1978
DOI: 10.1007/978-3-642-81246-0_9
|View full text |Cite
|
Sign up to set email alerts
|

Surface Morphology of Lymphoreticular Cells: Review of Data Obtained From Scanning Electron Microscopy

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1

Citation Types

0
3
0

Year Published

1980
1980
2006
2006

Publication Types

Select...
4
2

Relationship

0
6

Authors

Journals

citations
Cited by 7 publications
(3 citation statements)
references
References 71 publications
0
3
0
Order By: Relevance
“…In some cases vacuoles occupied most of the cytoplasmic space and contained amorphous material and membranous structures in a relatively electron-transparent medium. Much data originating from SEM studies have been interestingly controversial and advantageously contributive because of modifications of human leucocyte surface morphology caused by the variations in the techniques used to prepare these cells for electron microscopy observation (Abugaber et al, 1981;Barber & Burkholder, 1975;Corvellec et al, 1983;Lalague et al, 1982;Polliack, 1977Polliack, , 1978Polliack, , 1979Renau-Piqueras et al, 1980;Roath et al, 1978), yet very few reports on the effects of preparatory conditions on the ultrastructure of peripheral blood leucocytes have been available. We have investigated the use of (i) 4% glutaraldehyde (G)+ RpMI-1640 medium, (ii) 4% G+RPMI-1640+0.05 mol sucrose, (iii) 4% G+Hanks, (iv) L4 % G + Hanks + 0.04 ,moll sucrose, (v)] 4%dG + PBS] (phosphate ,buffered saline), (vi) light- Fig* 3.…”
Section: Results a N D Discussionmentioning
confidence: 99%
“…In some cases vacuoles occupied most of the cytoplasmic space and contained amorphous material and membranous structures in a relatively electron-transparent medium. Much data originating from SEM studies have been interestingly controversial and advantageously contributive because of modifications of human leucocyte surface morphology caused by the variations in the techniques used to prepare these cells for electron microscopy observation (Abugaber et al, 1981;Barber & Burkholder, 1975;Corvellec et al, 1983;Lalague et al, 1982;Polliack, 1977Polliack, , 1978Polliack, , 1979Renau-Piqueras et al, 1980;Roath et al, 1978), yet very few reports on the effects of preparatory conditions on the ultrastructure of peripheral blood leucocytes have been available. We have investigated the use of (i) 4% glutaraldehyde (G)+ RpMI-1640 medium, (ii) 4% G+RPMI-1640+0.05 mol sucrose, (iii) 4% G+Hanks, (iv) L4 % G + Hanks + 0.04 ,moll sucrose, (v)] 4%dG + PBS] (phosphate ,buffered saline), (vi) light- Fig* 3.…”
Section: Results a N D Discussionmentioning
confidence: 99%
“…The typical surface morphology of hairy cells visu alized with SEM [5,10,11,15,16,19] has been sug gested to support the diagnosis of HCL. The clear demonstration, however, that at the single cell level, highly developed surface ruffles admixed with few microvilli belong to hairy cells, was not readily possi ble.…”
Section: Discussionmentioning
confidence: 99%
“…They are characterized by a surface richly endowed with extensive ruffles and few microvilli, that is with the surface features of both monocytes and lymphocytes. Subsequently this typi cal surface picture was demonstrated on a larger num ber of cases by different authors [10,11,16] and SEM examination suggested as an important, although complementary, diagnostic investigation in hairy cell leukemia (HCL). However, some criticism has still persisted on the reliability of SEM for diagnostic pur poses, mainly because of the potential variability of cell surface morphology.…”
Section: Introductionmentioning
confidence: 99%