Surface modifications for enhanced enzyme immobilization and improved electron transfer of PQQ-dependent glucose dehydrogenase anodes

Integration of Platinum Group Metal‐Free Catalysts and Bilirubin Oxidase into a Hybrid Material for Oxygen Reduction: Interplay of Chemistry and Morphology

“…[50,51] Studiesp reviously conducted by our group demonstratedt hat the significant positive effect observed is owed to an enzyme-substrate recognitione vent and subsequent propere nzymeo rientation, [62] not to surface chemistry alone. [50,51] Studiesp reviously conducted by our group demonstratedt hat the significant positive effect observed is owed to an enzyme-substrate recognitione vent and subsequent propere nzymeo rientation, [62] not to surface chemistry alone.…”

“…It is interesting to mention that modification of carbon electrodes with ap yrrole-containing organicc ompound (bilirubin) was found to have ar emarkable positive effect on BOx cathodes. [50,51] Studiesp reviously conducted by our group demonstratedt hat the significant positive effect observed is owed to an enzyme-substrate recognitione vent and subsequent propere nzymeo rientation, [62] not to surface chemistry alone. The modificationp rocedure wasb eneficial for positioning BOx closer relative to the support by providing optimal orientation of the enzyme and facilitating the interfacial electron transfer by decreasing the distance betweent he electrode surface and the T1 Cu.…”

“…[50,51] To maximize the performance of ah ybrid catalystb ased on PGM-free materiala nd BOx, the effects of their physiochemicalc haracteristics of the PGM-free catalystwere examined. [50,51] To maximize the performance of ah ybrid catalystb ased on PGM-free materiala nd BOx, the effects of their physiochemicalc haracteristics of the PGM-free catalystwere examined.…”

“…It was previously demonstrated that there can be as ignificant increasei np erformance when PGM-free catalysts are integrated with enzymes andt hat the performanced epends on the integration conditions. [50,51] To maximize the performance of ah ybrid catalystb ased on PGM-free materiala nd BOx, the effects of their physiochemicalc haracteristics of the PGM-free catalystwere examined.…”

Pyrroloquinoline quinone-dependent glucose dehydrogenase bioelectrodes based on one-step electrochemical entrapment over single-wall carbon nanotubes

“…The design of enzymatic bioelectrodes has received great attention as a fundamental part in technologies for green-energy production based on electrochemical biofuel cells (EBFC), food chemistry and biosensing [1][2][3]. In this sense, the current research has mainly focused in the development of scalable, controlled and reproducible procedures for the synthesis of biologically modified surfaces, which can provide active bioelectrodes with efficient electron-transfer (ET) between the enzyme and the electrode, as well as high sensitivity and stability [4][5][6]. Among the possible alternatives, the development of architectures providing direct electron transfer (DET) between the biocatalyst (i.e., enzyme) and the electrode surface is considered as a promising way to ensure high turnover rates of the redox enzyme and high kinetics rates of the ET process [7,8]; while at the same time avoiding the use of external redox mediators (e.g., NAD + ).…”