Surface plasmon resonance detection of E. coli and methicillin-resistant S. aureus using bacteriophages

Development of a Simultaneous Detection Method for Foodborne Pathogens Using Surface Plasmon Resonance Biosensors

“…1) (Rich and Myszka, 2001). SPR biosensors have been used by many research groups for the detection of foodborne pathogens (Meeusen et al, 2005;Oh et al, 2004;Subramanian et al, 2006;Tawil et al, 2012;Waswa et al, 2007;Wei et al, 2007). However, all these studies detected only one species of target pathogen in one detection period.…”

Plasmonic imaging of protein interactions with single bacterial cells

“…Coli O157:H7 by direct detection of bacterial cells binding to surface (Tawil et al, 2012;Torun et al, 2012) , indirect detection of surface immobilized cells using complementary probes (Medina et al, 1997;Subramanian et al, 2006) or coupling SPR with other techniques. (Zordan et al, 2009) In this study, we focus on the binding kinetics of goat anti--E Coli O157:H7 IgG polyclonal antibody(Ab157) (Medina et al, 1997;Subramanian et al, 2006) onto single E. Coli O157:H7 cells.…”

The Detection Method ofEscherichia coliin Water Resources: A Review

“…The demand for high throughput label-free multiplexed biosensors for biological sensing has increased in the last decades. Therefore, the circumstances have led to the development of fiber optics [38][39][40][41], planar waveguides [42][43][44] and surface plasmon resonance [45][46][47][48][49][50] in the area of optical biosensor. These techniques are modified by the interaction of bio-recognition element, such as enzymes, antibodies, antigens, or receptors of E. coli used on it to make it highly selective.…”

“…In planar optical waveguides, thin optically transparent film with that has high refractive more than superstrate mediums and adjacent substrate is usually being used. Under this condition, light is combined into the thin waveguide film are constrained to the layer and generate to meaningful distances as shown in Figure 4 [47]. SPR is known as label-free biosensor that produce real-time result based on changes the refractive index on the surface of sensor part which is proportional to the biomolecule concentration.…”

“…Colquhoun et al has utilized a medium of trimethylamine N-oxide (TMAO) and glucuronic acid that are very specific for E. coli in imperometric based sensor to detect E. coli in water samples [47]. Promising result had shown in the research which indicates the change of impedance during the growing of E. coli bacteria.…”