2008
DOI: 10.1364/oe.16.020734
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Surface plasmon resonance imaging of cell-substrate contacts with radially polarized beams

Abstract: We demonstrate the proof-of-concept for surface plasmon resonance sensing and imaging via a virtual probe at the cell-substrate interface of a biological cell in aqueous media. The technique is based on the optical excitation by focused radially polarized beams of localized surface plasmons, which forms a virtual probe on the metal substrate. The intensity distribution at the back focal plane of the objective lens enables quantitative measurements to be made of the cell-substrate contact. The acquired data is … Show more

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Cited by 85 publications
(45 citation statements)
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“…As demonstrations, we first examine the SPP patterns produced by focusing a cylindrical beam into a Kretschmann-type structure of a thin metal film deposited on a glass plate. For a radially polarized cylindrical beam22, the detected SPP pattern shows a center spot of 0.355 λ 0 , which agrees well with theory. Following the same methodology, we visualize directly nano-focusing and interferences of surface plasmon waves created by a particular class of plasmonic near-field lenses, further demonstrating the universal use of the developed near-field imaging method for more general evanescent wave characterizations at subwavelength scales.…”
supporting
confidence: 85%
“…As demonstrations, we first examine the SPP patterns produced by focusing a cylindrical beam into a Kretschmann-type structure of a thin metal film deposited on a glass plate. For a radially polarized cylindrical beam22, the detected SPP pattern shows a center spot of 0.355 λ 0 , which agrees well with theory. Following the same methodology, we visualize directly nano-focusing and interferences of surface plasmon waves created by a particular class of plasmonic near-field lenses, further demonstrating the universal use of the developed near-field imaging method for more general evanescent wave characterizations at subwavelength scales.…”
supporting
confidence: 85%
“…Many authors have advocated the use of radial polarisation in SP microscopy (Moh et al, 2008) and indeed we can see from Eq. (1) that this allows all the incident light to be ppolarised so that the magnitude of the V(z) oscillations is increased.…”
Section: Interferometric Microscopy Methods For Determination Of Locamentioning
confidence: 89%
“…In this article, we report a variant of the plasmonic tweezers for trapping and manipulating metallic particles that is based on a highly focused plasmonic field on a flat metal film excited by a radially polarized (RP) beam (also called plasmonic virtual probe232425). This variant can attract and trap both Rayleigh- and Mie-type metallic particles.…”
mentioning
confidence: 99%