Surface plasmon resonance immunosensor using Au nanoparticle for detection of TNT

Kawaguchi, Toshikazu; Shankaran, Dhesingh Ravi; Kim, Sook Jin; Matsumoto, Kiyoshi; Toko, Kiyoshi; Miura, Norio

doi:10.1016/j.snb.2008.03.005

Cited by 68 publications

(48 citation statements)

References 29 publications

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“…Prepared sensor surfaces were used for sensing immediately after the fabrication process. These sensor surfaces should be very stable, because their fabrication involved only covalent bonds (S-Au, S-S, and NHCO) [35].…”

Section: Spr Sensingmentioning

confidence: 99%

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Effect of alkanethiol molecular structure on sensitivity of surface plasmon resonance sensor

Suherman

Morita

Kawaguchi

2015

Sensors and Actuators B: Chemical

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This work aims to find the key factor determining the detection limit for an indirect competitive inhibition immunoassay using surface plasmon resonance (SPR) sensing. In our previous work, the thiol solution concentration used in a self-assembly process highly affected the alkanethiol monolayer structure on the sensor surface [Suherman et al., 2014]. It was noticed that the monolayer structure determined the immunoassay sensitivity due to the orientation and the surface concentration of antigen in domain structure of monolayer. To study the effect of orientation of antigen, we examined here three types of alkanethiol compounds: dithiobis(succinimidyl undecanoate) (DSU; straight-chain alkanethiol), carboxy-EG 6 -undecanethiol (CEG 6 ; flexible-chain alkanethiol), and(C 2 -NTA; three-branched alkanethiol). By electrochemical reductive desorption, the surface concentration of DSU, CEG 6 , and C 2 -NTA were estimated to be 6.6 × 10 -10 , 8.5 × 10 -10 , 4.6 × 10 -10 mol cm -2 , respectively. Subsequently, SPR suggested that the ratios of immobilized antigen (clenbuterol) per thiol were 0.13, 0.16, and 0.15 for DSU, CEG 6 , and C 2 -NTA, respectively. This suggests that the amount of immobilized clenbuterol was defined by the molecular size of clenbuterol. Sensor surface structures on a molecular scale were evaluated using high-resolution scanning tunneling microscopy (STM), which showed tilted clenbuterol with θ DSU > θ CEG6 > θ C2-NTA . For SPR sensing of clenbuterol, C 2 -NTA showed the highest sensitivity (LOD = 10 ppt) among the examined alkanethiols, because clenbuterol formed a laid-on structure. Based on kinetics, the key factor for sensing performance is discussed.2

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Section: Spr Sensingmentioning

confidence: 99%

“…For detecting clenbuterol, the indirect competitive inhibition immunoassay technique was employed [13,[35][36][37]. In this study, the antibody solution (PBS solution containing 1 µg mL -1 of Ab) was premixed with a sample solution (PBS solution containing clenbuterol) before injection into the sensing system.…”

Section: Immunoassaysmentioning

confidence: 99%

Effect of alkanethiol molecular structure on sensitivity of surface plasmon resonance sensor

Suherman

Morita

Kawaguchi

2015

Sensors and Actuators B: Chemical

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“…Indirect competitive inhibition method allowed the middle-sized SPR sensor to detect trinitrotoluene (TNT) at ppt level [64]. However, this enhanced SPR signal was not high enough to detect TNT at ppt level by a miniaturized SPR sensor.…”

Section: Application Of Spr Nano-metalmentioning

confidence: 99%

“…TNT detectable range of this immune surface was from 10 ppt (10 pg/ml) to 100 ppb (100 ng/ml), which was almost comparable to that without Au nanoparticle. The observed resonance angle change due to binding monoclonal TNT antibody (M-TNT Ab) with the immune surface modified with Au nanoparticlewas amplified to four times higher than that in absence of Au nanoparticle [64].…”

Section: Application Of Spr Nano-metalmentioning

confidence: 99%

Application of Surface Plasmon Resonance Based on a Metal Nanoparticle

Sadrolhosseini¹,

Noor²,

Moksin³

2012

Plasmonics - Principles and Applications

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“…[19][20][21] Chitosan is a high-molecular biopolymer with many amine groups bearing high affinity to Au nanoparticles (NPs) with unique surface plasmon resonance. [22][23][24][25] Hence, chitosan nanoparticles can be employed as templates which can anchor Au NPs and grow into gold layer. The resultant chitosan-gold composites can be used as a biocompatible SERS substrate which can enhance the Raman signals of probe molecules.…”

Section: Introductionmentioning

confidence: 99%

Fabrication of Chitosan-gold Nanocomposites Combined with Optical Fiber as SERS Substrates to Detect Dopamine Molecules

Lim¹,

Kang²

2014

Bulletin of the Korean Chemical Society

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This research was aimed to fabricate an optical fiber-based SERS substrate which can detect dopamine neurotransmitters. Chitosan nanoparticles (NPs) were firstly anchored on the surface of optical fiber, and then gold layer was subsequently deposited on the anchored chitosan NPs via electroless plating method. Finally, chitosan-gold nanocomposites combined with optical fiber reacted with dopamine molecules of 100-1500 mg/ day which is a standard daily dose for Parkinson's disease patients. The amplified Raman signal at 1348 cm 1 obtained from optical fiber-based SERS substrate was plotted versus dopamine concentrations (1-10 mM), demonstrating an approximate linearity of Y = 303.03X + 2385.8 (R 2 = 0.97) with narrow margin errors. The optical fiber-based Raman system can be potentially applicable to in-vitro (or in-vivo) detection of probe molecules.

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Surface plasmon resonance immunosensor using Au nanoparticle for detection of TNT

Cited by 68 publications

References 29 publications

Effect of alkanethiol molecular structure on sensitivity of surface plasmon resonance sensor

Effect of alkanethiol molecular structure on sensitivity of surface plasmon resonance sensor

Application of Surface Plasmon Resonance Based on a Metal Nanoparticle

Fabrication of Chitosan-gold Nanocomposites Combined with Optical Fiber as SERS Substrates to Detect Dopamine Molecules

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