongation at break values and cotton, core-spun, and PBT sewing yarns have been used for experimental.…”

Surface Functionalization of Electrospun Poly(butylene terephthalate) Fibers by Surface-Initiated Radical Polymerization

“…Polyester microfibers have potential applications for filtration media and tissue scaffolds because of their high surface area to volume ratio. For medical and environmental applications, it is also desirable to modify the wetting, adhesion, and antifouling properties, which can be controlled through surface modification . Oxidation by corona discharge treatment or plasma treatment is a common approach to improve the hydrophilicity .…”

“…For example, surface‐active compounds and diblock copolymer surfactants containing fluoroalkyl groups spontaneously segregate at the fiber surface during the spinning process, and can be used to prepare superhydrophobic nonwoven fiber mats. Surface modification can also be achieved by introducing reactive groups at the fiber surfaces and subsequently reacting with functional molecules . However, the surface properties introduced by the above‐mentioned procedures tend to rapidly degrade due to surface migration and elimination of the additive compounds.…”