Surfactant stimulation of growth in the nematode Caenorhabditis elegans

Continuous wave and simulated GSM exposure at 1.8 W/kg and 1.8 GHz do not induce hsp16‐1 heat‐shock gene expression in Caenorhabditis elegans

“…Cultures of PC72 worms were synchronised by filtration through a 5 m mesh filter [Mutwakil et al, 1997], and L1 larvae recovered from the filtrate were grown on 9 cm NGM agar plates [Sulston & Hodgkin, 1988] at 15C until they reached the L4/young adult stage. They were then washed off the plates with ice-cold K medium (53 mM NaCl, 32 mM KCl) [Williams & Dusenbery, 1990] and allowed to settle twice on ice for about 10 min to remove excess bacteria [Dawe et al, 2006].…”

A small temperature rise may contribute towards the apparent induction by microwaves of heat-shock gene expression in the nematodeCaenorhabditis Elegans

“…Worm culture, exposure and assay: PC72 worms were grown on NGM agar plates as described previously (Sulston and Hodgkin, 1988), and were synchronized by L1 filtration (Mutwakil et al, 1997) or by egg isolation using NaOCl (Sulston and Hodgkin, 1988). 1000 synchronous L4 larvae were placed in 1.0 ml of K medium into each well of a 24-well microplate kept on ice.…”

The toxicity of dithiocarbamate fungicides to soil nematodes, assessed using a stress-inducible transgenic strain ofCaenorhabditis elegans

“…PC72 worms were transferred onto NGM agar plates [37] preseeded with a lawn of E. coli (strain P90C), and cultured for 3-4 days at 15ЊC, then washed off with cold K medium (53 mM NaCl, 32 mM KCl [16]) and filtered through a 5 lm mesh [38]. L1 and L2 larvae were recovered from the filtrate by centrifugation (10,000 ‫ן‬ g for 1 min at 4(C [38]), resuspended in K medium, and aliquotted onto fresh bacterial lawns on NGM agar plates.…”