Surfactant-Induced Alteration of Arachidonic Acid Metabolism of Mammalian Cells in Culture

Abstract

Primary irritancy in human and animal skin is characterized by an inflammatory reaction mediated, in part, by membranederived arachidonate metabolites. One of the mechanisms of this reaction was investigated in cultured mammalian cells using three surfactants: linear alkyl benzene sulfonate (LAS), alkyl ethoxylate sulfate (AEOS), and TWEEN 20. These compounds 477

Assessment of potency of allergenic activity of low molecular weight compounds based on IL-1α and IL-18 production by a murine and human keratinocyte cell line

“…The anatomical location of keratinocytes and their significant role in the development of allergic contact dermatitis justifies the use of these cells to evaluate sensitising potency. They not only represent the first target for irritants (DeLeo et al, 1987), but may also act to convert non-specific exogenous stimuli into the production of cytokines (Barker et al, 1991). In principle, a test system comprised of keratinocytes alone may not be useful in establishing allergenic potency as these cells lack antigen presenting capacity.…”

Assessment of Toxic Potential of Industrial Chemicals Using a Cultured Human Bioartificial Skin Model: Production of Interleukin 1α and Hydroxyeicosatetraenoic Acids

“…The ability of metabolites of arachidonic acid to mediate this type of localized vascular response in human skin suggests that the cell membrane may be a site of interaction for many cutaneous irritants [13]. Many of the lipoxygenase metabolites of arachidonic acid have been implicated in dose-related erythematous responses and are potent chemotactic agents [8].…”