Surface stabilization treatment enhances initial cell viability and adhesion for biodegradable zinc alloys

Levy, Galit Katarivas; Kafri, Alon; Ventura, Yvonne; Leon, Avi; Vago, Razi; Goldman, Jeremy; Aghion, Eli

doi:10.1016/j.matlet.2019.04.006

Cited by 36 publications

(22 citation statements)

References 10 publications

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“…Regarding the pre-treated Zn sample, EDS analysis indicated that the main elemental components were Zn, C, O and Cl. The results indicate the formation of oxide and carbonate degradation products, which is consistent with previous studies [ 27 , 28 , 29 ].…”

Section: Resultssupporting

confidence: 92%

“…Pure Zn was further pre-treated with a complete cell culture medium under standard cell culture conditions (denoted as P-Zn). The formation of the initial degradation layer can partially mimic the degradation interface changes occurring in the early post-implantation stage, as previously reported [27][28][29]. Specifically, Zn discs were immersed in 2 mL Dulbecco's modified Eagle medium (DMEM, Gibco, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (FBS, ExCell Bio, Shanghai, China) under standard cell culture conditions for 7 days.…”

Section: Specimen Preparation and Surface Characterizationmentioning

confidence: 99%

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Limitation of Water-Soluble Tetrazolium Salt for the Cytocompatibility Evaluation of Zinc-Based Metals

Zhu

2021

Materials

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Zinc (Zn) and its alloys have been regarded as promising biodegradable metals. The standardized cytotoxicity evaluation is a mandatory step to screen the biocompatibility of novel Zn and its alloys. Nevertheless, the suitability of the tetrazolium-based assay in the direct contact test for some metallic biomaterials (i.e., magnesium and manganese) is questionable. In this study, our results demonstrate an obvious inconsistency between qualitative observation via fluorescence staining and quantitative assessment using water-soluble tetrazolium salt (CCK-8). Subsequent experiments revealed that Zn and pre-treated Zn can directly convert tetrazolium salts to formazan, falsifying the cytotoxicity results. Therefore, we conclude that the CCK-8 assay is not suitable for evaluating the cytotoxicity of biodegradable Zn-based metals in the direct contact test.

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Section: Resultssupporting

confidence: 92%

Section: Specimen Preparation and Surface Characterizationmentioning

confidence: 99%

Limitation of Water-Soluble Tetrazolium Salt for the Cytocompatibility Evaluation of Zinc-Based Metals

Zhu

2021

Materials

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“…Human endothelial cell (EA.hy926) Improved cell proliferation Biomimetic deposition Zn-1.5Mg Immersing in SBF for two weeks Calcium phosphate MEM cultivation medium with 5% fetal bovine serum 0.019 ± 0.011 (mg cm −2 day −1 , with CO 2 atmosphere); 0.004 ± 0.002 (mg cm −2 day −1 , without CO 2 atmosphere); i. Murine fibroblast (L929) Improved cell viability [ 155 ] ii. Human osteosarcoma cell (U-2 OS) Improved cell adhesion number with more spreading Zn–3Cu–1Mg Immersing in Ca(H 2 PO 4 ) ·H 2 O, sodium nitrate and hydrogen peroxide mixture solution for 1d at 20 °C CaHPO 4 ·2H 2 O Bone marrow mesenchymal stem cell (BMSC) Improved cell proliferation, osteogenic differentiation and calcium deposition [ 191 ] Stabilization treatment Zn–1Mg Immersing in Dulbecco's Modified Eagle's Medium for 1 day under a humidified atmosphere with 5% CO 2 at 37 °C ZnO and Zn(OH) 2 Murine mesenchymal stem cell (MSC) Improved cell adhesion and proliferation [ 192 ] Zn–1Mg-0.5Ca Improved cell adhesion and proliferation Electrochemical method Microarc oxidation Pure Zn (99.999%) 400 V, 3 min, electrolyte: 2.5 g/L sodium hydroxide and 0.02 mol/L calcium glycerophosphate hydrate …”

Section: Surface Modification Of Zn-based Bms For Biomedical Applicationsmentioning

confidence: 99%

A review on current research status of the surface modification of Zn-based biodegradable metals

Yuan

Xia

et al. 2022

Bioactive Materials

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Recently, zinc and its alloys have been proposed as promising candidates for biodegradable metals (BMs), owning to their preferable corrosion behavior and acceptable biocompatibility in cardiovascular, bone and gastrointestinal environments, together with Mg-based and Fe-based BMs. However, there is the desire for surface treatment for Zn-based BMs to better control their biodegradation behavior. Firstly, the implantation of some Zn-based BMs in cardiovascular environment exhibited intimal activation with mild inflammation. Secondly, for orthopedic applications, the biodegradation rates of Zn-based BMs are relatively slow, resulting in a long-term retention after fulfilling their mission. Meanwhile, excessive Zn 2+ release during degradation will cause in vitro cytotoxicity and in vivo delayed osseointegration. In this review, we firstly summarized the current surface modification methods of Zn-based alloys for the industrial applications. Then we comprehensively summarized the recent progress of biomedical bulk Zn-based BMs as well as the corresponding surface modification strategies. Last but not least, the future perspectives towards the design of surface bio-functionalized coatings on Zn-based BMs for orthopedic and cardiovascular applications were also briefly proposed.

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“…Due to their superior mechanical properties, excellent corrosion resistance, and outstanding biocompatibility, Ti6Al4V alloys have been used as biomedical implants and aviation materials [9][10][11][12]. However, the inherent difficulties in plastic processing and specific components with complex geometry make Ti6Al4V an attractive material for AM.…”

Section: Introductionmentioning

confidence: 99%

Nano-Mechanical Properties and Creep Behavior of Ti6Al4V Fabricated by Powder Bed Fusion Electron Beam Additive Manufacturing

et al. 2021

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Effects of scanning strategy during powder bed fusion electron beam additive manufacturing (PBF-EB AM) on microstructure, nano-mechanical properties, and creep behavior of Ti6Al4V alloys were compared. Results show that PBF-EB AM Ti6Al4V alloy with linear scanning without rotation strategy was composed of 96.9% α-Ti and 2.7% β-Ti, and has a nanoindentation range of 4.11–6.31 GPa with the strain rate ranging from 0.001 to 1 s−1, and possesses a strain-rate sensitivity exponent of 0.053 ± 0.014. While PBF-EB AM Ti6Al4V alloy with linear and 90° rotate scanning strategy was composed of 98.1% α-Ti and 1.9% β-Ti and has a nanoindentation range of 3.98–5.52 GPa with the strain rate ranging from 0.001 to 1 s−1, and possesses a strain-rate sensitivity exponent of 0.047 ± 0.009. The nanohardness increased with increasing strain rate, and creep displacement increased with the increasing maximum holding loads. The creep behavior was mainly dominated by dislocation motion during deformation induced by the indenter. The PBF-EB AM Ti6Al4V alloy with only the linear scanning strategy has a higher nanohardness and better creep resistance properties than the alloy with linear scanning and 90° rotation strategy. These results could contribute to understanding the creep behavior of Ti6Al4V alloy and are significant for PBF-EB AM of Ti6Al4V and other alloys.

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Surface stabilization treatment enhances initial cell viability and adhesion for biodegradable zinc alloys

Cited by 36 publications

References 10 publications

Limitation of Water-Soluble Tetrazolium Salt for the Cytocompatibility Evaluation of Zinc-Based Metals

Limitation of Water-Soluble Tetrazolium Salt for the Cytocompatibility Evaluation of Zinc-Based Metals

A review on current research status of the surface modification of Zn-based biodegradable metals

Nano-Mechanical Properties and Creep Behavior of Ti6Al4V Fabricated by Powder Bed Fusion Electron Beam Additive Manufacturing

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