Surface Properties of Surfactant-Free Oil Droplets Dispersed in Water Studied by Confocal Fluorescence Microscopy

Sakai, Toshio; Takeda, Yoshihiro; Mafuné, Fumitaka; Kondow, Tamotsu

doi:10.1021/jp036931p

Cited by 6 publications

(5 citation statements)

References 40 publications

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“…Here following selective encapsulation of single cells and mitochondria within droplets, we also demonstrate in droplets the rapid photolysis of single cells and the assay of enzymatic activity of a single cell. Although this paper focuses on aqueous droplets, we believe our method can be applied equally well to the formation and manipulation of organic droplets, which represent an active area of research. − …”

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Selective Encapsulation of Single Cells and Subcellular Organelles into Picoliter- and Femtoliter-Volume Droplets

et al. 2005

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This paper describes a method, which combines optical trapping and microfluidic-based droplet generation, for selectively and controllably encapsulating a single target cell or subcellular structure, such as a mitochondrion, into a picoliter- or femtoliter-volume aqueous droplet that is surrounded by an immiscible phase. Once the selected cell or organelle is encased within the droplet, it is stably confined in the droplet and cannot be removed. We demonstrate in droplet the rapid laser photolysis of the single cell, which essentially "freezes" the state that the cell was in at the moment of photolysis and confines the lysate within the small volume of the droplet. Using fluorescein di-beta-d-galactopyranoside, which is a fluorogenic substrate for the intracellular enzyme beta-galactosidase, we also assayed the activity of this enzyme from a single cell following the laser-induced lysis of the cell. This ability to entrap individual selected cells or subcellular organelles should open new possibilities for carrying out single-cell studies and single-organelle measurements.

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“…Although this paper focuses on aqueous droplets, we believe our method can be applied equally well to the formation and manipulation of organic droplets, which represent an active area of research. [23][24][25][26][27][28][29][30][31][32][33]…”

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Selective Encapsulation of Single Cells and Subcellular Organelles into Picoliter- and Femtoliter-Volume Droplets

et al. 2005

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“…Prior to that, a fluorescent dye known to interact with lipid surfaces, PKH67 was mixed with the emulsion assuming that the hydrophobic nature of oil would permit the dye insertion. [55] Indeed, as seen in Figure 5b numerous bright spots are recorded. Their size is in good agreement with that obtained from optical microscopy.…”

Section: Silicone Emulsification In Presence Of Hp-guar -Incorporatio...mentioning

confidence: 83%

Silicone incorporation into an esterquat based fabric softener in presence of guar polymers

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et al. 2021

Colloids and Surfaces A: Physicochemical and Engineering Aspect

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“…Crucially, the O/W interface acquires a negative charge even in the absence of emulsifiers such as surfactants 37) . Therefore, the oil droplets in EF-O/W emulsions typically have a negative surface potential (zeta potential) ranging from 30 to 60 mV 7,[9][10][11]17) . The TO droplets in the EF-TO/W emulsion also have a negative zeta potential, ranging from 30 to 60 mV (see Fig.…”

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Colloidal Stability of Emulsifier-free Triolein-in-Water Emulsions: Effects of Temperature

et al. 2022

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jjocs sustainable materials, and as a result, many manufacturers have focused on the development of EF-products 26−28) . Therefore, it is important to understand the fundamental nature of EF-emulsions: for example, the effect of the oil properties and the effects of the preparation and storage temperature on the colloidal stability of EF-O/W and EF-W/ O emulsions. Therefore, we have previously investigated the effects of the oil properties on the colloidal stability of EF-O/W and EF-W/O emulsions to evaluate the fundamentals of the colloidal stabilization of emulsions 4−16) .In contrast, in this work, the effect of the preparation and storage temperatures on the colloidal stability of EFtriolein-in-water (EF-TO/W) emulsions was examined because triolein (TO) is a typical triacylglycerol (TG) that is frequently used in foods, pharmaceuticals, and cosmetic products, and temperature is one of the most important parameters affecting the manufacture and storage of emul-Abstract: Herein, we report the colloidal stability of emulsifier-free (EF-) triolein-in-water (TO/W) emulsions prepared by mixing TO and water using a high-powered bath-type ultrasonicator (HPBath-US; 28 kHz, 300 W) in the absence of emulsifiers such as surfactants. In particular, the effect of the temperature (15-60℃) on the colloidal stability of EF-TO/W emulsions was examined because this is important for the practical application of EF-TO/W emulsions, for example, in foods, pharmaceuticals, and cosmetics. We found that the colloidal stability of the EF-TO/W emulsions decreased with increase in the temperature from 15 to 25℃, whereas it increased with increase in temperature from 25 to 40℃, and the high colloidal stability of the EF-TO/W emulsions was maintained above 40℃. The reduction in the colloidal stability of EF-TO/W emulsions between 15 and 25℃ is likely a result of the TO droplets formed by thermal motion, as well as enhanced Ostwald ripening at higher temperatures. On the other hand, the increase in the colloidal stability of the EF-TO/W emulsions from 25 to 40℃ and their high colloidal stability above 40℃ is attributed to the reduction in the interfacial tension between TO and water at higher temperatures. This decrease in the interfacial tension between TO and water with temperature increase is related to the transformation of short-range ordered domains (clusters) of TO molecules in the liquid state, which increases the colloidal stability of the EF-TO/W emulsions.

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Surface Properties of Surfactant-Free Oil Droplets Dispersed in Water Studied by Confocal Fluorescence Microscopy

Cited by 6 publications

References 40 publications

Selective Encapsulation of Single Cells and Subcellular Organelles into Picoliter- and Femtoliter-Volume Droplets

Selective Encapsulation of Single Cells and Subcellular Organelles into Picoliter- and Femtoliter-Volume Droplets

Silicone incorporation into an esterquat based fabric softener in presence of guar polymers

Colloidal Stability of Emulsifier-free Triolein-in-Water Emulsions: Effects of Temperature

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