Surface Modulation of Magnetic Nanocrystals in the Development of Highly Efficient Magnetic Resonance Probes for Intracellular Labeling

Song, Ho Taek; Choi, Ji Ha; Huh, Yong Min; Kim, Sungjun; Jun, Young‐wook; Cheon, Jinwoo

doi:10.1021/ja051833y

Cited by 301 publications

(205 citation statements)

References 23 publications

Supporting

Mentioning

192

Contrasting

Unclassified

Order By: Relevance

“…tissues, blood and single cells). [1][2][3][4][5][6][7][8][9][10][11] In microarray and microspot techniques, spatial resolution of individual reactive sites on a chip is extremely important. At the same time, improved labeling and detection technologies are required to analyze smaller sample volumes and to measure samples on a limited solid-phase area.…”

Section: Introductionmentioning

confidence: 99%

Surface Modification of Silica Nanoparticles to Reduce Aggregation and Nonspecific Binding

2006

View full text Add to dashboard Cite

In this paper, a systematic study of the design and development of surface modification schemes for silica nanoparticles is presented. The nanoparticle surface design involves an optimum balance of the use of inert and active surface functional groups to achieve minimal nanoparticle aggregation and reduce nanoparticle non-specific binding. Silica nanoparticles were prepared in a water-in-oil microemulsion and subsequently surface modified via co-hydrolysis with tetraethylorthosilicate (TEOS) and various organosilane reagents. Nanoparticles with different functional groups, including carboxylate, amine, amine/phosphonate, polyethylene glycol, octadecyl, and carboxylate/octadecyl groups were produced. Aggregation studies, using SEM, dynamic light scattering, and zeta potential analysis, indicate that severe aggregation among amine-modified silica nanoparticles can be reduced by adding inert functional groups, such as methyl phosphonate, to the surface. To determine the effect of various surface modification schemes on nanoparticle non-specific binding, the interaction between functionalized silica nanoparticles and a DNA chip was also studied using confocal imaging/ fluorescence microscopy. Dye-doped silica nanoparticles functionalized with octadecyl and carboxylate groups showed minimal non-specific binding. Using these surface modification schemes, fluorescent dye-doped silica nanoparticles can be more readily conjugated with biomolecules and used as highly fluorescent, sensitive, and reproducible labels in bioanalytical applications.

show abstract

Section: Introductionmentioning

confidence: 99%

Surface Modification of Silica Nanoparticles to Reduce Aggregation and Nonspecific Binding

2006

View full text Add to dashboard Cite

show abstract

“…The usefulness of the multi-component nanoparticle probes is not limited to diagnostic purposes only. These hybrid nanoparticles can be used as probes for biological processes such as cell trafficking and gene delivery under in vitro and in vivo conditions when conjugated with the biologically active species such as stem cells and viruses [17] and [18]. When target specificity and tissue regeneration capabilities are imparted by endocytosis of the magnetic nanoparticles into the stem cells, the composite nanoparticles can be used as dual-function probes possessing both in vivo MRI trafficking and stem cell functions [19].…”

Section: Introductionmentioning

confidence: 99%

The cell labeling efficacy, cytotoxicity and relaxivity of copper-activated MRI/PET imaging contrast agents

et al. 2011

View full text Add to dashboard Cite

/npsi/ctrl?action=rtdoc&an=19739632&lang=en http://nparc.cisti-icist.nrc-cnrc.gc.ca/npsi/ctrl?action=rtdoc&an=19739632&lang=fr READ THESE TERMS AND CONDITIONS CAREFULLY BEFORE USING THIS WEBSITE.http://nparc.cisti-icist.nrc-cnrc.gc.ca/npsi/jsp/nparc_cp.jsp?lang=en Vous avez des questions? Nous pouvons vous aider. Pour communiquer directement avec un auteur, consultez la première page de la revue dans laquelle son article a été publié afin de trouver ses coordonnées. Si vous n'arrivez pas à les repérer, communiquez avec nous à PublicationsArchive-ArchivesPublications@nrc-cnrc.gc.ca. Questions?Contact the NRC Publications Archive team at PublicationsArchive-ArchivesPublications@nrc-cnrc.gc.ca. If you wish to email the authors directly, please see the first page of the publication for their contact information. NRC Publications Archive Archives des publications du CNRCThis publication could be one of several versions: author's original, accepted manuscript or the publisher's version. / La version de cette publication peut être l'une des suivantes : la version prépublication de l'auteur, la version acceptée du manuscrit ou la version de l'éditeur. For the publisher's version, please access the DOI link below./ Pour consulter la version de l'éditeur, utilisez le lien DOI ci-dessous.http://doi.org/10.1016/j.biomaterials.2010.10.013 Biomaterials, 32, 4, pp. 1167Biomaterials, 32, 4, pp. -1176Biomaterials, 32, 4, pp. , 2010 The cell labeling efficacy, cytotoxicity and relaxivity of copperactivated MRI/PET imaging contrast agents AbstractA new class of nanoparticle-based dual-modality positron emission tomography/magnetic resonance imaging (PET/MRI) contrast agents has been developed. The probe consists of a superparamagnetic iron oxide (SPIO) or manganese oxide core coated with 3,4-dihydroxy-d,l-phenylalanine (dl-DOPA). The chelator 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA) was conjugated to DOPA termini. The DOTA modified nanoparticles allow chelation of copper for PET imaging. These surface functionalized nanoparticle-based probes have been characterized by various analytical techniques. The cell-labeling efficacy, cytotoxicity and relaxivity of these nanoparticles have been evaluated and compared with the same properties of one of the most commonly utilized MRI contrast agents, Feridex ® . Evidently, this new nanoparticle has a great potential for use in cell tracking with MRI and PET in the absence of transfecting agent. It is noteworthy that there is a sharp increase in r 2 relaxivity of these nanoparticles on coordination with Cu 2+ ions. Thus these iron oxide nanoparticles can also be explored as the smart magnetic resonance (MR) sensor for the detection of micromolar changes in copper concentration for neurodegenerative diseases such as Alzheimer's disease, Menkes and Wilson's diseases, amyotrophic lateral sclerosis and prion diseases.

show abstract

“…Moreover, the T 2 relaxation rate of 5×10 4 cells is 6.2 s −1 , and still more than twice of the controlled cells (2.3 s −1 ). When used for in vivo monitoring purpose, usually 5×10 5 to 3×10 7 cells were transplanted or injected into small animal models [29,30]. It is possible that SPIO micelle labeled cells would provide noticeable signal contrast to other cells during tracking procedure.…”

Section: Mr and Nirf Imaging Studies Of Labeled Cellsmentioning

confidence: 99%

Near-infrared fluorescent amphiphilic polycation wrapped magnetite nanoparticles as multimodality probes

Wang

Liu

et al. 2012

Chin. Sci. Bull.

View full text Add to dashboard Cite

Construction of multifunctional/multimodality nanoparticles for cancer diagnosis and therapy has become an attractive area of investigation. In this report, we designed a multimodality nanoprobe for cell labeling, and can be detectable by both magnetic resonance and near infrared (NIR) fluorescence imaging. Multiple hydrophobic superparamagnetic iron oxide (SPIO) nanocrystals are self-assembled into nanocomposites in water phase with the help of partially alkylated hyperbranched polycation, polyethylenimine (PEI), which already conjugated with the indocyanine dye Cy5.5 and can be used for cell imaging under NIR fluorescence imaging. The amphiphilic PEI/SPIO nanocomposites have a strong T 2 relaxivity. The iron uptake process in MCF-7/Adr displays a time dependent behavior. Confocal laser scanning microscopy reveals that the nanoprobes are internalized into the cytoplasm of MCF-7/Adr after 24 h labeling. Both MR and NIR fluorescence imaging showed strong image contrast against unlabeled cells. Under a clinical MRI scanner, labeled cells in gelatin phantom present much darker images than controlled ones. The T 2 relaxation rate of the labeled cells is 98.2 s −1 , significantly higher than that of the control ones of 2.3 s −1 . This study provides an important alternative to label MCF-7/Adr at optimized low dosages with high efficiency, and may be useful to label other biologically important cells and track their behaviors in vivo. multifunctional, superparamagnetic iron oxide, multimodality, magnetic resonance imaging, near infrared fluorescence imaging

show abstract

Surface Modulation of Magnetic Nanocrystals in the Development of Highly Efficient Magnetic Resonance Probes for Intracellular Labeling

Cited by 301 publications

References 23 publications

Surface Modification of Silica Nanoparticles to Reduce Aggregation and Nonspecific Binding

Surface Modification of Silica Nanoparticles to Reduce Aggregation and Nonspecific Binding

The cell labeling efficacy, cytotoxicity and relaxivity of copper-activated MRI/PET imaging contrast agents

Near-infrared fluorescent amphiphilic polycation wrapped magnetite nanoparticles as multimodality probes

Contact Info

Product

Resources

About