Surface pressure, surface potential and ellipsometric study of cytochrome c binding to dioleoylphosphatidylcholine monolayer at the air-water interface

Lamarche, F.; Téchy, F.; Aghion, Jacques; Leblanc, Roger M.

doi:10.1016/0166-6622(87)80210-x

Cited by 21 publications

(10 citation statements)

References 22 publications

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“…1, c and d), LTP exhibits comparable surface activity to that observed for the pure protein. Such a behavior has also been observed previously for cytochrome c (Lamarche et al, 1988), which is expelled from the lipid monolayer at its collapse pressure of 12 mN/m.…”

Section: Discussionsupporting

confidence: 83%

“…These results suggest that the physical nature of LTP films is the same whatever the spreading solvent. Similar results have been reported for cytochrome c spread at the air-water interface (Lamarche et al, 1988). The authors showed that the pro-cedure used to spread cytochrome c does not affect the physical properties of the resulting film and that the only parameter affected is the amount of protein remaining at the interface.…”

Section: Incorporation Of Ltp Into Lipid Monolayerssupporting

confidence: 81%

“…Solubilization of the protein into the subphase most likely occurs above this surface pressure. This value is of the same order of magnitude as that found for melittin (Fidelio et al, 1986) and is 9-10 mN/m higher than that obtained for other water-soluble proteins such as cytochrome c (Lamarche et al, 1988). This result suggests that LTP is a good surfaceactive agent and demonstrates its ability to spontaneously penetrate a lipid-free interface.…”

Section: Incorporation Of Ltp Into Lipid Monolayerssupporting

confidence: 57%

“…This suggests that part of the lipid has formed complexes with the protein that are expelled into the subphase above a surface pressure of 28 mN/in. If such complexes were not formed, all protein molecules should have been expelled in the subphase at the protein collapse pressure, and the rest of the isotherm should have been identical to that of the pure lipid as observed in the case of cytochrome c (Lamarche et al, 1988).…”

Section: Discussionmentioning

confidence: 99%

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Interaction of a nonspecific wheat lipid transfer protein with phospholipid monolayers imaged by fluorescence microscopy and studied by infrared spectroscopy

Subirade

Salesse

Marion

et al. 1995

Biophysical Journal

107

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The interaction of a nonspecific wheat lipid transfer protein (LTP) with phospholipids has been studied using the monolayer technique as a simplified model of biological membranes. The molecular organization of the LTP-phospholipid monolayer has been determined by using polarized attenuated total internal reflectance infrared spectroscopy, and detailed information on the microstructure of the mixed films has been investigated by using epifluorescence microscopy. The results show that the incorporation of wheat LTP within the lipid monolayers is surface-pressure dependent. When LTP is injected into the subphase under a dipalmytoylphosphatidylglycerol monolayer at low surface pressure (< 20 mN/m), insertion of the protein within the lipid monolayer leads to an expansion of dipalmytoylphosphatidylglycerol surface area. This incorporation leads to a decrease in the conformational order of the lipid acyl chains and results in an increase in the size of the solid lipid domains, suggesting that LTP penetrates both expanded and solid domains. By contrast, when the protein is injected under the lipid at high surface pressure (> or = 20 mN/m) the presence of LTP leads neither to an increase of molecular area nor to a change of the lipid order, even though some protein molecules are bound to the surface of the monolayer, which leads to an increase of the exposure of the lipid ester groups to the aqueous environment. On the other hand, the conformation of LTP, as well as the orientation of alpha-helices, is surface-pressure dependent. At low surface pressure, the alpha-helices inserted into the monolayers are rather parallel to the monolayer plane. In contrast, at high surface pressure, the alpha-helices bound to the surface of the monolayers are neither parallel nor perpendicular to the interface but in an oblique orientation.

show abstract

Section: Discussionsupporting

confidence: 83%

Section: Incorporation Of Ltp Into Lipid Monolayerssupporting

confidence: 81%

Section: Incorporation Of Ltp Into Lipid Monolayerssupporting

confidence: 57%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Interaction of a nonspecific wheat lipid transfer protein with phospholipid monolayers imaged by fluorescence microscopy and studied by infrared spectroscopy

Subirade

Salesse

Marion

et al. 1995

Biophysical Journal

107

View full text Add to dashboard Cite

show abstract

“…This behavior (of low reproducibility) is probably related to the formation of a bilayer. This hypothesis is based on the fact that DODAB readily forms vesicles in aqueous solution, which behave similarly to phospholipids, and on the indication that lipid bilayers exist at lateral pressures of 47-50 mN m −1 [33]. Fig.…”

Section: π -A Isothermsmentioning

confidence: 99%

Memory effects on the interfacial characteristics of dioctadecyldimethylammonium bromide monolayers at the air–water interface

Silva¹,

Romão²,

Lucero

et al. 2004

Journal of Colloid and Interface Science

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Photophysical Properties of Supramolecular Assemblies in Organized Films

Leblanc¹

1996

Physical Supramolecular Chemistry

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Surface pressure, surface potential and ellipsometric study of cytochrome c binding to dioleoylphosphatidylcholine monolayer at the air-water interface

Cited by 21 publications

References 22 publications

Interaction of a nonspecific wheat lipid transfer protein with phospholipid monolayers imaged by fluorescence microscopy and studied by infrared spectroscopy

Interaction of a nonspecific wheat lipid transfer protein with phospholipid monolayers imaged by fluorescence microscopy and studied by infrared spectroscopy

Memory effects on the interfacial characteristics of dioctadecyldimethylammonium bromide monolayers at the air–water interface

Photophysical Properties of Supramolecular Assemblies in Organized Films

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