Surface plasmon resonance imaging of cells and surface-associated fibronectin

Abstract

Background: A critical challenge in cell biology is quantifying the interactions of cells with their extracellular matrix (ECM) environment and the active remodeling by cells of their ECM. Fluorescence microscopy is a commonly employed technique for examining cell-matrix interactions. A label-free imaging method would provide an alternative that would eliminate the requirement of transfected cells and modified biological molecules, and if collected nondestructively, would allow long term observation and analysis of live cells.

Preliminary approach of real‐time monitoring in vitro matrix mineralization based on surface plasmon resonance detection

“…Also, these properties enable SPR sensors to detect cell activities because cellular metabolism is accompanied by a small change in the membrane-localized refractive index (Kim et al, 2008;Zhang et al, 2009). However, to our knowledge, long-term and continuous SPR measurement of live cells has not been reported yet, while 1-day observation of the protein deposition occurring at vascular muscle cells was reported (Peterson et al, 2009).…”

“…In principle, SPR biosensors measure a refractive index change at a dielectric-metal interface. SPR technique is quantitative, sensitive, fast, and label-free and thus, it has been used to detect the kinetics of surface-immobilized biomolecules (Kim et al, 2009;Peterson et al, 2009). Also, these properties enable SPR sensors to detect cell activities because cellular metabolism is accompanied by a small change in the membrane-localized refractive index (Kim et al, 2008;Zhang et al, 2009).…”

Advances in surface plasmon resonance-based high throughput biochips

“…To date, the information extraction from most SPRbased sensors (or micro-arrays) is based on the response of resonance angle or reflected intensity to the target molecules [41][42][43][44][45][46], that is to say, the anchoring of biomolecules gives a small variation of the SPR angle, or the reflected intensity at a fixed angle adjacent to SPR angle. As illustrated in Fig.…”

High frame rate multi-resonance imaging refractometry with distributed feedback dye laser sensor

“…2,3 Several optical sensors allow spatially resolving the refractive index on surfaces in microscope like set-ups. This imaging refractometry has e.g., been applied to detect nanoparticles, 4,5 proteins, [6][7][8] viruses [9][10][11] and cells [12][13][14][15][16] and has enabled imaging as well as multiplexed sensing. 10,17 In this context, label-free signal transduction is based on surface plasmon resonance, 9,12,13,17,18 plasmonic hole arrays, [19][20][21][22][23] photonic crystals 5,[14][15][16] and interferometry.…”