Surfactant protein B and A concentrations are increased in neonatal pneumonia

DaRonco, S.; Simonato, Manuela; Vedovelli, Luca; Baritussio, Aldo; Verlato, Giuseppe; Nobile, Stefano; Giorgetti, Chiara; Nespeca, Matteo; Carnielli, Virgilio P.; Cogo, Paola

doi:10.1038/pr.2015.123

Cited by 21 publications

(18 citation statements)

References 40 publications

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“…Increased levels of SP-B could also be associated with an increased cost of breathing, as suggested by the negative correlation between SP-B levels and dynamic compliance and by the finding that children ventilated for more than 48 h had a higher preoperative SP-B amount than those extubated within 48 h. Please note that this correlation was also confirmed in infants with stable hemodynamic conditions (VISo15) where the length of mechanical ventilation could be due to respiratory failure. Thus, SP-B could be a marker of lung injury, as previously reported in mice after endotoxin-mediated lung injury (30), or in neonatal pneumonia at the onset of respiratory failure (31).…”

Section: Discussionsupporting

confidence: 63%

Influence of the type of congenital heart defects on epithelial lining fluid composition in infants undergoing cardiac surgery with cardiopulmonary bypass

et al. 2018

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BackgroundIn children with congenital heart disease (CHD), altered pulmonary circulation compromises gas exchange. Moreover, pulmonary dysfunction is a complication of cardiac surgery with cardiopulmonary bypass (CPB). No data are available on the effect of different CHDs on lung injury. The aim of this study was to analyze epithelial lining fluid (ELF) surfactant composition in children with CHD.MethodsTracheal aspirates (TAs) from 72 CHD children (age 2.9 (0.4-5.7) months) were obtained before and after CPB. We measured ELF phospholipids, surfactant proteins A and B (SP-A, SP-B), albumin, and myeloperoxidase activity. TAs from 12 infants (age 1.0 (0.9-2.9) months) with normal heart/lung served as controls.ResultsHeart defects were transposition of great arteries (19), tetralogy of Fallot (TOF, 20), atrial/ventricular septal defect (ASD/VSD, 22), and hypoplastic left heart syndrome (11). Increased levels of ELF SP-B were found in all defects, increased myeloperoxidase activity in all except the TOF, and increased levels of ELF albumin and SP-A only in ASD/VSD patients. Postoperatively, ELF findings remained unchanged except for a further increase in myeloperoxidase activity.ConclusionELF composition has distinctive patterns in different CHD. We speculate that a better knowledge of the ELF biochemical changes may help to prevent respiratory complications.

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Section: Discussionsupporting

confidence: 63%

Influence of the type of congenital heart defects on epithelial lining fluid composition in infants undergoing cardiac surgery with cardiopulmonary bypass

et al. 2018

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“…We recently studied a group of 28 term newborns, 13 with pneumonia and 15 with no lung disease [63]. We measured SP-B, SP-A, DSPC and total phospholipid concentrations in tracheal aspirates at intubation and close to extubation.…”

Section: Neonatal Pneumoniamentioning

confidence: 99%

Neonatal Respiratory Diseases in the Newborn Infant: Novel Insights from Stable Isotope Tracer Studies

et al. 2016

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Respiratory distress syndrome is a common problem in preterm infants and the etiology is multifactorial. Lung underdevelopment, lung hypoplasia, abnormal lung water metabolism, inflammation, and pulmonary surfactant deficiency or disfunction play a variable role in the pathogenesis of respiratory distress syndrome. High-quality exogenous surfactant replacement studies and studies on surfactant metabolism are available; however, the contribution of surfactant deficiency, alteration or dysfunction in selected neonatal lung conditions is not fully understood. In this article, we describe a series of studies made by applying stable isotope tracers to the study of surfactant metabolism and lung water. In a first set of studies, which we call ‘endogenous studies', using stable isotope-labelled intravenous surfactant precursors, we showed the feasibility of measuring surfactant synthesis and kinetics in infants using several metabolic precursors including plasma glucose, plasma fatty acids and body water. In a second set of studies, named ‘exogenous studies', using stable isotope-labelled phosphatidylcholine tracer given endotracheally, we could estimate surfactant disaturated phosphatidylcholine pool size and half-life. Very recent studies are focusing on lung water and on the endogenous biosynthesis of the surfactant-specific proteins. Information obtained from these studies in infants will help to better tailor exogenous surfactant treatment in neonatal lung diseases.

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“…Dipalmitoyl lecithin is mainly distributed in the lung alveolar epithelium and is an ideal focus for the targeted release of nanoparticles. 15 Therefore, we attempted to use RNAi technology to silence the characteristics of specific gene expression by constructing PLGA-mediated TNF-α-siRNA alveolar epithelial cell (AEC)-targeting nanoparticles. These were administered to C57BL/6J mice with sepsis as a novel approach to gene- and drug-targeted therapy.…”

Section: Introductionmentioning

confidence: 99%

Tumor necrosis factor-α small interfering RNA alveolar epithelial cell-targeting nanoparticles reduce lung injury in C57BL/6J mice with sepsis

Qian

et al. 2021

J Int Med Res

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Background The role of tumor necrosis factor (TNF)-α small interfering (si)RNA alveolar epithelial cell (AEC)-targeting nanoparticles in lung injury is unclear. Methods Sixty C57BL/6J mice with sepsis were divided into normal, control, sham, 25 mg/kg, 50 mg/kg, and 100 mg/kg siRNA AEC-targeting nanoparticles groups (n = 10 per group). The wet:dry lung weight ratio, and hematoxylin and eosin staining, western blotting, and enzyme-linked immunosorbent assays for inflammatory factors were conducted to compare differences among groups. Results The wet:dry ratio was significantly lower in control and sham groups than other groups. TNF-α siRNA AEC-targeting nanoparticles significantly reduced the number of eosinophils, with significantly lower numbers in the 50 mg/kg group than in 25 mg/kg and 100 mg/kg groups. The nanoparticles also significantly reduced the expression of TNF-α, B-cell lymphoma-2, caspase 3, interleukin (IL)-1β, and IL-6, with TNF-α expression being significantly lower in the 50 mg/kg group than in 25 mg/kg and 100 mg/kg groups. Conclusion TNF-α siRNA AEC-targeting nanoparticles appear to be effective at improving lung injury-related sepsis, and 50 mg/kg may be a preferred dose option for administration.

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Surfactant protein B and A concentrations are increased in neonatal pneumonia

Cited by 21 publications

References 40 publications

Influence of the type of congenital heart defects on epithelial lining fluid composition in infants undergoing cardiac surgery with cardiopulmonary bypass

Influence of the type of congenital heart defects on epithelial lining fluid composition in infants undergoing cardiac surgery with cardiopulmonary bypass

Neonatal Respiratory Diseases in the Newborn Infant: Novel Insights from Stable Isotope Tracer Studies

Tumor necrosis factor-α small interfering RNA alveolar epithelial cell-targeting nanoparticles reduce lung injury in C57BL/6J mice with sepsis

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