This work focused on revealing the removal mechanisms
of linear
alkylbenzenesulfonate (LAS) and nitrogen under low dissolved oxygen
(DO) conditions in a membrane biofilm reactor (MBfR) during greywater
treatment. High ammonium led to net accumulation of protein-enriched
extracellular polymeric substances (EPS). The biofilms with more protein-EPS
had faster kinetics for the removal of LAS, ammonium, and total nitrogen,
based on zero-order or first-order kinetics. High protein-EPS alleviated
LAS toxicity to microbial physiological functions and achieved stable
and efficient removal of LAS and nitrogen under low DO conditions.
With DO concentrations of 0.6 mg/L, all the biofilm samples achieved
efficient ammonia oxidation and LAS mineralization. When DO decreased
to 0.3 mg/L, enriching the biofilms with more ammonium led to increased
accumulation of LAS biodegradation-related genus and enzymes, nitrifiers
and ammonium monooxygenases (AMO), and denitrification-related heterotrophs
and reductases, all of which allowed the unaffected removal of LAS
and nitrogen under low DO conditions. Two metabolic LAS removal mechanisms
under low DO conditions were that LAS can be mineralized in the presence
of AMO and LAS oxidase, and LAS can be removed in the denitrification
process as the electron donor. Our results provided key information
for the practical application of O2-MBfR in low-energy-input
wastewater treatment.