Surface proteins of<i>Staphylococcus aureus</i>play an important role in experimental skin infection

Kwieciński, Jakub; Jin, Tao; Josefsson, Elisabet

doi:10.1111/apm.12295

Cited by 63 publications

(65 citation statements)

References 40 publications

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“…Also, mutations in genes of individual proteins responsible for clumping and/or coagulation, such as clfA , coa and vWbp , lead to decreased virulence. ClfA is involved in various types of infections including sepsis (Josefsson et al, 2008; McAdow et al, 2011), endocarditis (Moreillon et al, 1995; Que et al, 2001; Stutzmann Meier et al, 2001), kidney abscesses (Cheng et al, 2009), arthritis (Josefsson et al, 2001; Josefsson et al, 2008), and skin infections (Kwiecinski et al, 2014). There is, however, uncertainty regarding how ClfA affects virulence.…”

Section: Role Of Clumping/agglutination In Diseasementioning

confidence: 99%

“…Neutrophils are the predominant leukocytes in the blood and they are amongst the first phagocytes arriving at the infection site, while macrophages appear with a significant delay (Bremell et al, 1992; Kwiecinski et al, 2014). This sequence of events, combined with observed aggravated infection severity in mice lacking neutrophils (Molne et al, 2000; Verdrengh and Tarkowski, 1997), and in humans suffering from neutrophil-related disorders (Spaan et al, 2013), led some to speculate that neutrophils are the only cell type involved in the efficient response against staphylococci.…”

Section: Role Of Clumping/agglutination In Diseasementioning

confidence: 99%

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Staphylococcus aureus Aggregation and Coagulation Mechanisms, and Their Function in Host–Pathogen Interactions

Crosby

Kwieciński

Horswill

2016

Advances in Applied Microbiology

121

100

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The human commensal bacterium Staphylococcus aureus can cause a wide range of infections ranging from skin and soft tissue infections to invasive diseases like septicemia, endocarditis, and pneumonia. Muticellular organization almost certainly contributes to S. aureus pathogenesis mechanisms. While there has been considerable focus on biofilm formation and its role in colonizing prosthetic joints and indwelling devices, less attention has been paid to non-surface attached group behavior like aggregation and clumping. S. aureus is unique in its ability to coagulate blood, and it also produces multiple fibrinogen-binding proteins that facilitate clumping. Formation of clumps, which are large, tightly-packed groups of cells held together by fibrin(ogen), has been demonstrated to be important for S. aureus virulence and immune evasion. Clumps of cells are able to avoid detection by the host’s immune system due to a fibrin(ogen) coat that acts as a shield, and the size of the clumps facilitates evasion of phagocytosis. In addition, clumping could be an important early step in establishing infections that involve tight clusters of cells embedded in host matrix proteins, such as soft tissue abscesses and endocarditis. In this review we discuss clumping mechanisms and regulation, as well as what is known about how clumping contributes to immune evasion.

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Section: Role Of Clumping/agglutination In Diseasementioning

confidence: 99%

Section: Role Of Clumping/agglutination In Diseasementioning

confidence: 99%

Staphylococcus aureus Aggregation and Coagulation Mechanisms, and Their Function in Host–Pathogen Interactions

Crosby

Kwieciński

Horswill

2016

Advances in Applied Microbiology

121

100

View full text Add to dashboard Cite

show abstract

“…In the skin abscess model, a sortase-deficient Staphylococcus aureus strain lacking all of its cell-wall anchored proteins was less virulent than its wild-type strain. Also, strains specifically lacking protein A, fibronectin binding proteins, clumping factor A or surface protein SasF were impaired in their virulence (Josefsson et al, 2008; Kwiecinski et al, 2014). In addition some biofilm factors related to aggregation ability, for example, Bap protein of S. aureus facilitates the persistence in the mammary gland by enhancing adhesion to epithelial cells and prevents cellular internalization through the binding to GP96 host receptor (Taglialegna et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Shortening of the Lactobacillus paracasei subsp. paracasei BGNJ1-64 AggLb Protein Switches Its Activity from Auto-aggregation to Biofilm Formation

et al. 2016

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AggLb is the largest (318.6 kDa) aggregation-promoting protein of Lactobacillus paracasei subsp. paracasei BGNJ1-64 responsible for forming large cell aggregates, which causes auto-aggregation, collagen binding and pathogen exclusion in vitro. It contains an N-terminus leader peptide, followed by six successive collagen binding domains, 20 successive repeats (CnaB-like domains) and an LPXTG sorting signal at the C-terminus for cell wall anchoring. Experimental information about the roles of the domains of AggLb is currently unknown. To define the domain that confers cell aggregation and the key domains for interactions of specific affinity between AggLb and components of the extracellular matrix, we constructed a series of variants of the aggLb gene and expressed them in Lactococcus lactis subsp. lactis BGKP1-20 using a lactococcal promoter. All of the variants contained a leader peptide, an inter collagen binding-CnaB domain region (used to raise an anti-AggLb antibody), an anchor domain and a different number of collagen binding and CnaB-like domains. The role of the collagen binding repeats of the N-terminus in auto-aggregation and binding to collagen and fibronectin was confirmed. Deletion of the collagen binding repeats II, III, and IV resulted in a loss of the strong auto-aggregation, collagen and fibronectin binding abilities whereas the biofilm formation capability was increased. The strong auto-aggregation, collagen and fibronectin binding abilities of AggLb were negatively correlated to biofilm formation.

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“…In the present study, we used fluorescent intensity measurement and viable bacterial cell counting to represent the bacterial quantification in lesions, which can re- It has been reported that the survival of viable bacterial cells in lesions after the bacteria were injected for a certain period is considered as an important evidence for measuring bacterial virulence (18,26,27). In the present study, colonies of varying sizes were obtained after culturing the homogenized and diluted mice subcutaneous abscesses at 37°C ( Figure 5A).…”

Section: Fluorescence Reporter In S Aureus Contributes To the Study mentioning

confidence: 87%

Fluorescence Reporter in Staphylococcus aureus as a Useful Tool for Studying L-forms and Virulence

Wang

Sun

et al. 2017

Jundishapur J Microbiol

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Background: Staphylococcus aureus is a prominent pathogen responsible for human pyogenic and nosocomial infections. Although numerous studies on S. aureus Lforms and virulence are available in the literatures, there are still various impediments in the research methods employed. Objectives: This study aimed to perform fluorescence reporter in studying S. aureus L-form and its virulence, and wild strain virulence. Methods: Newman-pCM29 strain which can express green fluorescent protein (GFP) was constructed and used to detect S. aureus L-form formation and virulence in vitro and in vivo. Results: "Fried egg" like L-form colonies of GFP reporter S. aureus and the dense core of the colonies embedded into soft agar can be observed obviously. GFP reporter can contribute to preparing L-form suspension for injection and track the protoplasts and reverted cells in lesions of mice. GFP reporter, also, can help to detect S. aureus wild strain distribution and inflammation scope in lesions, and the fluorescence intensity can be used as an effective surrogate to represent the bacterial quantity in lesions. Fluorescence can improve to distinguish the colonies of varying sizes derived from the injected bacteria or from contaminants. Conclusions: Fluorescence reporter in S. aureus is a powerful tool which can provide new insight into S. aureus L-form formation in vitro and its fate in vivo, and wild strain virulence in vivo in a non-traditional manner.

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Surface proteins ofStaphylococcus aureusplay an important role in experimental skin infection

Cited by 63 publications

References 40 publications

Staphylococcus aureus Aggregation and Coagulation Mechanisms, and Their Function in Host–Pathogen Interactions

Staphylococcus aureus Aggregation and Coagulation Mechanisms, and Their Function in Host–Pathogen Interactions

Shortening of the Lactobacillus paracasei subsp. paracasei BGNJ1-64 AggLb Protein Switches Its Activity from Auto-aggregation to Biofilm Formation

Fluorescence Reporter in Staphylococcus aureus as a Useful Tool for Studying L-forms and Virulence

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