Surface quality and endothelial cell viability after femtosecond laser-assisted donor lenticule preparation for endothelial keratoplasty - An in-vitro study

Titiyal, Jeewan S; Aravind, M J; Kaur, Manpreet; Nag, Tapas Chandra; Sharma, Namrata; Agarwal, Tushar; Sinha, Rajesh

doi:10.4103/ijo.ijo_99_20

Cited by 1 publication

(1 citation statement)

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“…Previous studies have scrutinized the precision of FS-laser depth cuts with the Wavelight FS200 with minimal deviation between the achieved and attempted cut depths. 4,14 In addition, it is noteworthy that organ-cultured corneas are typically thicker than normal and more hydrated corneas. 15 Given that no dehydration process was conducted before the FS procedures, it is anticipated that the air-drying step for CAIRSs would result in significantly thinner segments.…”

Section: Discussionmentioning

confidence: 99%

Technique of corneal allogenic ring segment preparation using femtosecond laser: preclinical study on human corneal grafts

Mechleb,

Flamant,

Panthier

et al. 2024

J Cataract Refract Surg

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Purpose - This study aims to describe a novel technique for preparing Corneal Allogenic Ring Segments (CAIRS) using femtosecond laser (FSL) technology. Setting - Hospital Foundation Adolphe de Rothschild- Noémie de Rothschild institute. Design - Pre-clinical study conducted on human corneal grafts. Methods - The corneal grafts were mounted on an artificial chamber pressurized (ACP) with preset constant pressure, and the femtosecond laser was used to create a circular annulus with specific dimensions. The resulting CAIRS were analyzed for their thickness and width after air drying. Results: A total of 25 CAIRS were prepared using the femtosecond laser. The mean width and thickness of the CAIRS were 803 ± 77 μm and 83 ± 16 μm, respectively. Statistical analysis revealed no significant differences in width among the various quadrants of each CAIRS or between different CAIRS. Significantly thicker CAIRS were obtained with higher ACP pressure. Conclusion: The technique of CAIRS preparation using femtosecond laser technology and controlled artificial anterior chamber (AAC) pressure demonstrated reproducibility and precision. This approach holds the potential for customizing and personalizing CAIRS based on individual corneal characteristics.

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Section: Discussionmentioning

confidence: 99%