Surface Plasmon Resonance-Based Immunosensor for Igm Detection with Gold Nanoparticles

Bereli, Nilay; Bakhshpour, Monireh; Topçu, Aykut Arif; Deni̇zli̇, Adil

doi:10.3390/mi12091092

Cited by 16 publications

(11 citation statements)

References 37 publications

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“…To compensate for the lack of sensitivity in detecting low-molecular weight analytes, Matsui et al have developed a gold nanoparticleembedded molecularly imprinted polymer for sensitive and selective detection of small molecules on SPR sensors [28]. Moreover, Bereli et al have utilized amine-functionalized gold nanoparticles captured by an anti-IgM-coated sensing surface of an SPR chip to perform reliable and sensitive detection of immunoglobulin M antibodies [29]. These systems represent an improvement over the standard SPR sensors in terms of sensitivity, however, they fail in part to comply with the principle of a label-free sensor due to the presence of gold nanoparticles.…”

Section: Cm-dextran Matrices For Spr Biosensorsmentioning

confidence: 99%

The Effects of Three-Dimensional Ligand Immobilization on Kinetic Measurements in Biosensors

et al. 2022

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The field of biosensing is in constant evolution, propelled by the need for sensitive, reliable platforms that provide consistent results, especially in the drug development industry, where small molecule characterization is of uttermost relevance. Kinetic characterization of small biochemicals is particularly challenging, and has required sensor developers to find solutions to compensate for the lack of sensitivity of their instruments. In this regard, surface chemistry plays a crucial role. The ligands need to be efficiently immobilized on the sensor surface, and probe distribution, maintenance of their native structure and efficient diffusion of the analyte to the surface need to be optimized. In order to enhance the signal generated by low molecular weight targets, surface plasmon resonance sensors utilize a high density of probes on the surface by employing a thick dextran matrix, resulting in a three-dimensional, multilayer distribution of molecules. Despite increasing the binding signal, this method can generate artifacts, due to the diffusion dependence of surface binding, affecting the accuracy of measured affinity constants. On the other hand, when working with planar surface chemistries, an incredibly high sensitivity is required for low molecular weight analytes, and furthermore the standard method for immobilizing single layers of molecules based on self-assembled monolayers (SAM) of epoxysilane has been demonstrated to promote protein denaturation, thus being far from ideal. Here, we will give a concise overview of the impact of tridimensional immobilization of ligands on label-free biosensors, mostly focusing on the effect of diffusion on binding affinity constants measurements. We will comment on how multilayering of probes is certainly useful in terms of increasing the sensitivity of the sensor, but can cause steric hindrance, mass transport and other diffusion effects. On the other hand, probe monolayers on epoxysilane chemistries do not undergo diffusion effect but rather other artifacts can occur due to probe distortion. Finally, a combination of tridimensional polymeric chemistry and probe monolayer is presented and reviewed, showing advantages and disadvantages over the other two approaches.

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Section: Cm-dextran Matrices For Spr Biosensorsmentioning

confidence: 99%

The Effects of Three-Dimensional Ligand Immobilization on Kinetic Measurements in Biosensors

et al. 2022

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“…Anti-Igm was fixed to the N of the AuNPs capping agent. The measurements were conducted monitoring the change in the refractive index while varying the concentration of the target Igm in solution [153].…”

Section: Biosensorsmentioning

confidence: 99%

Gold and Silver Nanoparticle-Based Colorimetric Sensors: New Trends and Applications

et al. 2021

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Gold and Silver nanoparticles (AuNPs and AgNPs) are perfect platforms for developing sensing colorimetric devices thanks to their high surface to volume ratio and distinctive optical properties, particularly sensitive to changes in the surrounding environment. These characteristics ensure high sensitivity in colorimetric devices. Au and Ag nanoparticles can be capped with suitable molecules that can act as specific analyte receptors, so highly selective sensors can be obtained. This review aims to highlight the principal strategies developed during the last decade concerning the preparation of Au and Ag nanoparticle-based colorimetric sensors, with particular attention to environmental and health monitoring applications.

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“…[11,12] SPR analyzes with the principle of reflecting the transmitted light back from two environments with different refractive indices separated by a conductive film under certain conditions and measuring this reflected light. [13,14] The refractive index on the sensor surface changes with macromolecule binding to the sensor surface. The most important advantages of SPR sensors are that the sensor chips can be used repeatedly, very low sample consumption, fast analysis time, simultaneous detection, high selectivity and sensitivity.…”

Section: Introductionmentioning

confidence: 99%

“…It can be directly and simultaneously studied without any labeling [11,12] . SPR analyzes with the principle of reflecting the transmitted light back from two environments with different refractive indices separated by a conductive film under certain conditions and measuring this reflected light [13,14] . The refractive index on the sensor surface changes with macromolecule binding to the sensor surface.…”

Section: Introductionmentioning

confidence: 99%

Testosterone Imprinted poly(HEMA‐MAA) Nanoparticles Based Surface Plasmon Resonance Sensor for Detection of Testosterone

Çimen

2022

ChemistrySelect

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The selective, sensitive, and real-time detection of testosterone was performed from both testosterone aqueous solution and artificial urine samples by using the advantages of surface plasmon resonance sensor (SPR) with molecular imprinting technique. The testosterone imprinted (MIP) and non-imprinted (NIP) nanoparticle based poly(2-hydroxyethyl methacrylatemethacrylic acid (poly(HEMA-MAA)) SPR sensors were prepared for detection of testosterone. After characterization of MIP and NIP nanoparticles, nanoparticles were attached on the SPR chip surfaces. The characterization of SPR sensor surfaces was carried out with characterization methods such as atomic force microscopy, ellipsometer and contact angle measurements. The real-time measurements for detection of testosterone were performed with testosterone imprinted SPR sensor in the linear range of 0.5-20 ng/mL concentration in testosterone aqueous solution. The imprinting factor and the limit of detection was found 4.16 and 0.049 ng/mL, respectively. The validation studies were performed with both SPR sensor and enzymelinked immunosorbent assay using testosterone samples prepared in artificial urine samples for the detection of testosterone.

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Surface Plasmon Resonance-Based Immunosensor for Igm Detection with Gold Nanoparticles

Cited by 16 publications

References 37 publications

The Effects of Three-Dimensional Ligand Immobilization on Kinetic Measurements in Biosensors

The Effects of Three-Dimensional Ligand Immobilization on Kinetic Measurements in Biosensors

Gold and Silver Nanoparticle-Based Colorimetric Sensors: New Trends and Applications

Testosterone Imprinted poly(HEMA‐MAA) Nanoparticles Based Surface Plasmon Resonance Sensor for Detection of Testosterone

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