Surface specifically modified NK-92 cells with CD56 antibody conjugated superparamagnetic Fe<sub>3</sub>O<sub>4</sub> nanoparticles for magnetic targeting immunotherapy of solid tumors

Zhao, Sumei; Duan, Jiazhi; Lou, Yalin; Gao, Ruyun; Yang, Shanshan; Wang, Piming; Wang, Chunhua; Han, Lin; Li, Minghuan; Ma, Chao; Liang, Xiaohong; Liu, Hong; Sang, Yuanhua; Gao, Lifen

doi:10.1039/d1nr03329h

Cited by 19 publications

(9 citation statements)

References 37 publications

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“…抗原抗体之间的结合具有一定的强度，使得NK细胞可以利用这一作用力稳定地负载纳米配体。纳米配体可赋予NK细胞本身难以实现的功能，如磁靶向性。CD56是NK细胞重要的表面标志物。Zhao等［ 12 ］在2，3-二巯基丁二酸改性四氧化三铁纳米粒表面偶联抗CD56抗体，通过抗原抗体相互作用，将磁性纳米粒稳定地偶联在NK细胞表面，这一过程对NK细胞的增殖能力和细胞毒性没有影响，赋予NK细胞响应外界磁场迁移以及核磁共振示踪的能力。同时，抗原抗体之间的结合具有一定的可逆性，使得负载的纳米配体的释放变得灵活，参与NK细胞的协同治疗。例如，IL-21是NK细胞活化的关键细胞因子之一，通过在IL-21纳米凝胶中掺杂抗CD45抗体，可以稳定地负载在NK细胞表面。当肿瘤抗原刺激NK细胞时，该纳米凝胶中连接的二硫键会氧化还原响应性断裂而释放IL-21，从而实现NK细胞的持续激活［ 13 ］。…”

Section: Nk细胞表面修饰策略unclassified

Research progress in leveraging biomaterials for enhancing NK cell immunotherapy

TANG,

QIAN

2023

J Zhejiang Univ (Med Sci)

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NK细胞免疫疗法是继T细胞免疫疗法后另一种具有巨大临床前景的抗肿瘤方法。继基因工程之后，利用生物材料对NK细胞进行工程化改造也是精准、高效和低成本地构造完美人工免疫细胞的有效策略。首先，发展NK细胞表面修饰策略：通过脂质材料疏水效应、膜蛋白“受体-配体”之间非共价作用、膜蛋白的氨基酸残基共价结合、点击反应、静电相互作用等生物材料策略对NK细胞表面进行工程化修饰；其次，发展NK细胞内部修饰策略：通过纳米技术丰富NK细胞功能和NK细胞膜材料构建NK细胞仿生系统。另外，基于生物材料的技术策略被发展用于实体瘤内NK细胞原位激活：利用生物材料调控原位NK细胞在实体瘤部位的招募、识别和杀伤等功能，保障瘤内NK细胞活性，极大地改善NK细胞免疫疗法的治疗效果。本文重点综述基于生物材料增效NK细胞免疫疗法的相关研究进展。

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Section: Nk细胞表面修饰策略unclassified

Research progress in leveraging biomaterials for enhancing NK cell immunotherapy

TANG,

QIAN

2023

J Zhejiang Univ (Med Sci)

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“…For instance, Zhao et al have developed a functionalized Fe 3 O 4 NPs modified with the CD56 antibodies, which can specifically bind with NK‐92 cells surface. These NK‐Fe 3 O 4 biohybrid NPs were able to promote the recruitment and infiltration of exogenous NK‐92 cells into solid tumors under external magnetic field guidance and significantly inhibit tumor growth (Zhao et al, 2021). Another similar study is nanoengineered immunotherapy of NK cells conjugating iron oxide nanoparticles IONPs onto the cell surface, which provides an additional property of magnetic homing to the NK cells, improving their therapeutic function.…”

Section: Surface Nanoengineering Strategies Based Live Cellsmentioning

confidence: 99%

Cell surface‐nanoengineering for cancer targeting immunoregulation and precise immunotherapy

Wang

Huang

Hou

et al. 2022

WIREs Nanomed Nanobiotechnol

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Living cells have become ideal therapeutic agents for cancer treatment owing to their innate activities, such as efficient tumor targeting and delivery, easy engineering, immunomodulatory properties, and fewer adverse effects. However, cell agents are often fragile to rigorous tumor microenvironment (TME) and limited by inadequate therapeutic responses, leading to unwanted treatment efficacy. Cell nanomodification, particularly the cell surfacenanoengineering has emerged as reliable and efficient strategy that not only combines cell activity properties with nanomaterials but also endows them with extra novel functions, enabling to achieve remarkable treatment results. In this review, we systematically introduce two major strategies have been adopted to develop cell surface engineering with nanomaterials, mainly including living cell nano-backpacks and cell membrane-mimicking nanoparticles (NPs). Based on various functional NPs and cell types, we focus on reviewing the cell-surface nanoengineering for targeted drug delivery, immune microenvironment regulation, and precisely antitumor therapy. The advances and challenges of cell surface-nanoengineered antitumor agents for cancer therapy applications are further discussed in future clinical practice. This review provides an overview of the advances in cell surface-engineering for targeting immunoregulation and treatment and could contribute to the future of advanced cell-based antitumor therapeutic applications.

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“…Finally, by synergizing immune cells with a directional magnetic field that promotes infiltration into solid tumor tissue under magnetic resonance imaging control, antitumor efficiency is significantly improved. Magnetic nanoparticles and NK cells can be utilized for various biomedical applications, as they have proved to possess flexible characteristics to operate in biomedicine[ 58 ].…”

Section: Types Of Cancer Nanomedicinesmentioning

confidence: 99%

Nanomedicine approaches for treatment of hematologic and oncologic malignancies

Nteli¹,

Bajwa²,

Politakis³

et al. 2022

WJCO

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Cancer is a leading cause of death worldwide. Nowadays, the therapies are inadequate and spur demand for improved technologies. Rapid growth in nanotechnology and novel nanomedicine products represents an opportunity to achieve sophisticated targeting strategies and multi-functionality. Nanomedicine is increasingly used to develop new cancer diagnosis and treatment methods since this technology can modulate the biodistribution and the target site accumulation of chemotherapeutic drugs, thereby reducing their toxicity. Cancer nanotechnology and cancer immunotherapy are two parallel themes that have emerged over the last few decades while searching for a cure for cancer. Immunotherapy is revolutionizing cancer treatment, as it can achieve unprecedented responses in advanced-stage patients, including complete cures and long-term survival. A deeper understanding of the human immune system allows the establishment of combination regimens in which immunotherapy is combined with other treatment modalities (as in the case of the nanodrug Ferumoxytol). Furthermore, the combination of gene therapy approaches with nanotechnology that aims to silence or express cancer-relevant genes via one-time treatment is gradually progressing from bench to bedside. The most common example includes lipid-based nanoparticles that target VEGF-Α and KRAS pathways. This review focuses on nanoparticle-based platforms utilized in recent advances aiming to increase the efficacy of currently available cancer therapies. The insights provided and the evidence obtained in this paper indicate a bright future ahead for immuno-oncology applications of engineering nanomedicines.

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Surface specifically modified NK-92 cells with CD56 antibody conjugated superparamagnetic Fe₃O₄ nanoparticles for magnetic targeting immunotherapy of solid tumors

Abstract: Although there has been significant progress in the development of tumor immunotherapies, many challenges still exist for the treatment of solid tumors. Natural killer (NK) cells possess broad-spectrum cytotoxicity against...

Cited by 19 publications

References 37 publications

Research progress in leveraging biomaterials for enhancing NK cell immunotherapy

Research progress in leveraging biomaterials for enhancing NK cell immunotherapy

Cell surface‐nanoengineering for cancer targeting immunoregulation and precise immunotherapy

Nanomedicine approaches for treatment of hematologic and oncologic malignancies

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Surface specifically modified NK-92 cells with CD56 antibody conjugated superparamagnetic Fe3O4 nanoparticles for magnetic targeting immunotherapy of solid tumors

Abstract: Although there has been significant progress in the development of tumor immunotherapies, many challenges still exist for the treatment of solid tumors. Natural killer (NK) cells possess broad-spectrum cytotoxicity against...

Cited by 19 publications

References 37 publications

Research progress in leveraging biomaterials for enhancing NK cell immunotherapy

Research progress in leveraging biomaterials for enhancing NK cell immunotherapy

Cell surface‐nanoengineering for cancer targeting immunoregulation and precise immunotherapy

Nanomedicine approaches for treatment of hematologic and oncologic malignancies

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Product

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Surface specifically modified NK-92 cells with CD56 antibody conjugated superparamagnetic Fe₃O₄ nanoparticles for magnetic targeting immunotherapy of solid tumors