Surface Topography Hinders Bacterial Surface Motility

Chang, Yow-Ren; Weeks, Eric R.; Ducker, William A.

doi:10.1021/acsami.7b16715

Cited by 53 publications

(68 citation statements)

References 55 publications

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“…Their work showed that the imprinted polymer nanostructures with precisely defined geometries can prevent bacteria biofilm formation . More recently, Ducker's group demonstrated that “the nanometer barrier” significantly reduce the surface motility of the bacterium and may therefore hinder surface biofilm expansion . Despite a promising technique, the long‐term antifouling efficacy in the surface topographically modified catheter model has not been reported.…”

Section: Antimicrobial Strategies For Urinary Cathetersmentioning

confidence: 99%

“…162 More recently, Ducker's group demonstrated that "the nanometer barrier" significantly reduce the surface motility of the bacterium and may therefore hinder surface biofilm expansion. 163,165 Despite a promising technique, the long-term antifouling efficacy in the surface topographically modified catheter model has not been reported. CONCLUSION CAUTI is one of the most prevalent hospital-acquired infections, attributed to an extensive application of catheters in healthcare facilities and hospital stay.…”

Section: Bacterial Interferencementioning

confidence: 99%

See 1 more Smart Citation

Antimicrobial strategies for urinary catheters

Zhu

Wang

et al. 2018

J Biomedical Materials Res

110

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Over 75% of hospital‐acquired or nosocomial urinary tract infections are initiated by urinary catheters, which are used during the treatment of 16% of hospitalized patients. Taking the United States as an example, the costs of catheter‐associated urinary tract infections (CAUTI) are in excess of $451 million dollars/year. The biofilm formation by pathogenic microbes that protects pathogens from host immune defense and antimicrobial agents is the leading cause for CAUTI. Thus, tremendous efforts have been devoted to antimicrobial coating for urinary catheters in the past few decades, and it has been demonstrated to be one of the most direct and efficient strategies to reduce infections. In this article, we briefly summarize the current methods for preparation of antimicrobial coatings based on different stages in the biofilm formation, highlight recent progress in the urinary catheter coating material design and selection, discuss approaches to improving their long‐term antimicrobial efficacy, biocompatibility, multidrug resistance and recurrent infections, and finally outline future requirements and prospects in antimicrobial coating material design. The scope of the works surveyed is confined to antimicrobial urinary catheters. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 445–467, 2019.

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Section: Antimicrobial Strategies For Urinary Cathetersmentioning

confidence: 99%

Section: Bacterial Interferencementioning

confidence: 99%

Antimicrobial strategies for urinary catheters

Zhu

Wang

et al. 2018

J Biomedical Materials Res

110

View full text Add to dashboard Cite

show abstract

“…It has been suggested that microorganisms tend to maximize the contact area with a surface 15 , or preferentially bind to locations that maximize the number of attachment points 16 . Recently, these mechanisms of selectivity have been associated with a cost of cell adhesion, since surface arrangements containing local curvature at the micron and submicron scale (e.g., hemispheres patterns) seem to be unfavorable for bacterial attachment despite their large surface area 11,17 . Moreover, using recessed patterns inspired by the Shark’s skin, Sakamoto and coworkers 18 showed that the geometric complexity of the surface (e.g., tortuosity) rather than the feature depth in grooved patterns, could reduce biofilm formation and the swarming motility in both Pseudomonas aeruginosa and Staphylococcus aureus strains.…”

Section: Introductionmentioning

confidence: 99%

E. coliadhesion and biofilm formation on polydimethylsiloxane are independent of substrate stiffness

Arias

Devorkin

Civantos

et al. 2020

Preprint

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AbstractBacterial adhesion and biofilm formation on the surface of biomedical devices is a detrimental process that compromises patient safety and material functionality. Several physicochemical factors are involved in biofilm growth, including the surface properties. Among those, material stiffness has recently been suggested to influence microbial adhesion and biofilm growth in a variety of polymers and hydrogels. However, no clear consensus exists about the role of material stiffness on biofilm initiation and whether very compliant substrates are deleterious to bacterial cell adhesion. Here, by systematically tuning substrate topography and stiffness while keeping the surface free energy of polydimethylsiloxane substrates constant, we show that topographical patterns at the micron and submicron scale impart unique properties to the surface that are independent of the material stiffness. The current work provides a better understanding of the role of material stiffness on bacterial physiology and may constitute a cost-effective and simple strategy to reduce bacterial attachment and biofilm growth even in very compliant and hydrophobic polymers.

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“…For instance, the pili attachment is enhanced 2,18,21 when the substratum is covered by extracellular polymeric materials. Patterned surfaces can be a barrier for bacterial twitching and hence hinder surface exploration by bacteria 7,22 . Chang, et al 22 have shown that micro-scale surface topography (pillars) appears to be a barrier to the surface motility of Pseudomonas aeruginosa and it may hinder the ability of such cells to explore a surface.…”

Section: Introductionmentioning

confidence: 99%

“…Patterned surfaces can be a barrier for bacterial twitching and hence hinder surface exploration by bacteria 7,22 . Chang, et al 22 have shown that micro-scale surface topography (pillars) appears to be a barrier to the surface motility of Pseudomonas aeruginosa and it may hinder the ability of such cells to explore a surface. However, when the surface has micro-scale grooves, bacteria may display persistent twitching along grooves because cells can be guided by the groove walls 2,23 .…”

Section: Introductionmentioning

confidence: 99%

Modelling bacterial twitching in fluid flows: a CFD-DEM approach

Jayathilake

Zuliani

et al. 2019

Preprint

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Bacterial habitats are often associated with fluid flow environments. There is a lack of models 10 of the twitching motility of bacteria in shear flows. In this work, a three-dimensional modelling 11 approach of Computational Fluid Dynamics (CFD) coupled with the Discrete Element Method 12 (DEM) is proposed to study bacterial twitching on flat and groove surfaces under shear flow 13 conditions. Rod-shaped bacteria are modelled as groups of spherical particles and Type IV pili 14 attached to bacteria are modelled as dynamic springs which can elongate, retract, attach and 15 detach. The CFD-DEM model of rod-shape bacteria is validated against orbiting of immotile 16 bacteria in shear flows. The effects of fluid flow rate and surface topography on twitching 17 motility are studied. The model can successfully predict upstream twitching motility of rod-18 shaped bacteria in shear flows. Our model can predict that there would be an optimal range of 19 wall shear stress in which bacterial upstream twitching is most efficient. The results also 20 indicate that when bacteria twitch on groove surfaces, they are likely to accumulate around the 21 downstream side of the groove walls.22 23 24 25 29the surface using appendages called type IV pili (TFP) 2-5 to "explore" the substratum to find 30 suitable sites for growth and thus biofilm formation. Pili emanate from bacterial surface and 31 † The author is currently affiliated with the Department of Oncology, University of Oxford, UK.2 they can be up to several µm long (though they are nm in diameter 6 ). Bacterial twitching 32 occurs through cycles of polymerization and de-polymerization of type IV pili 7,8 . 33Polymerization causes the pilus to elongate and eventually attaching into surfaces. De-34 polymerization makes the pilus to retract and detaching from the surfaces. Pili retraction 35 produces pulling forces on the bacterium, which will be pulled in the direction of the vector 36 sum of the pili forces, resulting in a jerky movement (Figure 1). A typical TFP can produce a 37 force exceeding 100 pN 9 and then a bundle of pili can produce pulling forces up to several nN 38 10 . Bacteria may use pili not only for twitching but also for cell-cell interactions 11,12 , surface 39 sensing 13,14 and DNA uptake 15 . 40Twitching motility could depend on many factors including surface properties, pili 41 arrangement on bacterial surface, and environmental conditions such as oxygen concentration 42 and fluid flow rate 16 . For example, when pili emanate only at the poles of bacteria (e.g., 43Pseudomonas aeruginosa), the bacteria will have persistent motion 17,18 . But, if pili are all 44 around the cell body (e.g., Neisseria gonorrhoeae), the bacteria will have trapped or diffusive 45 motion due to the tug of war mechanism 19,20 . If a pilus detaches while all the pili are in high 46 tension and anti-parallel configuration, the bacterium will suddenly align along the resultant 47 direction of the remaining bounded-pili causing a sudden change of the twitching direction. 48This is ...

show abstract

Surface Topography Hinders Bacterial Surface Motility

Cited by 53 publications

References 55 publications

Antimicrobial strategies for urinary catheters

Antimicrobial strategies for urinary catheters

E. coliadhesion and biofilm formation on polydimethylsiloxane are independent of substrate stiffness

Modelling bacterial twitching in fluid flows: a CFD-DEM approach

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