Surface plasmon resonance immunosensor for bacteria detection

Baccar, Hamdi; Mejri, M. B.; Hafaiedh, I.; Ktari, T.; Aouni, Mahjoub; Abdelghani, Adnane

doi:10.1016/j.talanta.2010.05.060

Cited by 83 publications

(42 citation statements)

References 6 publications

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“…Analyte binding to the transducer surface changes its refractive index, which in turn alters the angle of light exiting the prism (the SPR angle). Various SPR-based biosensors have been developed for the detection of whole bacterial cells using a variety of bioreceptors, including antibodies (39,40), bacteriophages (31,41), and lectins (29,42).…”

Section: Optical Biosensorsmentioning

confidence: 99%

Biosensors for Whole-Cell Bacterial Detection

et al. 2014

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SUMMARY Bacterial pathogens are important targets for detection and identification in medicine, food safety, public health, and security. Bacterial infection is a common cause of morbidity and mortality worldwide. In spite of the availability of antibiotics, these infections are often misdiagnosed or there is an unacceptable delay in diagnosis. Current methods of bacterial detection rely upon laboratory-based techniques such as cell culture, microscopic analysis, and biochemical assays. These procedures are time-consuming and costly and require specialist equipment and trained users. Portable stand-alone biosensors can facilitate rapid detection and diagnosis at the point of care. Biosensors will be particularly useful where a clear diagnosis informs treatment, in critical illness (e.g., meningitis) or to prevent further disease spread (e.g., in case of food-borne pathogens or sexually transmitted diseases). Detection of bacteria is also becoming increasingly important in antibioterrorism measures (e.g., anthrax detection). In this review, we discuss recent progress in the use of biosensors for the detection of whole bacterial cells for sensitive and earlier identification of bacteria without the need for sample processing. There is a particular focus on electrochemical biosensors, especially impedance-based systems, as these present key advantages in terms of ease of miniaturization, lack of reagents, sensitivity, and low cost.

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Section: Optical Biosensorsmentioning

confidence: 99%

Biosensors for Whole-Cell Bacterial Detection

et al. 2014

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“…Nevertheless, the detection limits achieved with Matrix-Lysis and qPCR are as good as the quantification limits associated with high-technology methods, such as fiber optic immunosensors coupled with immunomagnetic separation or antibody-based immunosensors [29,30]. To our knowledge, Matrix-Lysis is the only economical and easy-to-use tissue preparation method that can compete with these novel high-technology methods, considering the LOD and coefficients of determination in whole dilution series.…”

Section: Discussionmentioning

confidence: 99%

Quantification of Gram-positive bacteria: adaptation and evaluation of a preparation strategy using high amounts of clinical tissue

et al. 2014

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BackgroundA preparation method for quantification of bacteria in tissues is obligatory to reduce tissue mass, concentrate the target, purify, remove inhibitory substances and to achieve constant target recovery rates. No preparation method has been available until now for a high mass of tissue applicable for routine use and analytical veterinary diagnostics.ResultsThis study describes an easy-to-use tissue preparation protocol to quantify Gram-positive bacteria from a large volume of tissue matrix. A previously published sample preparation method (Matrix-Lysis) from food science was successfully adapted for clinical use on tissues from pigs, including cerebrum, spinal cord, lung, liver, ileum, colon, caecum, kidney and muscle tissue. This tissue preparation method now permits quantification of pathogens from 5 g of organic matrix, which is a 20–200 fold increase by weight compared to other methods. It is based on solubilization of the sample matrix with either a chaotrope plus detergent or divalent salts as solubilization agents. The method was designed as a modular system, offering the possibility to change lysis buffers, according to tissue solubilization characteristics and the intended detection method (molecular or culture). Using Listeria monocytogenes as model organism, viable cell quantification or DNA extraction and quantitative real-time PCR were performed after Matrix-Lysis to determine recovery rates and detection limit (LOD). The adapted Matrix-Lysis protocol resulted in high recovery rates (mean value: 76% ± 39%) for all tested organs, except kidney, and recovery was constant over 5 log scales for all tested buffer systems. The LOD for Matrix-Lysis with subsequent plate count method (PCM) was as low as 1 CFU/5 g, while for qPCR based detection the LOD was 102 bacterial cell equivalents (BCE)/5 g for two buffer systems.ConclusionsThis tissue preparation is inexpensive and can be easily used for routine and analytical veterinary diagnostics. Inoculation studies or hazard assessments can profit from this tissue preparation method and it is anticipated that this study will be a valuable source for further research on tissue preparation strategies.

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“…The SPR signal was recorded upon injection of CRP-antigen. More details on SPR phenomenon can be found in reference [9]. …”

Section: Spr Instrumentationmentioning

confidence: 99%

Surface Plasmon Resonance for C-Reactive Protein Detection in Human Plasma

Chammem¹,

Hafaid²,

Meilhac³

et al. 2014

JBNB

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In this work, we describe an approach of detecting biomarkers by Surface Plasmon Resonance imaging (SPRi) technique in real samples. Two C-Reactive Protein (CRP)-antibody immobilization methods were used: The first method was based on direct physisorption of CRP-antibody onto gold surface; the second one was based on oriented CRP-antibody with protein G intermediate layer.The two developed immunosensors were tested against CRP antigen in phosphate buffer saline solution with the SPRi technique. The response of the developed immunosensors was reproducible and stable. The detection limit of 10 pg•mL −1 and 50 pg•mL −1 CRP-antigen was observed with and without protein G respectively with this technique. Moreover, the developed SPRi immunosensor was used for CRP-antigen detection in human plasma. A detection limit of 5 ng•mL −1 and 10 ng•mL −1 was obtained with and without protein G respectively. These obtained results were compared to those obtained with QCM (Quartz Crystal Microbalance) and Enzyme-Linked Immunosorbent Assay (ELISA) techniques.

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Surface plasmon resonance immunosensor for bacteria detection

Cited by 83 publications

References 6 publications

Biosensors for Whole-Cell Bacterial Detection

Biosensors for Whole-Cell Bacterial Detection

Quantification of Gram-positive bacteria: adaptation and evaluation of a preparation strategy using high amounts of clinical tissue

Surface Plasmon Resonance for C-Reactive Protein Detection in Human Plasma

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