Surface reconstruction and tissue recognition for robotic-based in situ bioprinting

Fortunato, Gabriele Maria; Batoni, Elisa; Bonatti, Amedeo Franco; Vozzi, Giovanni; Maria, Carmelo De

doi:10.1016/j.bprint.2022.e00195

Cited by 18 publications

(19 citation statements)

References 28 publications

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“…The experimental part was carried out using IMAGObot, a robotic biofabrication platform developed in a previous study. − IMAGObot is a 5 DoF robotic arm designed starting from the open-source project MOVEO from BCN3D and re-engineered to be used for in situ bioprinting applications. IMAGObot is equipped with an electro-magnet as an end-effector allowing a fast and simple tool change to use different instruments during a single task . In this work, the photo-cross-linking device was integrated into the pneumatic-based EBB tool and used for bioprinting tests.…”

Section: Methodsmentioning

confidence: 99%

Automatic Photo-Cross-Linking System for Robotic-Based In Situ Bioprinting

Fortunato,

Batoni,

Pasqua

et al. 2023

ACS Biomater. Sci. Eng.

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This work reports the design and validation of an innovative automatic photo-cross-linking device for robotic-based in situ bioprinting. Photo-cross-linking is the most promising polymerization technique when considering biomaterial deposition directly inside a physiological environment, typical of the in situ bioprinting approach. The photo-cross-linking device was designed for the IMAGObot platform, a 5-degree-of-freedom robot re-engineered for in situ bioprinting applications. The system consists of a syringe pump extrusion module equipped with eight light-emitting diodes (LEDs) with a 405 nm wavelength. The hardware and software of the robot were purposely designed to manage the LEDs switching on and off during printing. To minimize the light exposure of the needle, thus avoiding its clogging, only the LEDs opposite the printing direction are switched on to irradiate the newly deposited filament. Moreover, the LED system can be adjusted in height to modulate substrate exposure. Different scaffolds were bioprinted using a GelMA-based hydrogel, varying the printing speed and light distance from the bed, and were characterized in terms of swelling and mechanical properties, proving the robustness of the photo-cross-linking system in various configurations. The system was finally validated onto anthropomorphic phantoms (i.e., a human humerus head and a human hand with defects) featuring complex nonplanar surfaces. The designed system was successfully used to fill these anatomical defects, thus resulting in a promising solution for in situ bioprinting applications.

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Section: Methodsmentioning

confidence: 99%

Automatic Photo-Cross-Linking System for Robotic-Based In Situ Bioprinting

Fortunato,

Batoni,

Pasqua

et al. 2023

ACS Biomater. Sci. Eng.

Self Cite

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show abstract

“…Additionally, the 3D printer having multiple degrees of freedom of various parts can help to minimize the side effects of invasive operation (Singh et al., 2020). A recent study reported surface reconstruction and mechanical evaluation of anatomical defects for robotic‐based in situ bioprinting applications (Fortunato et al., 2022). A touch probe as the end‐effector of a 5°‐of‐freedom https://www.sciencedirect.com/topics/engineering/robotic-arm was developed in this device.…”

Section: Scaffold‐based Methodsmentioning

confidence: 99%

Emerging tissue engineering strategies for the corneal regeneration

Tafti

Aghamollaei

Moghaddam

et al. 2022

J Tissue Eng Regen Med

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Cornea as the outermost layer of the eye is at risk of various genetic and environmental diseases that can damage the cornea and impair vision. Corneal transplantation is among the most applicable surgical procedures for repairing the defected tissue. However, the scarcity of healthy tissue donations as well as transplantation failure has remained as the biggest challenges in confront of corneal grafting. Therefore, alternative approaches based on stem-cell transplantation and classic regenerative medicine have been developed for corneal regeneration. In this review, the application and limitation of the recently-used advanced approaches for regeneration of cornea are discussed. Additionally, other emerging powerful techniques such as 5D printing as a new branch of scaffold-based technologies for construction of tissues other than the cornea are highlighted and suggested as alternatives for corneal reconstruction. The introduced novel techniques may have great potential for clinical applications in corneal repair including disease modeling, 3D pattern scheming, and personalized medicine.

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“…7 e) [ 179 ]. The spatially conformal path generated based on planar path projections can also fulfill a similar purpose [ 122 , 180 , 181 ], while it is only appropriate for the printing of single-layered scaffolds ( Fig. 7 f).…”

Section: Integration With Clinical Procedures For In Vivo ...mentioning

confidence: 99%

In vivo bioprinting: Broadening the therapeutic horizon for tissue injuries

Zhao

2023

Bioactive Materials

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Surface reconstruction and tissue recognition for robotic-based in situ bioprinting

Cited by 18 publications

References 28 publications

Automatic Photo-Cross-Linking System for Robotic-Based In Situ Bioprinting

Automatic Photo-Cross-Linking System for Robotic-Based In Situ Bioprinting

Emerging tissue engineering strategies for the corneal regeneration

In vivo bioprinting: Broadening the therapeutic horizon for tissue injuries

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