Surface Modification of Polymeric Scaffolds for Tissue Engineering Applications

Sengupta, Poulomi; Prasad, Bhagavatula L. V.

doi:10.1007/s40883-018-0050-6

Cited by 20 publications

(26 citation statements)

References 109 publications

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“…Our previous results suggest that the primary amine groups of the amino acids electrostatically interact with the citrate groups on the gold nanoparticles. 17 The interaction is stronger if the amino acids have more than one free amine group, which can have a constructive interaction with the citrate capping present on the gold nanoparticle surface. Although our previous results suggested lysine as a good surface-coating agent, and provided enhanced cell adhesion and proliferation, 17 as a part of this work several other amino acids were also tried (data not shown).…”

Section: Discussionmentioning

confidence: 99%

“… 17 The interaction is stronger if the amino acids have more than one free amine group, which can have a constructive interaction with the citrate capping present on the gold nanoparticle surface. Although our previous results suggested lysine as a good surface-coating agent, and provided enhanced cell adhesion and proliferation, 17 as a part of this work several other amino acids were also tried (data not shown). It was concluded that positively charged amino acid arginine adsorbs very strongly to gold nanoparticles, resulting in a super hydrophilic yet rough surface, which is expected to increase the cellular adhesion properties, as confirmed from the contact angle measurements described below.…”

Section: Discussionmentioning

confidence: 99%

“…In contrast, with only plasma treated films, the hydrophobic character got regenerated within a week. 17 …”

Section: Introductionmentioning

confidence: 99%

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Surface Modification of Polymers for Tissue Engineering Applications: Arginine Acts as a Sticky Protein Equivalent for Viable Cell Accommodation

Sengupta

Prasad

2018

ACS Omega

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Hydrophobic polymers, for their favorable mechanical properties, are a popular choice as permanent bioimplants. These materials remain absolutely bioinert for years, but throw up challenges when it comes to fast integration with healthy tissue. Addressing this, herein, we present a surface-modification technique of converting the hydrophobic surface of a polymeric film into a hydrophilic one using a layer-by-layer assembly process involving gold nanoparticles and small molecules like amino acids. These films showed much improved animal cell (murine fibroblast) adherence properties compared to commercially available tissue culture plates. Moreover, arginine-modified films exhibited a nearly equivalent cell viability compared to the films modified with the natural extracellular matrix component fibronectin. The surface hydrophilicity and roughness of our novel film were characterized by contact angle measurement and atomic force microscopy. Cell counting, fluorescence microscopy, cell viability, and collagen estimation assay were employed to demonstrate that our film favored a much improved cell adherence, and accommodation in comparison to the commercially available tissue culture plates.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Surface Modification of Polymers for Tissue Engineering Applications: Arginine Acts as a Sticky Protein Equivalent for Viable Cell Accommodation

Sengupta

Prasad

2018

ACS Omega

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“…Some of us earlier reported a procedure where with the help of plasma treatment and metallic nanoparticles, this shortcoming could be circumvented. 15 , 19 We followed a similar strategy 20 , 21 to design the dual-functional scaffold as part of this study. The specific details of the scaffold preparation are detailed below ( Scheme 1 ).…”

Section: Resultsmentioning

confidence: 99%

Development of a Smart Scaffold for Sequential Cancer Chemotherapy and Tissue Engineering

Sengupta

Agrawal²,

Prasad

2020

ACS Omega

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The fabrication of a dual-functional drug-containing porous polymeric scaffold by layer-by-layer surface modification involving citrate-stabilized gold nanoparticles and cisplatin molecules is being reported. These scaffolds were characterized by electron microscopy and X-ray photoelectron spectroscopy. The capability of the scaffolds to release hydrated cisplatin in a slow and sustained manner over two days is established. Most importantly, the scaffolds turn nontoxic and cell-friendly after drug release, thus allowing the noncancerous fibroblast cells to adhere and proliferate (from 5000 cells to 16,000 cells in 6 days), becoming a potential solution toward an effective drug-carrying scaffold for volume-filling applications. The scaffold-mediated cancer cell killing and fibroblast cell proliferation were confirmed by fluorescence microscopy imaging, flow cytometry, and cell proliferation assays. We surmise that such a dual-purpose (drug-delivery and volume-filler) scaffold could help avoid the multiple surgical interventions needed for tumor surgery and cosmetic corrections. To the best of our knowledge, this is the first example of scaffolds with such a dual functionality which gets manifested in a sequential manner.

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“…g. The implant material should be biodegradable and bioresorbable (if the bioimplant is meant to temporarily replace an organ) [30]. h. It should be free from contamination and should be easily moldable into various shapes and sizes [30].…”

Section: Craniomaxillofacial Bone Defects and Reconstruction Strategiesmentioning

confidence: 99%

Advances in Tissue Engineering Approaches for Craniomaxillofacial Bone Reconstruction

Tomar¹,

Dave²,

Chandekar³

et al. 2021

Biomechanics and Functional Tissue Engineering

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Trauma, congenital abnormalities and pathologies such as cancer can cause significant defects in craniofacial bone. Regeneration of the bone in the craniofacial area presents a unique set of challenges due to its complexity and association with various other tissues. Bone grafts and bone cement are the traditional treatment options but pose their own issues with regards to integration and morbidity. This has driven the search for materials which mimic the natural bone and can act as scaffolds to guide bone growth. Novel technology and computer aided manufacturing have allowed us to control material parameters such as mechanical strength and pore geometry. In this chapter, we elaborate the current status of materials and techniques used in fabrication of scaffolds for craniomaxillofacial bone tissue engineering and discuss the future prospects for advancements.

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Surface Modification of Polymeric Scaffolds for Tissue Engineering Applications

Cited by 20 publications

References 109 publications

Surface Modification of Polymers for Tissue Engineering Applications: Arginine Acts as a Sticky Protein Equivalent for Viable Cell Accommodation

Surface Modification of Polymers for Tissue Engineering Applications: Arginine Acts as a Sticky Protein Equivalent for Viable Cell Accommodation

Development of a Smart Scaffold for Sequential Cancer Chemotherapy and Tissue Engineering

Advances in Tissue Engineering Approaches for Craniomaxillofacial Bone Reconstruction

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