Surface Modifications of Nanoparticles for Stability in Biological Fluids

Guerrini, Luca; Alvarez-Puebla, Ramón A.; Pazos-Perez, Nicolas

doi:10.3390/ma11071154

Cited by 410 publications

(254 citation statements)

References 165 publications

(236 reference statements)

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“…Biological fluids are complex mixtures of biomolecules in aqueous solvent containing high concentrations of small ions, and formation of a protein corona can markedly affect the colloidal stability of NPs . A dense adsorption layer of globular proteins is highly hydrophilic due to its polyzwitterionic nature and can enhance colloidal stability of NPs.…”

Section: Introductionmentioning

confidence: 99%

Formation of a Monolayer Protein Corona around Polystyrene Nanoparticles and Implications for Nanoparticle Agglomeration

Wang

Nienhaus

et al. 2019

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Nanoparticle (NP) interactions with cells and organisms are mediated by a biomolecular adsorption layer, the so‐called “protein corona.” An in‐depth understanding of the corona is a prerequisite to successful and safe application of NPs in biology and medicine. In this work, earlier in situ investigations on small NPs are extended to large polystyrene (PS) NPs of up to 100 nm diameter, using human transferrin (Tf) and human serum albumin (HSA) as model proteins. Direct NP sizing experiments reveal a reversibly bound monolayer protein shell (under saturating conditions) on hydrophilic, carboxyl‐functionalized (PS‐COOH) NPs, as was earlier observed for much smaller NPs. In contrast, protein binding on hydrophobic, sulfated (PS‐OSO3H) NPs in solvent of low ionic strength is completely irreversible; nevertheless, the thickness of the observed protein corona again corresponds to a protein monolayer. Under conditions of reduced charge repulsion (higher ionic strength), the NPs are colloidally unstable and form large clusters below a certain protein–NP stoichiometric ratio, indicating that the adsorbed proteins induce NP agglomeration. This comprehensive characterization of the persistent protein corona on PS‐OSO3H NPs by nanoparticle sizing and quantitative fluorescence microscopy/nanoscopy reveals mechanistic aspects of molecular interactions occurring during exposure of NPs to biofluids.

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Section: Introductionmentioning

confidence: 99%

Formation of a Monolayer Protein Corona around Polystyrene Nanoparticles and Implications for Nanoparticle Agglomeration

Wang

Nienhaus

et al. 2019

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“…To maintain MNP stability in aqueous environment, they are typically coated with molecules anchored to their surface via a chelating function. [20][21][22] Among available iron chelating agents, citric acid is one of the most used coating agent in biomedical applications. [23][24][25] This last decade, phosphonate and catechol also appeared amongst effective iron chelating agents.…”

Section: Introductionmentioning

confidence: 99%

Impact of magnetic nanoparticle surface coating on their long-term intracellular biodegradation in stem cells

et al. 2019

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Magnetic nanoparticles (MNP) internalized within stem cells have paved the way for remote magnetic cell manipulation and imaging in regenerative medicine. A full understanding of their interactions with stem cells and of their fate in the intracellular environment is then required, in particular with respect to their surface coatings. Here, we investigated biological interactions of MNP composed of an identical magnetic core but coated with different molecules: phosphonoacetic acid, polyethylene glycol phosphonic carboxylic acid, caffeic acid, citric acid, and polyacrylic acid. These coatings vary in the nature of the chelating function, the number of binding sites, and the presence or absence of a polymer. The nanoparticles magnetism was systematically used as an indicator of their internalization within human stem cells and of their structural long-term biodegradation in a 3D stem cell spheroid model. Overall, we evidence that the coating impacts the aggregation status of the nanoparticles and subsequently their uptake within stem cells, but has little effect on their intracellular degradation. Only a high number of chelating functions (polyacrylic acid) had a significant protective effect. Interestingly, when the nanoparticles aggregated prior to cellular internalization, a lower degradation was also demonstrated. Finally, for all coatings, a robust dose-dependent intracellular degradation rate was demonstrated, with higher doses of internalized nanoparticles leading to lower degradation extent.

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“…Of note, this maximum level is particularly high compared to MRLs of other mycotoxins in cereals (5 µg/kg for ochratoxin A, 4 µg/kg for total aflatoxins…) (EC, 2006). For that reason, we chose gold nanoparticles, which have high stability, as probes (Guerrini et al, 2018;Sajid et al, 2015).…”

Section: General Commentsmentioning

confidence: 99%

Development of an IgY-based lateral flow immunoassay for detection of fumonisin B in maize

et al. 2019

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Fumonisins are among the most prevalent mycotoxins in maize, causing substantial economic losses and potential health risks in humans and animals. In the present study, in-house polyclonal IgY antibody against fumonisin B1 (FB ) and B2 (FB ) was applied for the development of a competitive lateral flow immunoassay detecting these mycotoxins in maize grains with the limit of detection of 4000 µg/kg, which corresponds to the maximum residue limit adopted by the European Commission. To this end, factors affecting the test performance including nitrocellulose membrane type, dilution factor of maize homogenates in running buffer, amount of detection conjugate, and incubation time between detection conjugate and samples were optimized. Under the optimal condition (UniSart CN140 nitrocellulose membrane, FB -BSA immobilized at 1 µg/cm, 1:10 dilution factor, 436 ng of gold nanoparticle conjugate, 30 minutes of incubation), the developed test could detect both FB and FB in maize with limit of detection of 4000 µg/kg, and showed no cross-reactivity to deoxynivalenol, ochratoxin A, aflatoxin B1 and zearalenone. When applied to detect FB and FB in naturally contaminated maize samples, results obtained from the developed assay were in good agreement with those from the high-performance liquid chromatography method. This lateral flow immunoassay is particularly suitable for screening of fumonisins in maize because of its simplicity and cost-effectiveness.

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Surface Modifications of Nanoparticles for Stability in Biological Fluids

Cited by 410 publications

References 165 publications

Formation of a Monolayer Protein Corona around Polystyrene Nanoparticles and Implications for Nanoparticle Agglomeration

Formation of a Monolayer Protein Corona around Polystyrene Nanoparticles and Implications for Nanoparticle Agglomeration

Impact of magnetic nanoparticle surface coating on their long-term intracellular biodegradation in stem cells

Development of an IgY-based lateral flow immunoassay for detection of fumonisin B in maize

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