Surface molecular components of T and B lymphocytes

Mehrishi, J.N.; Zeiller, K.

doi:10.1002/eji.1830040706

Cited by 38 publications

(13 citation statements)

References 30 publications

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“…Moreover, since the mean EPM of both slow and fast moving cells was found to in crease with increasing doses of OHC, it appears that among these two populations, cells are all the more sensitive to OHC as they possess a low electronegative surface charge. However, this does not imply that this ap parent relationship between surface charge, i.e., surface chemical struc ture [6,18] and corticosteroid sensitivity is true in all situations. In fact, Behrens et al [3] have recently reported an inverse correlation in the case of two mouse lymphomas of thymic origin, namely, that the most OHC-sensitive cells possessed a high surface charge as assessed by elec tron microscopy staining with alcian blue, whereas the resistant cells were less charged.…”

Section: Discussionmentioning

confidence: 99%

Dose-Related Effect of Hydrocortisone Treatment on the Electrokinetic Properties and Mitogen Responsiveness of Mouse Thymocytes

Dumont¹,

Robert²

1976

Int Arch Allergy Immunol

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The electrophoretic mobility (EPM) and the ability to synthesize DNA in vitro either spontaneously or in response to concanavalin A (Con A) and phytohemagglutinin (PHA) were investigated on thymocytes from adult female Swiss/B mice which had received intraperitoneally, 2 days previously, various doses (10–750 mg/kg body weight) of hydrocortisone acetate (OHC). A first group of thymocytes, highly sensitive to OHC, rapidly decreased in proportion following administration of increasing doses of OHC (10–125 mg/kg body weight) and included the totality of low-EPM thymocytes (EPM < 1.0 μm sec^––¹ V^––1 cm) together with about 70% of the high-EPM thymocytes encountered in the normal thymus. The second group of thymocytes (4% of the initial cellularity), which resisted to OHC at 125 mg/ kg body weight and was only reduced by half with doses of OHC 6-fold larger, was composed essentially of high-EPM cells. The mean EPM of both groups tended to increase with increasing doses of OHC which suggests that among these two groups thymocytes are all the more OHC-sensitive as they possess a low surface charge. Spontaneous DNA synthesis, measurable on the first 24 h of in vitro cultivation, markedly dropped after treatment with OHC at doses higher than or equal to 62 mg/kg body weight. This loss of early spontaneous proliferation coincided with the disappearance of medium-EPM thymocytes. By contrast, proliferative response of thymocytes to mitogens was enhanced by OHC pretreatment of donors and roughly paralleled the enrichment in high-EPM cells. However, administration of the largest amounts of OHC diminished this mitogen responsiveness, although PHA reactivity was less affected than Con A reactivity.

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Section: Discussionmentioning

confidence: 99%

Dose-Related Effect of Hydrocortisone Treatment on the Electrokinetic Properties and Mitogen Responsiveness of Mouse Thymocytes

Dumont¹,

Robert²

1976

Int Arch Allergy Immunol

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“…On the other hand, EPM is a sensitive parameter for analyzing and monitoring cell membrane events within 1 nm from the surface in saline [7]. Thus, cell electrophoresis can be used to study cell differentiation in a different way from flow cytometry.…”

Section: Epm Of Whole Thymocytesmentioning

confidence: 98%

“…The negative charge on lymphocytes is carried mainly by sialic acid [7,9]. The increase in EPM from DP to SP thymocytes is due to the increased sialic acid content and sialyltransferase activity [ll, 35, 361.…”

Section: Epm Of Whole Thymocytesmentioning

confidence: 99%

Cell electrophoretic characterization of abnormally expanded lymphocytes in autoimmune lpr^cg, lpr, gld and Yaa mice, and of thymocyte subsets

Shimizu

Sekine²,

Matsubara

et al. 1992

Electrophoresis

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Autoimmune mice carrying the lprcg/lprcg(lprcg),lpr/lpr(lpr),gld/gld(gld) and Yaa genes exhibit massive lymphoproliferation and a systemic lupus erythematosus-like syndrome. The surface markers of abnormally expanded lymphocytes used were Thy-1+, CD4-CD8- (double negative, DN) and CD45+ for lprcg, lpr, gld and (lprcg X gld) hybrid (F1-lprcg-gld) mice, and Ig+ for Yaa mice. To characterize the cell surface properties and differentiation pathway of lymphocytes in autoimmune mice, the cell electrophoretic mobility (EPM) was determined for the lymph node (LN), spleen and thymus cells. The EPM of lymphocytes derived from swollen LN was of the T cell type in lprcg, lpr, gld and F1-lprcg-gld mice, but of the B cell type in Yaa mice, indicating that the EPM of abnormally proliferated lymphocytes in autoimmune mice reflects their origin, and that the surface properties detected as a net negative charge were the same in abnormal and normal lymphocytes. The electrophoretic behavior of whole thymocytes was also the same in autoimmune and normal mice. The DN, and CD4+CD8- and CD4-CD8+ (single positive, SP) thymocytes from normal mice exhibited high EPM, while CD4+CD8+ (double positive, DP) thymocytes exhibited low EPM. According to the recent concept of intrathymic T cell differentiation (Schwartz, R. H., Cell. 1989, 57, 1073-1081), it is suggested that EPM of thymocytes may change with maturation in the following manner: DN thymocytes with high EPM----DP thymocytes with low EPM----SP thymocytes and autoimmune DN T cells with high EPM.

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“…Previous data suggesting that in contrast to B cells, mouse lymph node T cells do not bear detectable amounts of SH groups [Mehrishi and Zeiller, 1974b], the question arose whether this property characterizes cells of T lineage.…”

Section: Sulphydryl Groupsmentioning

confidence: 99%

The Lymphocyte Surface

Donner¹,

Mehrishi²

1979

Int Arch Allergy Immunol

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Differences in the electrokinetic properties of thymocytes from A.CA (H-2^f) and A.SW (H-2^S) mice were observed, the anodic electrophoretic mobility (EPM) of H-2^S cells being (9–14%) higher than that of H-2^f cells. The age, sex of the animals and the proportion of hydrocortisone-resistant cells in the thymus did not appear to have any influence on this observation. Studies on thymocytes from F1 hybrid and backcross mice provided the convincing evidence that the differences in the EPMs of H-2^f and H-2^S cells are under genetic control. The EPM of H-2^f/H-2^s heterozygous cells was similar to the EPM of homozygous H-2^f cells. The differences in the EPMs of the H-2^f and H-2^S thymocytes depend upon the striking differences in their surface topochemistry. Compared to the H-2^S thymocyte cell periphery, the H-2^f cells have a substantially higher number (330%) of cationogenic amino groups but only a smaller number (30%) of anionogenic groups (α-carboxyl groups of N-acetylneuraminic acid and phosphate groups susceptible to alkaline phosphatase). The presence of SH groups on the surface of both H-2^f and H-2^S thymocytes was not detectable. It is suggested that the difference in the cell surface topochemistry of thymocytes from the mice investigated is perhaps related to different amounts and/or types of surface membrane molecules under the control of the major histocompatibility complex.

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Surface molecular components of T and B lymphocytes

Cited by 38 publications

References 30 publications

Dose-Related Effect of Hydrocortisone Treatment on the Electrokinetic Properties and Mitogen Responsiveness of Mouse Thymocytes

Dose-Related Effect of Hydrocortisone Treatment on the Electrokinetic Properties and Mitogen Responsiveness of Mouse Thymocytes

Cell electrophoretic characterization of abnormally expanded lymphocytes in autoimmune lpr^cg, lpr, gld and Yaa mice, and of thymocyte subsets

The Lymphocyte Surface

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Surface molecular components of T and B lymphocytes

Cited by 38 publications

References 30 publications

Dose-Related Effect of Hydrocortisone Treatment on the Electrokinetic Properties and Mitogen Responsiveness of Mouse Thymocytes

Dose-Related Effect of Hydrocortisone Treatment on the Electrokinetic Properties and Mitogen Responsiveness of Mouse Thymocytes

Cell electrophoretic characterization of abnormally expanded lymphocytes in autoimmune lprcg, lpr, gld and Yaa mice, and of thymocyte subsets

The Lymphocyte Surface

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Cell electrophoretic characterization of abnormally expanded lymphocytes in autoimmune lpr^cg, lpr, gld and Yaa mice, and of thymocyte subsets