Surface modification on polycaprolactone electrospun mesh and human decalcified bone scaffold with synovium-derived mesenchymal stem cells-affinity peptide for tissue engineering

Abstract

Synovium-derived mesenchymal stem cells (SMSC) have been studied for over a decade since first being successfully isolated in 2001. These cells demonstrate the most promising therapeutic efficacy for musculoskeletal regeneration of the MSC family, particularly for cartilage regeneration. However, the mobilization and transfer of MSCs to defective or damaged tissues and organs in vivo with high accuracy and efficiency has been a major problem in tissue engineering (TE). In the present study, we identified a seven amino acid peptide sequence [SMSCs-affinity peptide (LTHPRWP; L7)] through phage display technology that has a high specific affinity to SMSCs. Our analysis suggested that L7 efficiently and specifically interacted with SMSCs without any species specificity. Thereafter, L7 was covalently conjugated onto both polycaprolactone (PCL) electrospun meshes and human decalcified bone scaffolds (hDBSc) to investigate its TE applications. After 24 h coculture with human SMSCs (hSMSCs), L7-conjugated PCL electrospun meshes had significantly more adherent hSMSCs than the control group, and the cells expanded well. Similar results were obtained using hDBSs. These results suggest that the novel L7 peptide sequence has a high specific affinity to SMSCs. Covalently conjugating this peptide to either artificial polymer material (PCL mesh) or natural material (hDBS) significantly enhances the adhesion of SMSCs. This method is applicable to a wide range of potential SMSC-based TE applications, particularly to cartilage regeneration, via surface modification on various type of materials. V C 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 00A:000-000, 2014.

Repair and tissue engineering techniques for articular cartilage

“…The study found the PBSC group had improved quality of cartilage as shown in the ICRS visual assessment scale histology score and an MRI-based morphological score over those of the control group 18 months after injection. 77 In vitro , synovial-derived stem cells have a better chondrogenic potential than stem cells from other sources, 78,79 as shown by the development of hyaline-like neotissue when they are seeded onto scaffolds, 80 although clinical studies using these cells have not yet been conducted. Multilineage dermal stem cells have also been isolated and have a high capacity for in vitro chondroinduction in a cartilage tissue engineering study.…”

Application of permselective poly(ε-caprolactone) film for equilibrium-modified atmosphere packaging of strawberry in cold storage

“…It is an important polymer due to its biodegradability and mechanical properties (Tokiwa, Calabia, Ugwu, & Aiba, 2009). PCL has been used in different fields such as scaffolds in microelectronics (Gomes et al, 2015;Li, Ruan, Zhang, & Rong, 2015), tissue engineering and drug delivery systems (Dash & Konkimalla, 2012;Diab, Jaafar-Maalej, Fessi, & Maincent, 2012;Remya et al, 2013;Shao et al, 2015), as adhesives and in packaging but not in food packaging due to the low melting point of the polymer. For fresh food, storage and transportation always combined with cold storage.…”

A co-culture system of rat synovial stem cells and meniscus cells promotes cell proliferation and differentiation as compared to mono-culture

“…To verify the purity of SMSCs, we used flow cytometry to test cell surface antigen markers. The results revealed that the SMSCs consisted of a single phenotypic population positive for CD44 (98.64%), CD29 (98.23%), and CD90 (98.94%) 18 – 20 . By contrast, the cells were negative for other markers of the hematopoietic lineage, including lipopolysaccharide receptor CD34 (7.88%) and the leukocyte common antigen CD45 (4.03%), indicating their non-hematopoietic origin (Fig.…”