Surface plasmone resonance sensor for biomimetic detection of progesterone with macroporous molecularly imprinted polymers prepared by visible light

Abstract

Reversible addition-fragmentation chain transfer (RAFT) polymerization mechanism was used for the preparation of molecularly imprinted polymers (MIPs) film using 4-cyano-4-[(dodecylsulfanylthiocarbonyl)sulfanyl]pentanoic acid (CDTPA) as chain transfer reagent and visible light initiator. In addition, the effect of CDTPA concentration on the polymerization rate was monitored, establishing that too high CDTPA concentration would inhibit the polymerization rate. The MIPs film was characterized by contact angle measurement, frontier transfer infrared spectroscopy (FTIR) and scanning electron microscope (SEM) which showed the successful grafting of MIPs films onto surface plasmon resonance (SPR) sensor chip. The response of progesterone sensing ranged from 10−16 to 10−6mol/L with low detection limit of 1× 10−16mol/L in PBS buffer (pH 7.4) and the MIPs film exhibited good selectivity, reproducibility and stability. Moreover, the sensors had been successfully applied to detect progesterone in tap water, lake water and human urine turning out with wide detection range and low detection limit.

Electrochemical immunosensor for detection of Plasmodium vivax lactate dehydrogenase

“…After SAMs formation, a noticeable decrease in CA values was observed. Similar behavior has been reported by other authors (25,26) and can be explained by terminal amino groups in the cysteamine structure attributing greater hydrophilicity to the surface.…”

Cardiac Troponin I-Responsive Nanocomposite Materials for Voltammetric Monitoring of Acute Myocardial Infarction

“…However, upon adsorption of the Cys-epitope, the gold surface exhibited a significant change in static angle, becoming highly hydrophilic (8.95°), as shown in Figure B. This increase in hydrophilicity is likely due to the assembly of cysteine’s –SH groups on the gold surface, forming a uniform layer of Au–S bonds . Also, the templated surface showed no fluorescence properties since the peptide lacked a fluorophore moiety.…”

“…This increase in hydrophilicity is likely due to the assembly of cysteine’s –SH groups on the gold surface, forming a uniform layer of Au–S bonds. 41 Also, the templated surface showed no fluorescence properties since the peptide lacked a fluorophore moiety. When comparing the contact angles of AP-GQD-AuNP-MIP and AP-GQD-AuNP-NIP films, it was observed that the former (30.1°) exhibited higher hydrophilicity than the latter film (40.4°).…”

“…This increase in hydrophilicity is likely due to the assembly of cysteine's −SH groups on the gold surface, forming a uniform layer of Au−S bonds. 41 Also, the templated surface showed no fluorescence properties since the peptide lacked a fluorophore moiety. When comparing the contact angles of AP-GQD-AuNP-MIP and AP-GQD-AuNP-NIP films, it was observed that the former (30.1°) exhibited higher hydrophilicity than the latter The AP-GQD-MIP and AP-GQD-AuNP-MIP sensors were initially tested for detecting the cTnI epitope (ISASRKLQLK) in PBS buffer to evaluate the recognition capability of electro-MIPs for small peptide and their corresponding cavities.…”

Development of a Magnetic Molecularly Imprinted Microsphere-Based Signal Amplified Semi-Homogeneous Method for Multidetection of Five Progestins in Milk

“…In this study, PG was used as the template molecule to synthesize the magnetic molecularly imprinted microsphere (Fe 3 O 4 @MIM), which was carried out according to the principle of our previous report [31]. During the experiments, 0.1 mmol PG and 0.4 mmol function monomer MA were dissolved in 20 mL acetonitrile, and this solution was put into a tube to be pre-polymerized for 3 h at room temperature.…”