Surfactin/iturin A interactions may explain the synergistic effect of surfactin on the biological properties of iturin A

Maget‐Dana, Régine; Thimon, Laurence; Peypoux, Françoise; Ptak, Marius

doi:10.1016/0300-9084(92)90002-v

Cited by 165 publications

(96 citation statements)

References 19 publications

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“…Lipopeptides, including surfactin, iturin and fengycin, were described previously as a major class of Bacillus peptide antibiotics, which have a great potential for biotechnological and biopharmaceutical applications (Peypoux et al, 1999). Iturins and fengycins display a strong antifungal activity, however, surfactins are not fungitoxic by themselves but retain some synergistic effect on the antifungal activity of Iturin A (Maget-Dana et al, 1992). Results found by Ongena et al (2005), strongly suggest that lipopeptides are responsible for most of the antifungal activity developed in vitro by B. subtilis toward multiple plant pathogens.…”

Section: Discussionmentioning

confidence: 99%

Antifungal activity and bioactive compounds produced by Bacillus mojavensis and Bacillus subtilis

Youcef-Ali¹,

Chaouche²,

Dehimat³

et al. 2014

Afr. J. Microbiol. Res.

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The soil bacteria, CWBI-B1567 and CWBI-B1568, isolated from arid regions and identified as Bacillus subtilis (accession number KC341751) and Bacillus mojavensis (accession number KC341749) respectively, were screened and evaluated for their antifungal activity against Candida albicans, one of the most important human fungal pathogens. In vitro assay with the antagonists and their cell-free culture supernatants on agar plates showed that the two Bacillus strains effectively inhibited growth of the yeast. The capacity of these Bacillus strains to produce the cell-wall degrading enzymes was further studied. B. subtilis and B. mojavensis are able to produce cellulase and protease, but not chitinase. Bioactive molecules were produced by B. subtilis CWBI-B1567 and B. mojavensis CWBI-B1568 in a liquid culture medium optimised for lipopeptide production. The antifungal activity was equally demonstrated by testing the resulting supernatants and lipopeptide-enriched extracts. The electro-spray mass spectrometry coupled to liquid chromatography (ESI-LC-MS) analysis showed that both the B. subtilis and the B. mojavensis produced surfactin and iturin. However the fengycin group was produced only by B. mojavensis. Through this study, we have demonstrated that B. subtilis and B. mojavensis have a strong antifungal activity especially against C. albicans. This growth inhibition is probably due to the production of cell-wall degrading enzymes and different families of lipopeptide including iturins, fengycins and surfactins.

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Section: Discussionmentioning

confidence: 99%

Antifungal activity and bioactive compounds produced by Bacillus mojavensis and Bacillus subtilis

Youcef-Ali¹,

Chaouche²,

Dehimat³

et al. 2014

Afr. J. Microbiol. Res.

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“…Biochemical experiments have shown that surfactin displays a synergistic effect on the biological properties of iturin A (25). Mycosubtilin belongs to the iturin family, and B. subtilis strain ATCC 6633 produces both mycosubtilin and surfactin (8).…”

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confidence: 99%

Novel Methods for Genetic Transformation of Natural Bacillus subtilis Isolates Used To Study the Regulation of the Mycosubtilin and Surfactin Synthetases

Duitman

Wyczawski

Boven

et al. 2007

Appl Environ Microbiol

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Natural isolates of Bacillus subtilis are often difficult to transform due to their low genetic competence levels. Here we describe two methods that stimulate natural transformation. The first method uses plasmid pGSP12, which expresses the competence transcription factor ComK and stimulates competence development about 100-fold. The second method stimulates Campbell-type recombination of DNA ligation mixtures in B. subtilis by the addition of polyethylene glycol. We employed these novel methods to study the regulation of the synthetases for the lipopeptide antibiotics mycosubtilin (myc) and surfactin (srfA) in B. subtilis strain ATCC 6633. By means of lacZ reporter fusions, it was shown that the expression of srfA is >100 times lower in strain ATCC 6633 than in the laboratory strain B. subtilis 168. Expression of the myc operon was highest in rich medium, whereas srfA expression reached maximal levels in minimal medium. Further genetic analyses showed that the srfA operon is mainly regulated by the response regulator ComA, while the myc operon is primarily regulated by the transition-state regulator AbrB. Although there is in vitro evidence for a synergistic activity of mycosubtilin and surfactin, the expression of both lipopeptide antibiotics is clearly not coordinated.

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“…The importance of bio-control and our search to find synergistic peptide combinations, such as iturin A and Srf Maget-Dana et al, 1992), motivated us to study the activity of GS and its producer A. migulanus on four B. subtilis strains, two Srf producers and two non-producers ( Bacterial strain identity and verification of peptide production.…”

Section: Introductionmentioning

confidence: 99%

Direct surfactin–gramicidin S antagonism supports detoxification in mixed producer cultures of Bacillus subtilis and Aneurinibacillus migulanus

et al. 2012

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Antibiotic production as a defence mechanism is a characteristic of a wide variety of organisms. In natural evolutionary adaptation, cellular events such as sporulation, biofilm formation and resistance to antibiotics enable some microorganisms to survive environmental and antibiotic stress conditions. The two antimicrobial cyclic peptides in this study, gramicidin S (GS) from Aneurinibacillus migulanus and the lipopeptide surfactin (Srf) from Bacillus subtilis, have been shown to affect both membrane and intercellular components of target organisms. Many functions, other than that of antimicrobial activity, have been assigned to Srf. We present evidence that an additional function may exist for Srf, namely that of a detoxifying agent that protects its producer from the lytic activity of GS. We observed that Srf producers were more resistant to GS and could be co-cultured with the GS producer. Furthermore, exogenous Srf antagonized the activity of GS against both Srf-producing and non-producing bacterial strains. A molecular interaction between the anionic Srf and the cationic GS was observed with circular dichroism and electrospray MS. Our results indicate that the formation of an inactive complex between GS and Srf supports resistance towards GS, with the anionic Srf forming a chemical barrier to protect its producer. This direct detoxification combined with the induction of protective stress responses in B. subtilis by Srf confers resistance toward GS from A. migulanus and allows survival in mixed cultures.

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Surfactin/iturin A interactions may explain the synergistic effect of surfactin on the biological properties of iturin A

Cited by 165 publications

References 19 publications

Antifungal activity and bioactive compounds produced by Bacillus mojavensis and Bacillus subtilis

Antifungal activity and bioactive compounds produced by Bacillus mojavensis and Bacillus subtilis

Novel Methods for Genetic Transformation of Natural Bacillus subtilis Isolates Used To Study the Regulation of the Mycosubtilin and Surfactin Synthetases

Direct surfactin–gramicidin S antagonism supports detoxification in mixed producer cultures of Bacillus subtilis and Aneurinibacillus migulanus

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