Surface modifications by gas plasma control osteogenic differentiation of MC3T3-E1 cells

Barradas, Ana; Lachmann, Kristina; Hlawacek, Gregor; Frielink, Cathelijne; Truckenmoller, Roman; Boerman, Otto C.; Gastel, Raoul van; Garritsen, Henk; Thomas, Michael; Moroni, Lorenzo; Blitterswijk, Clemens A. van; Boer, Jan de

doi:10.1016/j.actbio.2012.04.021

Cited by 39 publications

(31 citation statements)

References 48 publications

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“…10 Charged molecules and radicals form after the application of high voltage. These reactive species interact with the surfaces of materials and promote the incorporation of functional groups.…”

Section: Discussionmentioning

confidence: 99%

“…In contrast, the recently developed atmospheric-pressure plasma (APP) technology is advantageous because it does not require a vacuum, thus reducing the processing time. 10 When the Ti surface experienced various types of APP treatments, the APP eliminated surface carbon contaminations (i.e., activation), increased surface wettability, facilitated adsorption of FN and, consequently, facilitated cell adhesion and proliferation, including those of osteoblastic cells. 5,8,[11][12][13][14] However, to our knowledge, detailed mechanistic investigations from a biological perspective have not been performed.…”

Section: Introductionmentioning

confidence: 99%

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An atmospheric‐pressure plasma‐treated titanium surface potentially supports initial cell adhesion, growth, and differentiation of cultured human prenatal‐derived osteoblastic cells

et al. 2014

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An atmospheric-pressure plasma (APP) treatment was recently reported to render titanium (Ti) surfaces more suitable for osteoblastic cell proliferation and osteogenesis. However, the mechanism of action remains to be clearly demonstrated. In this study, we focused on cell adhesion and examined the effects of the APP treatment on the initial responses of human prenatal-derived osteoblastic cells incubated on chemically polished commercially pure Ti (CP-cpTi) plates. In the medium containing 1% fetal bovine serum, the initial cell adhesion and the actin polymerization were evaluated by scanning electron microscopy and fluorescence microscopy. The expression of cell adhesion-related molecules and osteoblast markers at the messenger RNA level was assessed by real-time quantitative polymerase chain reaction. Although the cells on the APP-treated CP-cpTi surface developed fewer cytoskeletal actin fibers, they attached with higher affinity and consequently proliferated more actively (1.46-fold over control at 72 h). However, most of the cell adhesion molecule genes were significantly downregulated (from 40 to 85% of control) in the cells incubated on the APP-treated CP-cpTi surface at 24 h. Similarly, the osteoblast marker genes were significantly downregulated (from 49 to 63% of control) at 72 h. However, the osteoblast marker genes were drastically upregulated (from 197 to 296% of control) in these cells by dexamethasone and β-glycerophosphate treatment. These findings suggest that the APP treatment improves the ability of the CP-cpTi surface to support osteoblastic proliferation by enhancing the initial cell adhesion and supports osteoblastic differentiation when immature osteoblasts begin the differentiation process.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

An atmospheric‐pressure plasma‐treated titanium surface potentially supports initial cell adhesion, growth, and differentiation of cultured human prenatal‐derived osteoblastic cells

et al. 2014

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“…For example, human umbilical vein endothelial cell (HUVEC) adhesion was improved by plasma treatment of PLA through the control of carbon and oxygen concentration [35], and human embryonic palatal mesenchyme (HEPM) cell proliferation was increased by plasma-treated poly(ether ether ketone) (PEEK) through assembling amino groups on the surface [36]. Amino-rich PLA surfaces created by plasma treatment were also reported to promote osteogenic differentiation of MC3T3-E1 cells [32]. More recently, Liu et al investigated the effects of plasma treatment on the surface of PLLA nanofibrous membranes, and the subsequent dose-dependent cellular response and osteogenesis of MSCs were clarified preliminary [37].…”

Section: Plasma Treatmentmentioning

confidence: 99%

“…Low-temperature plasma treatment has been shown to be a convenient and effective way to modify surfaces to improve the hydrophilicity of biomaterials, thus increasing their biocompatibility and facilitate cell attachment [31,32]. Wan et al reported that ammonia plasma treatment significantly increased the hydrophilicity of PLA scaffolds and resulted in enhanced cell adhesion and proliferation of mouse 3T3 fibroblasts [33].…”

Section: Plasma Treatmentmentioning

confidence: 99%

Biological Events Occuring on the Biosis–Abiosis Interface: Cellular Responses Induced by Implantable Electrospun Nanofibrous Scaffolds

Deng

Wei

Zhang

et al. 2015

Interface Oral Health Science 2014

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Electrospun nanofibers have tremendous potential as novel scaffolds for tissue engineering of various soft and hard tissues because of thier high surface area, surface functional groups, interconnected pores, and nano-scaled size. In this chapter, we reviewed the types of the nanofibrous scaffolds that have been used as implantable biomedical devices and used electrospun nanofibrous guided tissue regeneration membrane as an example to illustrate how the physiochemical properties of nanofibrous scaffolds influenced the biological events occuring on the scaffolds-host interface. It could be concluded that physical and chemical stimuli caused by nanofibrous scaffold would support in vivo-like three-dimensional cell adhesion and activate cell-signaling pathways. In terms of physical stimulus, the process of mechanotransduction may play an important role in influencing cellular behaviors. A result, biological events such as cell-interface recognition, cell proliferation, and cell differentiation are altered. Nevertheless, the cellular and molecular mechanisms by which cells sense and respond to nanofiberous scaffolds remain poorly understood. More evidences are needed to reveal the underlying mechanisms whereby environmental cues alternated the cellular responses to the

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“…Plasma treatment has been widely used since it rarely involves chemical reagents harmful to cells and biological tissues. Moreover, it induces both physical and chemical changes in the beneficial bulk properties of the materials, e.g., surface chemical composition, roughness and wettability, which directly govern the cellular behaviors and functions [5], [6].…”

Section: Introductionmentioning

confidence: 99%

Chondrocyte Infiltration and ECM Production on Surface- Treated PCL Scaffolds: Alkaline Hydrolysis versus Plasma Treatment

Kaewkong¹,

Uppanan²,

Thavornyutikarn³

et al. 2013

IJBBB

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Abstract-The objective of this study was to comparatively examine the responses of porcine chondrocytes to two different polycaprolactone (PCL) scaffolds whose surfaces were treated by alkaline hydrolysis and low pressure oxygen (O 2 ) plasma treatment, namely HPCL and plasma-treated PCL scaffolds, respectively. The surface morphology and the hydrophilicity of both scaffolds were evaluated by scanning electron microscopy (SEM) and a water contact angle measurement, respectively. The chondrocytes cultured on each scaffold were assessed for their proliferation, cartilage-specific gene expression, cell infiltration, and extracellular matrix (ECM) synthesis after a 21-day culture period. The scanning electron micrographs revealed the increased roughness of both HPCL and plasmatreated PCL scaffolds compared with the untreated PCL scaffold. The measured water contact angle of the plasmatreated PCL scaffold appeared much smaller than that on the HPCL scaffold. The chondrocytes cultured on the HPCL and plasma-treated PCL scaffolds exhibited an insignificant difference in cell proliferation. The expression of type II collagen and aggrecan mRNA found on both surface-treated scaffolds was not much different, either. Nevertheless, the histological results demonstrated that the chondrocytes on the plasma-treated PCL scaffold could more thoroughly infiltrate into the inner parts of the scaffold than those on the HPCL scaffold. Furthermore, a greater ECM production was observed on the plasma-treated PCL scaffold.

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Surface modifications by gas plasma control osteogenic differentiation of MC3T3-E1 cells

Cited by 39 publications

References 48 publications

An atmospheric‐pressure plasma‐treated titanium surface potentially supports initial cell adhesion, growth, and differentiation of cultured human prenatal‐derived osteoblastic cells

An atmospheric‐pressure plasma‐treated titanium surface potentially supports initial cell adhesion, growth, and differentiation of cultured human prenatal‐derived osteoblastic cells

Biological Events Occuring on the Biosis–Abiosis Interface: Cellular Responses Induced by Implantable Electrospun Nanofibrous Scaffolds

Chondrocyte Infiltration and ECM Production on Surface- Treated PCL Scaffolds: Alkaline Hydrolysis versus Plasma Treatment

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