Surface plasmon resonance imaging system with Mach-Zehnder phase-shift interferometry for DNA micro-array hybridization

Abstract

Surface plasmon resonance (SPR) imaging system is presented as a novel technique based on modified Mach-Zehnder phase-shifting interferometry (PSI) for biomolecular interaction analysis (BIA), which measures the spatial phase variation of a resonantly reflected light in biomolecular interaction. In this technique, the micro-array SPR biosensors with over a thousand probe NDA spots can be detected simultaneously. Owing to the feasible and swift measurements, the micro-array SPR biosensors can be extensively applied to the nonspecific adsorption of protein, the membrane/protein interactions, and DNA hybridization. The detection sensitivity of the SPR PSI imaging system is improved to about 1 pg/mm 2 for each spot over the conventional SPR imaging systems. The SPR PSI imaging system and its SPR sensors have been successfully used to observe slightly index change in consequence of argon gas flow through the nitrogen in real time, with high sensitivity, and at high-throughout screening rates.

Surface plasmon resonance phase-shift interferometry: Real-time DNA microarray hybridization analysis

“…13,14 Several SPR interferometers have been developed to measure the spatial phase variation associated with SPR, but the systemic modular and integration and fringe analysis remain problematic. [15][16][17][18] Consequently, this study proposes a novel SPR imaging system based on modified Mach-Zehnder phase-shifting interferometry ͑PSI͒ in which the spatial phase variation of a resonantly reflected light is measured in order to observe DNA microarray hybridization. It is shown that the detection limit of the developed SPR-PSI imaging system is improved to approximately 2.5ϫ10 Ϫ7 refraction index change at each individual spot, which represents a resolution improvement of 1-2 orders compared to that of conventional SPR imaging systems.…”

A common-path phase-shift interferometry surface plasmon imaging system

“…63351; Fax: +886- 6-2766549. produces the best resolution [3]. Hence, we had developed a SPR phase imaging system for high-throughput real-time dynamic measurement of biomolecular interaction causing slight variation in the dielectric constant or thickness of biomolecular material [10]. However, this system like other SPR phase imaging systems can not match the strict demand of real-time kinetic study for BIA because it lacks long-term stability [6][7][8].…”

“…It has been widely applied to biomolecular interaction analysis (BIA) [3]. Currently, in addition to convenience, economy, and fast speed, developing SPR technology for high sensitivity, high resolution, and reliable imaging capability would make high-throughput biomolecular screening possible [3][4][5][6][7][8][9][10].…”

“…With the multilayer simulation plots, it can be shown that the reflectivity of P-wave drops drastically and the phase jumps at the resonance angle while S-wave does not excite SPW and therefore does not demonstrate absorption dip and drastic phase variation in its reflection[10].Proc. of SPIE Vol.…”

Surface plasmon resonance phase-shift interferometry: real-time microarray biomolecular interaction analysis

“…Even if these SPR imaging systems are capable of high-throughput screening, the detection resolution is hard to detect low concentrations of low molecular weight analytes [7][8][9][10] . Therefore, interferometry is demonstrated to measure the spatial phase variation under SPR [11][12][13][14] . In this paper, a novel SPR imaging system based on modified Mach-Zehnder phase-shifting interferometry (PSI) that measures the spatial phase variation of a resonantly reflected light to observe DNA microarray hybridization is demonstrated.…”