Abstract:2004.-To determine whether prenatal surfactant storage was altered in a model of systemic arteriovenous fistula (SAVF) with pulmonary hypertension, a fistula was created between the internal jugular vein and the carotid artery in 120-day fetal lambs, and surfactant material was explored at 134 days. Total phospholipids (TPL) and disaturated phosphatidylcholine (DSPC) were increased in whole lung tissue. Phospholipid analysis of isolated lamellar body fraction evidenced a specific increase of surfactant pool si… Show more
“…Lipids were extracted from lung tissue homogenates (5-day-old pups) or from BAL fluid (7-dayold pups) using chloroform-methanol-water (1:2:0.8 vol/vol/vol). Individual phospholipids were separated by thin-layer chromatography, disaturated phosphatidylcholine (DSPC) was separated by osmium tetroxide and thin-layer chromatography, and then total phosphatidylcholine (PC), phosphatidylglycerol (PG), and DSPC spots revealed by iodine fixation were scrapped and mineralized, and phosphate was assayed as described previously (8).…”
Section: In Vivo Experimentsmentioning
confidence: 99%
“…Lung DNA was determined on the pellet of phospholipid extraction from lung tissue (5-day-old pups) by using the diphenylamine colorimetric method as described previously (8).…”
“…Lipids were extracted from lung tissue homogenates (5-day-old pups) or from BAL fluid (7-dayold pups) using chloroform-methanol-water (1:2:0.8 vol/vol/vol). Individual phospholipids were separated by thin-layer chromatography, disaturated phosphatidylcholine (DSPC) was separated by osmium tetroxide and thin-layer chromatography, and then total phosphatidylcholine (PC), phosphatidylglycerol (PG), and DSPC spots revealed by iodine fixation were scrapped and mineralized, and phosphate was assayed as described previously (8).…”
Section: In Vivo Experimentsmentioning
confidence: 99%
“…Lung DNA was determined on the pellet of phospholipid extraction from lung tissue (5-day-old pups) by using the diphenylamine colorimetric method as described previously (8).…”
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.