Surfactant Protein D Gene Regulation

Protective role of the lung collectins surfactant protein A and surfactant protein D in airway inflammation

“…Sequence analysis of the SP-D gene promoter suggested potential regulatory roles for signal transducers and activators of transcription (STAT) , CCAAT/enhancer-binding protein (C/EBP), activator protein-1 (AP-1) , 78,87,88 TTF-1, and nuclear factor of activated T cells (NFAT) 71 binding sites (Fig 2, D ). The AP-1 complexes 87 and NFAT in cooperation with TTF-1 71 were shown to activate SP-D mRNA.…”

“…89–91 C/EBP (C/EBPα), C/EBPβ (inducible nuclear factor of IL-6 [NF–IL6]), and C/EBPδ (NF–IL6-β) 92 are expressed by lung epithelial cells, and each of them was shown to induce SP-D transcription. 88 Further, the mouse and rat promoter have an identical nonspecific STAT-binding site in a prominent location, proximal to the AP-1 site. This STAT-binding site has a nonspecific 5-base spacing motif 93 and could mediate the effects of IL-4/IL-13 and IL-6.…”

CCAAT/Enhancer-binding Protein β and NF-κB Mediate High Level Expression of Chemokine Genes CCL3 and CCL4 by Human Chondrocytes in Response to IL-1β*

“…The CCL3 and CCL4 promoter constructs, C/EBP␤ and IKK2 (IB kinase 2) expression vector, and pNF-B luciferase reporter were provided by the following. The human pGL2-CCL3(Ϫ1972/ϩ75) was from Dr. G. David Roodman (University of Pittsburgh) (26); human pGL3-CCL4(Ϫ1281/ ϩ12) was obtained from Dr. Sheau-Farn Yeh (National YangMing University, Taipei) (27); human IKK2 in the pCDNA3 vector and pNF-B luciferase reporter were from Dr. Yousef Abu-Amer (Washington University) (28); full-length human C/EBP in the pCDNA3 vector was from Dr. Erika Crouch (Washington University) (29). The empty expression vectors were made by excision of cDNAs from the corresponding C/EBP expression vectors (22).…”

“…In order to determine the relative roles of C/EBP␤ and NF-B in transcription of CCL3 and CCL4, we determined the location of binding sites on the genes and performed transfection studies with CCL3 (Ϫ1395) constructs (26), CCL4 (Ϫ1281) constructs (27), C/EBP␤ expression vector (29), and IKK2 expression vector (28). The CCL3 (Ϫ1395) and CCL4 (Ϫ1281) constructs contain several high probability candidate C/EBP␤ and NF-B binding sites as assessed by our computational data base (6,37,38) (Fig.…”

Nuclear Factor of Activated T Cells Regulates Transcription of the Surfactant Protein D Gene (Sftpd) via Direct Interaction with Thyroid Transcription Factor-1 in Lung Epithelial Cells

“…Retinoblastoma protein stimulated SFTPD activation by directly forming a complex with C/EBPs bound to the NF-IL-6 (C/ EBP-␤) consensus site in the SFTPD promoter. Cotransfection of C/EBP-␣, -␤, and -␦ cDNAs increased transcription of the human SFTPD gene in H441 cells (29).…”