Surface plasmon resonance characterization of monoclonal and polyclonal antibodies of malaria for biosensor applications

Sikarwar, Bhavna; Sharma, Pushpendra Kumar; Srivastava, Ambuj; Agarwal, G. S.; Boopathi, Mannan; Singh, Beer; Jaiswal, Yogesh K.

doi:10.1016/j.bios.2014.04.025

Cited by 24 publications

(10 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In comparison to 'gold standard' microscopy, the immunosensor exhibited a sensitivity of 92% and a specificity of 90%. In another study that detected monoclonal antibodies to recombinant PfHRP-2 (MoaPfHRP-2), a gold chip was pre-treated with 4-mercaptobenzoic to immobilize recombinant PfHRP-2, then monitored for interactions between the antigen and antibody [68]. Label free surface plasmon resonance (SPR) screening of the interaction between the recombinant protein and target antibody produced a LoD of 5.6 pg (Table 2).…”

Section: Detection Of Pfhrp-2 In Clinical Samplesmentioning

confidence: 99%

Recent Advances in the Development of Biosensors for Malaria Diagnosis

Krampa

Aniweh

Kanyong

et al. 2020

Sensors

View full text Add to dashboard Cite

The impact of malaria on global health has continually prompted the need to develop more effective diagnostic strategies that could overcome deficiencies in accurate and early detection. In this review, we examine the various biosensor-based methods for malaria diagnostic biomarkers, namely; Plasmodium falciparum histidine-rich protein 2 (PfHRP-2), parasite lactate dehydrogenase (pLDH), aldolase, glutamate dehydrogenase (GDH), and the biocrystal hemozoin. The models that demonstrate a potential for field application have been discussed, looking at the fabrication and analytical performance characteristics, including (but not exclusively limited to): response time, sensitivity, detection limit, linear range, and storage stability, which are first summarized in a tabular form and then described in detail. The conclusion summarizes the state-of-the-art technologies applied in the field, the current challenges and the emerging prospects for malaria biosensors.Sensors 2020, 20, 799 2 of 21 emergence and spread of drug resistance. It could also reduce the pool of individuals who can contribute to malaria transmission [5].To date, many technologies have attempted to circumvent the challenges in malaria diagnostics with technologies that address point-of-care needs and early stage asymptomatic detection. In this review, we first comprehensively summarize the biomarkers targeted during the course of malaria with emphasis on the importance of sensitive early detection. Next, we provide an overview of the recent advances in biosensor technologies for the detection of the most targeted biomarkers, focusing on: development, analytical performances, and suitability for point of care testing. The prevailing challenges and future outlook of the use of these technologies in the field are also highlighted. Parasite Development in Humans, Biomarkers, and DiagnosisThe developmental cycle of Plasmodium species that infect humans is briefly illustrated in Figure 1 [6]. The cycle begins with the injection of sporozoites into the host's circulation by an infected female Anopheles mosquito. The sporozoites then target and enter hepatocytes where they multiply and differentiate into merozoites. This stage of the parasite life cycle is known as pre-erythrocytic. In infections involving P. vivax and P. ovale, dormant forms of the liver stage, called hypnozoites may persist in the liver [7] and cause relapse of the infection, thereby making it difficult to eradicate. The pre-erythrocytic stage is essential in the establishment of malaria infection. This stage is asymptomatic, however, it is difficult for diagnostic tools to detect sporozoites because hepatocytes invasion occurs within 30-45 min after sporozoites are inoculated by the infected mosquito [8,9]. This short time and low numbers of sporozoites injected leaves little time for their detection. Some efforts in finding biomarkers for detection of early liver stage or the dormant form have identified the Plasmodium liver-specific protein 2 (LISP2) [10,11]. The sporozoite surface circum...

show abstract

Section: Detection Of Pfhrp-2 In Clinical Samplesmentioning

confidence: 99%

Recent Advances in the Development of Biosensors for Malaria Diagnosis

Krampa

Aniweh

Kanyong

et al. 2020

Sensors

View full text Add to dashboard Cite

show abstract

“…The drawbacks of these techniques are that they are either of low sensitivity, which could lead to inappropriately withholding treatment from patients with malaria [ 19 ], or are as time consuming as the lab-based methods. Recent literature has reported the development of different sensors for malaria based on biomarker and antibody detection [ 24 , 25 ]. Therefore, in this work, we investigated the development of a rapid and highly sensitive sensor based on a screen-printed device, which would enable its use in low-resource countries.…”

Section: Introductionmentioning

confidence: 99%

Development of an Immunosensor for PfHRP 2 as a Biomarker for Malaria Detection

2017

View full text Add to dashboard Cite

Plasmodium falciparum histidine-rich protein 2 (PfHRP 2) was selected in this work as the biomarker for the detection and diagnosis of malaria. An enzyme-linked immunosorbent assay (ELISA) was first developed to evaluate the immunoreagent’s suitability for the sensor’s development. A gold-based sensor with an integrated counter and an Ag/AgCl reference electrode was first selected and characterised and then used to develop the immunosensor for PfHRP 2, which enables a low cost, easy to use, and sensitive biosensor for malaria diagnosis. The sensor was applied to immobilise the anti-PfHRP 2 monoclonal antibody as the capture receptor. A sandwich ELISA assay format was constructed using horseradish peroxidase (HRP) as the enzyme label, and the electrochemical signal was generated using a 3, 3′, 5, 5′tetramethyl-benzidine dihydrochloride (TMB)/H2O2 system. The performance of the assay and the sensor were optimised and characterised, achieving a PfHRP 2 limit of detection (LOD) of 2.14 ng·mL−1 in buffer samples and 2.95 ng∙mL−1 in 100% spiked serum samples. The assay signal was then amplified using gold nanoparticles conjugated detection antibody-enzyme and a detection limit of 36 pg∙mL−1 was achieved in buffer samples and 40 pg∙mL−1 in serum samples. This sensor format is ideal for malaria detection and on-site analysis as a point-of-care device (POC) in resource-limited settings where the implementation of malaria diagnostics is essential in control and elimination efforts.

show abstract

“…Waddington et al . demonstrated that the KD value of FX-Ad interaction was approximately 1.8 × 10 −9 M 24 , although the KD value of antigen-antibody interaction is generally reported as 10 −8 -10 −10 M 34 , 35 . These data indicated that the affinity of FX-Ad interaction was comparable to that of antigen-antibody interaction and that FX-Ad interaction was stable in the blood.…”

Section: Discussionmentioning

confidence: 99%

Antibodies against adenovirus fiber and penton base proteins inhibit adenovirus vector-mediated transduction in the liver following systemic administration

Tomita

Sakurai

Iizuka

et al. 2018

Sci Rep

View full text Add to dashboard Cite

Pre-existing anti-adenovirus (Ad) neutralizing antibodies (AdNAbs) are a major barrier in clinical gene therapy using Ad vectors and oncolytic Ads; however, it has not been fully elucidated which Ad capsid protein-specific antibodies are involved in AdNAb-mediated inhibition of Ad infection in vivo. In this study, mice possessing antibodies specific for each Ad capsid protein were prepared by intramuscular electroporation of each Ad capsid protein-expressing plasmid. Ad vector-mediated hepatic transduction was efficiently inhibited by more than 100-fold in mice immunized with a fiber protein-expressing plasmid or a penton base-expressing plasmid. An Ad vector pre-coated with FX before administration mediated more than 100-fold lower transduction efficiencies in the liver of warfarinized mice immunized with a fiber protein-expressing plasmid or a penton base-expressing plasmid, compared with those in the liver of warfarinized non-immunized mice. These data suggest that anti-fiber protein and anti-penton base antibodies bind to an Ad vector even though FX has already bound to the hexon, and inhibit Ad vector-mediated transduction. This study provides important clues for the development of a novel Ad vector that can circumvent inhibition with AdNAbs.

show abstract

Surface plasmon resonance characterization of monoclonal and polyclonal antibodies of malaria for biosensor applications

Cited by 24 publications

References 50 publications

Recent Advances in the Development of Biosensors for Malaria Diagnosis

Recent Advances in the Development of Biosensors for Malaria Diagnosis

Development of an Immunosensor for PfHRP 2 as a Biomarker for Malaria Detection

Antibodies against adenovirus fiber and penton base proteins inhibit adenovirus vector-mediated transduction in the liver following systemic administration

Contact Info

Product

Resources

About