Surfactant-assisted alkaline pretreatment and enzymatic hydrolysis of Miscanthus sinensis for enhancing sugar recovery with a reduced enzyme loading

Abstract

Surfactants play a vital role in the delignification and saccharification of lignocellulosic biomass. A strategy for coupling surfactant-assisted alkaline pretreatment (SAP) with surfactant-assisted enzymatic hydrolysis (SEH) has been proposed for improving sugar recovery from a potential energy crop, Miscanthus sinensis. Poly (ethylene glycol) 2000 (PEG 2000) was found to be more efficient in SAP than in other tested surfactants. Compositional and structural analysis revealed that the SAP process with 1% of PEG 2000 produced more efficient lignin removal and microstructure disruption of the pretreated sample, thus indicating much higher reducing sugar yields of 544.4–601.2 mg/g compared to the samples that were untreated or pretreated by alkali alone. Moreover, SEH with 1% Tween 80, which could block the lignin-enzyme interactions, produced a substantial reduction of 33.3% in the enzyme loading to achieve a higher sugar recovery from the SAP sample.

Robust pretreatment in combination with ultrasound-assisted surfactant to improve the enzymatic hydrolysis of Eucalyptus pellita

“…Additionally, it is plausible that the treatment dissolves hemicelluloses associated with or covering lignin, thereby augmenting cellulase accessibility and subsequent cellulose adsorption. Extending the treatment duration may further enhance accessibility, consequently increasing the expected glucose yield (Ghavi et al 2021;Cheng et al 2022). However, further research is imperative to establish a definitive correlation between surfactant effects and substrate characteristics.…”

“…Surfactants play a vital role in the pretreatment and enzymatic hydrolysis of lignocellulosic biomass. They can help to enhance the delignification and saccharification of the biomass, thereby improving the yield of reducing sugars (Cheng et al 2022). According to the experiment done by Cheng et al a strategy for coupling surfactantassisted alkaline pretreatment with surfactant-assisted enzymatic hydrolysis has been proposed for improving sugar recovery from lignocellulosic biomass and results showed that the surfactant-assisted alkaline pretreatment process with 1% of PEG 2000 resulted in more efficient lignin removal and microstructure disruption of the pretreated sample, thus indicating much higher reducing sugar yields.…”

“…According to the experiment done by Cheng et al a strategy for coupling surfactantassisted alkaline pretreatment with surfactant-assisted enzymatic hydrolysis has been proposed for improving sugar recovery from lignocellulosic biomass and results showed that the surfactant-assisted alkaline pretreatment process with 1% of PEG 2000 resulted in more efficient lignin removal and microstructure disruption of the pretreated sample, thus indicating much higher reducing sugar yields. (Cheng et al 2022).…”

“…Comparing to the experiment done by Elalami et al (2022), the highest glucose yield was 86% obtained by ultrasonication coupled to alkaline pretreatment of bean straw. Cheng et al (2022) demonstrated that surfactant-assisted alkaline pretreatment (SAP) with elevated surfactant concentrations (1% vs. 0.5%) increased sugar yields. SAP assisted by 1% surfactant concentration showed improved results.…”

Surfactant-assisted hydrothermal pretreatment for co- production of reducing sugars and carbon quantum dots from lignocellulosic biomass

“…The quantum yield (QY) was measured by Fluorescence (F-7100) and UV-visible spectrophotometer (UV-2700), and the data of QY was calculated according to Eq. ( 4) (Li X X et al, 2022):…”