Surface Water Microbial Community Response to the Biocide 2,2-Dibromo-3-Nitrilopropionamide, Used in Unconventional Oil and Gas Extraction

Campa, Maria Fernanda; Techtmann, Stephen M.; Ladd, Mallory P.; Yan, Jun; Patterson, Megan; Amaral, Amanda Garcia de Matos; Carter, Kimberly E.; Ulrich, Nikea; Grant, Christopher J.; Hettich, Robert L.; Lamendella, Regina; Hazen, Terry C.

doi:10.1128/aem.01336-19

Cited by 19 publications

(18 citation statements)

References 73 publications

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“…The bacterial communities had different responses to the presence of DBNPA. DBNPA remained longer in the communities that had been exposed to the biocides previously, but these microbial communites were more tolerant to the biocide (16). A similar study was done with glutaraldehyde was done and they found similar results.…”

Section: Impact On Aquatic Communitiessupporting

confidence: 52%

Biocide Resistance as a Result of Exposure to Biocides Used in Hydraulic Fracturing

Schenten¹

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Section: Impact On Aquatic Communitiessupporting

confidence: 52%

Biocide Resistance as a Result of Exposure to Biocides Used in Hydraulic Fracturing

Schenten¹

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“…We have recently showed that streams impacted by UOG spills containing glutaraldehyde are more resistant to the biocide when exposed to it again as compared to pristine streams (Campa et al, 2018). However, the resistance was biocide-specific, as DBNPA did not show enhanced resistance in the same streams compared to pristine streams (Campa et al, 2019). More studies providing clear evidence of environmental resistance and biocide-biocide and biocide-antibiotic cross resistance are needed to determine which biocides minimize health and environmental risk.…”

Section: Antimicrobial Resistance Enrichment Potential In the Environmentioning

confidence: 99%

Unconventional Oil and Gas Energy Systems: An Unidentified Hotspot of Antimicrobial Resistance?

et al. 2019

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Biocides used in unconventional oil and gas (UOG) practices, such as hydraulic fracturing, control microbial growth. Unwanted microbial growth can cause gas souring, pipeline clogging, and microbial-induced corrosion of equipment and transportation pipes. However, optimizing biocide use has not been a priority. Moreover, biocide efficacy has been questioned because microbial surveys show an active microbial community in hydraulic fracturing produced and flowback water. Hydraulic fracturing produced and flowback water increases risks to surface aquifers and rivers/lakes near the UOG operations compared with conventional oil and gas operations. While some biocides and biocide degradation products have been highlighted as chemicals of concern because of their toxicity to humans and the environment, the selective antimicrobial pressure they cause has not been considered seriously. This perspective article aims to promote research to determine if antimicrobial pressure in these systems is cause for concern. UOG practices could potentially create antimicrobial resistance hotspots under-appreciated in the literature, practice, and regulation arena, hotspots that should not be ignored. The article is distinctive in discussing antimicrobial resistance risks associated with UOG biocides from a biological risk, not a chemical toxicology, perspective. We outline potential risks and highlight important knowledge gaps that need to be addressed to properly incorporate antimicrobial resistance emergence and selection into UOG environmental and health risk assessments.

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“…Degradation of GA by the microbial community was also observed during the process. Campa and colleagues also investigated the microbial community response to 2,2-dibromo-3-nitrilopropionamide (DBNPA) following a similar workflow ( 14 ). In that study, the microbial response to DBNPA was distinct, compared with the response to GA, and a different microbial community structure resulted from the DBNPA treatment ( 14 ).…”

Section: Introductionmentioning

confidence: 99%

“…Campa and colleagues also investigated the microbial community response to 2,2-dibromo-3-nitrilopropionamide (DBNPA) following a similar workflow ( 14 ). In that study, the microbial response to DBNPA was distinct, compared with the response to GA, and a different microbial community structure resulted from the DBNPA treatment ( 14 ). To our knowledge, however, no studies have yet explored the relationships between biocide performance and the ecological properties of a souring microbial community during biocide treatment and after biocide failure, particularly how the ecological property changes could reflect the failure of biocides in souring control.…”

Section: Introductionmentioning

confidence: 99%

Long-Term Biocide Efficacy and Its Effect on a Souring Microbial Community

Shi

Oliveira

Holsten³

et al. 2021

Appl Environ Microbiol

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Reservoir souring, which is the production of H 2 S mainly by sulfate-reducing microorganisms (SRM) in oil reservoirs, has been a long-standing issue for the oil industry. While biocides have been frequently applied to control biogenic souring, the effects of biocide treatment are usually temporary, and biocides eventually fail. The reasons behind biocide failure and the long-term response of the microbial community remain poorly understood. In this study, one time biocide treatments with glutaraldehyde (GA) and an aldehyde-releasing biocide (ARB) at low (100 ppm) and high (750 ppm) dosages were individually applied to a complex sulfate-reducing microbial community, followed by one-year monitoring of the chemical responses and the microbial community succession. The chemical results showed that souring control failed after 7 days at 100 ppm dosage regardless the biocide type, and that lasting souring control for the entire one-year timespan was only achieved with ARB at 750 ppm. Microbial community analyses suggested that the high dosage biocide treatments resulted in one order of magnitude lower average total microbial abundance and average SRM abundance compared to the low dosage treatments. The recurrence of souring was associated with reduction of alpha diversity and with long-term microbial community structure change, thus monitoring changes in microbial community metrics may serve as early warnings of the failure of a biocide-based souring control programme in the field. Furthermore, spore-forming sulfate reducers ( Desulfotomaculum and Desulfurispora ) were enriched and became dominant in both GA-treated groups, which could cause challenges to the design of long-lasting remedial souring control strategies. IMPORTANCE Reservoir souring is a detrimental problem for the oil and gas industry as H 2 S corrodes the steel infrastructure, downgrades the oil quality and poses substantial risks to the field personnel and the environment. Biocides have been widely applied to remedy souring, yet the long-term performance of biocide treatments is hard to predict or optimise due to limited understanding of the microbial ecology affected by biocide treatment. This study investigates the long-term biocide performance and associated changes in the abundance, diversity and structure of the souring microbial community, thus advancing the knowledge towards a deeper understanding of the microbial ecology of biocide-treated systems, and contributing to the improvement of current biocide-based souring control practices. The study showcases the potential application of incorporating microbial community analyses to forecast souring and highlights the long-term consequences of the biocide treatment on the microbial communities, with relevance to both operators and regulators.

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Surface Water Microbial Community Response to the Biocide 2,2-Dibromo-3-Nitrilopropionamide, Used in Unconventional Oil and Gas Extraction

Cited by 19 publications

References 73 publications

Biocide Resistance as a Result of Exposure to Biocides Used in Hydraulic Fracturing

Biocide Resistance as a Result of Exposure to Biocides Used in Hydraulic Fracturing

Unconventional Oil and Gas Energy Systems: An Unidentified Hotspot of Antimicrobial Resistance?

Long-Term Biocide Efficacy and Its Effect on a Souring Microbial Community

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