Surface-modified loaded human red blood cells for targeting and delivery of drugs

Sternberg, Nadine; Georgieva, Radostina; Duft, Karolin; Bäumler, Hans

doi:10.3109/02652048.2011.629741

Cited by 33 publications

(20 citation statements)

References 42 publications

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“…This hypotonic hemolysis method is the one most frequently used among the several drug loading techniques investigated, 78 115 since it allows to preserve greatly the cellular biochemical and physiological characteristics of the erythrocytes. 77 116 The encapsulation of citrate-coated SPIO nanoparticles into RBCs by a hypotonic haemolysis method was proposed by Brähler et al 117 and Stenberg et al 118 For loading procedure, RBCs and magnetite nanoparticles were incubated under stirring in hypoosmotic lysing buffer and subsequently resealed by incubation in phosphate buffered saline. The resulting SPIO-loaded RBCs respond to the external magnetic field moving toward an attached magnet and this magnetic response confirms interaction and attachment of magnetic nanoparticles to the cells.…”

Section: Rbcs For the Encapsulation And Delivery Of Magnetic Nanopartmentioning

confidence: 99%

Red Blood Cells as Carriers of Iron Oxide-Based Contrast Agents for Diagnostic Applications

Antonelli¹,

Magnani²

2014

j biomed nanotechnol

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New biomedical strategies are being developed to improve disease detection, therapeutic monitoring, and treatment efficacy in several contexts for example in anti-angiogenic chemotherapy, and prevention of cardiovascular disorders. The use of contrast agents to improve the sensitivity and resolution of diagnostic imaging modalities, detect lesions or organs, and evaluate organ function was immediately recognized. In the last years, superparamagnetic iron oxide (SPIO) nanoparticles have been clinically used as MRI contrast agents for the diagnosis of liver diseases, due to their ability to shorten T 2 * relaxation times. After the intravenous administration, SPIO nanoparticles are rapidly eliminated from the bloodstream since they are selectively taken up by the Kupffer cells in the liver, spleen, and bone marrow thus improving the clinical tumor detection in these tissues. However, for the investigation of vascular system including functional cardiac diagnosis, angiography and cardiac wall motion assessment, contrast agents with long blood circulation time would be necessary. In fact, imaging applications for long-term monitoring require the repeated administration of SPIO bolus injections, which complicates quantitative comparisons due to the temporal variations in concentration. In this context, researchers have developed several SPIO delivery systems based on red blood cells (RBCs) used as carriers to increase the blood circulation time of contrast agents. Encapsulation of SPIOs into RBCs appears the most attractive strategy to obtain magnetic RBC-constructs suitable for MRI and MPI analyses of blood vascular system.

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Section: Rbcs For the Encapsulation And Delivery Of Magnetic Nanopartmentioning

confidence: 99%

Red Blood Cells as Carriers of Iron Oxide-Based Contrast Agents for Diagnostic Applications

Antonelli¹,

Magnani²

2014

j biomed nanotechnol

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“…Numerous studies show, that erythrocytes can be used as vehicles for bioactive agents, therapeutics can be loaded into the cells, or coupled onto their surfaces [9][10][11]. The advantage of this approach is that they are of natural origin, thereby serving as biocompatible carriers.…”

Section: Introductionmentioning

confidence: 99%

Physicochemical characterization of artificial nanoerythrosomes derived from erythrocyte ghost membranes

Deák

Mihály

Szigyártó

et al. 2015

Colloids and Surfaces B: Biointerfaces

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Colloidal stabile nanoerythrosomes with 200 nm average diameter were formed from hemoglobin-free erythrocyte ghost membrane via sonication and membrane extrusion. The incorporation of extra lipid (1,2-dipalmitoyl-sn-glycero-3-phosphocholine, DPPC), added to the sonicated ghosts, caused significant changes in the thermotropic character of the original membranes. As a result of the increased DPPC ratio the chain melting of the hydrated DPPC system and the characteristic small angle X-ray scattering (SAXS) of the lipid bilayers appeared. Significant morphological changes were followed by transmission electron microscopy combined with freeze fracture method (FF-TEM). After the ultrasonic treatment the large entities of erythrocyte ghosts transformed into nearly spherical nanoerythrosomes with diameters between 100-300 nm and at the same time a great number of 10-30 nm large membrane proteins or protein clusters were dispersed in the aqueous medium. The infrared spectroscopy (FT-IR) pointed out, that the sonication did not cause changes in the secondary structures of the membrane proteins under our preparation conditions. About fivefold of extra lipid -compared to the lipid content of the original membrane -caused homogeneous dispersion of nanoerythrosomes however the shape of the vesicles was not uniform. After the addition of about tenfold of DPPC, monoform and monodisperse nanoerythrosomes became typical. The outer surfaces of these roughly spherical objects were frequently polygonal, consisting of a net of pentagons and hexagons..

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“…The main challenges of these approaches are the loss of RBC’s compatibility and the need for extracorporeal loading. As such, surface loading of nanocarriers to RBCs seems preferable [144]. …”

Section: Particles/carriers Designed For Targeting To or Carriage mentioning

confidence: 99%

Red blood cells: Supercarriers for drugs, biologicals, and nanoparticles and inspiration for advanced delivery systems

Villa

Anselmo

Mitragotri

et al. 2016

Advanced Drug Delivery Reviews

313

227

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Red blood cells (RBCs) constitute a unique drug delivery system as a biologic or hybrid carrier capable of greatly enhancing pharmacokinetics, altering pharmacodynamics (for example, by changing margination within the intravascular space), and modulating immune responses to appended cargoes. Strategies for RBC drug delivery systems include internal and surface loading, and the latter can be performed both ex vivo and in vivo. A relatively new avenue for RBC drug delivery is their application as a carrier for nanoparticles. Efforts are also being made to incorporate features of RBCs in nanocarriers to mimic their most useful aspects, such as long circulation and stealth features. RBCs have also recently been explored as carriers for the delivery of antigens for modulation of immune response. Therefore, RBC-based drug delivery systems represent supercarriers for a diverse array of biomedical interventions, and this is reflected by several industrial and academic efforts that are poised to enter the clinical realm.

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Surface-modified loaded human red blood cells for targeting and delivery of drugs

Cited by 33 publications

References 42 publications

Red Blood Cells as Carriers of Iron Oxide-Based Contrast Agents for Diagnostic Applications

Red Blood Cells as Carriers of Iron Oxide-Based Contrast Agents for Diagnostic Applications

Physicochemical characterization of artificial nanoerythrosomes derived from erythrocyte ghost membranes

Red blood cells: Supercarriers for drugs, biologicals, and nanoparticles and inspiration for advanced delivery systems

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