Surface Plasmon Resonance Sensors

Oliveira, Leiva Casemiro; Lima, Antonio Marcus Nogueira; Thirstrup, Carsten; Neff, H.

doi:10.1007/978-3-030-17486-6

Cited by 23 publications

(9 citation statements)

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“…As a next step, we decided to independently confirm the interaction between selected intratumoral Ig and their putative target antigens by using surface plasmon resonance (SPR) 15 . For each intratumoral Ig sequence, we characterized the binding affinity of the corresponding recombinant antibody to its putative target antigen, having sourced the antigen independently of the vendor used for the high-throughput proteomics screening.…”

Section: Resultsmentioning

confidence: 99%

The landscape of high-affinity human antibodies against intratumoral antigens

Rakočević¹,

Gotlova²,

Santiago³

et al. 2021

Preprint

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Intratumoral expression of antibody transcripts can be detected across different cancer types and is correlated with positive clinical outcomes. However, the antigens recognized by these antibodies remain largely unknown. Here we inferred the paired sequence of thousands of clonally expanded antibodies using bulk RNA sequencing data from solid tumors in The Cancer Genome Atlas (TCGA). After expressing 283 candidate antibodies in mammalian cells, we individually tested them against a library of twenty thousands full-length human proteins and a library of five thousands membrane-only proteins. Using surface plasmon resonance we confirmed 13 high-affinity antibodies that bind to their targets with KD in the low nanomolar range. Our work provides insights into the antigens that drive B cell responses in human cancers while also directly identifying fully human, high-affinity antibodies against them.

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Section: Resultsmentioning

confidence: 99%

The landscape of high-affinity human antibodies against intratumoral antigens

Rakočević¹,

Gotlova²,

Santiago³

et al. 2021

Preprint

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“…A SP is a collective oscillation of conduction electrons occurring at the interface between the surface of a metal film like gold, silver, or other appropriate conducting materials [17], and a dielectric medium above, which may be a bio-/ chemical affinity layer, a fluid, or an electrolyte solution. At an appropriate wavelength and angle of incidence to the metal film, p -polarized light may couple resonantly with an SP, forming an SPP.…”

Section: Measurement Configurationmentioning

confidence: 99%

Ion-specific quantitative measurement scheme using transit-time surface plasmon resonance

Thirstrup

Deleebeeck

Lima

2019

Meas. Sci. Technol.

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A sensitive, ion-specific measurement scheme is proposed and analysed theoretically. It is based on transit-time surface plasmon resonance (SPR) sensing in an electrolytic conductivity cell comprising an electrode supporting surface plasmons (SPs). The transit-time SPR response involves dynamics of ion transport in the electrolytic conductivity cell and a highly sensitive SPR response from the electrode supporting SPs. This is modelled by solving the modified-Poisson–Nernst–Planck equations and the Fresnel equations. It is shown that the magnitude of the transit-time SPR detection signal scales with the square root of ion concentration (), whilst bulk SPR detection scales linearly with ion concentration (). Transit-time SPR is, therefore, a superior detection scheme at low ion concentrations. Analyses by Tikhonov regularization show that transit-time SPR response enables specific ion detection through differences in ion mobility and charge. The proposed method is purely physical, not requiring chemical affinity layers or chemical reduction-oxidation processes at the electrodes. It is based on a differential measurement scheme with and without voltage applied. Compared to conductivity sensors, the method adds specificity, while compared to bulk SPR sensors, it improves the resolution at low concentration levels and enables specificity without use of surface chemistry or biochemistry.

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“…For asymmetric dips in the SPR reflectivity curves (where the dip does not correspond to the R = 0 value), FWHM can be defined as the full width at half minima reflectivity points [19]. FOM is the ratio of the sensitivity to the FWHM [17], i.e., FOM = S FWHM . The SNR is defined as the ratio of the reflectivity dip (with reference to R = 1 level) and FWHM, i.e.…”

Section: Introductionmentioning

confidence: 99%

“…SNR = dip FWHM . A high value of SNR is desirable especially if the sensor is to be used for the detection of very low concentration/size of the chemical/bio-molecule entities present in the analyte [15]- [17], [20].…”

Section: Introductionmentioning

confidence: 99%

Enhancement of Optical Coupling Efficiency of Surface Plasmon Resonance Based Sensors

2022

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The optical coupling efficiency of surface plasmon resonance (SPR) based sensors is theoretically investigated from the first principle. Analytical expressions for the instantaneous electric flux lines associated with the TM modes of a three-layer dielectric-metal-dielectric (DMD) waveguide structure are calculated. It is shown that these electric flux lines appear in the form of dis-continuous loops at the metaldielectric interfaces. A careful investigation of the electric flux loops reveals that the transverse component of the electric flux dominates at the interface, and away from the interface the longitudinal electric flux component takes over. It is hence shown that in the incident p-polarized optical light which is used to excite the surface plasmon (SP) modes in SPR sensors if the transverse component of the electric field is increased then a significant increase in the input optical coupling efficiency is observed. The increased input optical coupling increases the real part of the effective index of the SP mode of the sensor yielding an increased sensitivity of the SPR sensor. Calculations are done to optimize various design parameters of an SPR sensor in a typical prism coupling Kretschmann configuration in an angle interrogation setup. It is shown that there is an increase of 64.33% per RIU in optical coupling efficiency with a decrease in the refractive index of the high index input coupling prism and an increase of 58.787% per RIU with an increase in the refractive index of the affinity layer. Enhancement of optical coupling efficiency due to the thickness of the metal film as well as the wavelength of the input optical light has also been evaluated. The corresponding improvement in the sensitivity and changes in full width at half maxima (FWHM), the figure of merit (FOM), and signal to noise ratio (SNR) of the SPR sensor are also reported.INDEX TERMS Electric and magnetic flux loops in optical waveguide modes, evanescent waves, optical coupling efficiency, surface plasmon resonance-based sensors, sensitivity, figure of merit.

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Surface Plasmon Resonance Sensors

Cited by 23 publications

References 0 publications

The landscape of high-affinity human antibodies against intratumoral antigens

The landscape of high-affinity human antibodies against intratumoral antigens

Ion-specific quantitative measurement scheme using transit-time surface plasmon resonance

Enhancement of Optical Coupling Efficiency of Surface Plasmon Resonance Based Sensors

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