Surgical and prosthodontic residual ridge reconstruction with hydroxyapatite

Zeltser, Chassiel; Masella, Roger; Cholewa, Joe; Mercier, Paul

doi:10.1016/0022-3913(89)90179-0

Cited by 12 publications

(4 citation statements)

References 22 publications

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“…Bestirred by the great clinical uses of HAp as bone graft substitute or extender in the management of periodontal bony defects and cysts, as well as in the repair of maxillofacial/mandibular defects [33,34], we choose to examine the phase composition, crystal structure and morphology of the inorganic part of mandible bone and to develop HAp nanopowder with precise control of particle size, morphology, degree of crystallinity and chemical composition being further appropriate for mandible reparation. Thermal behaviour and carbonate content in biological and synthetic samples were analysed by the thermogravimetry/differential thermal analyses (TG/DTA) method.…”

Section: Introductionmentioning

confidence: 99%

Synthetical bone-like and biological hydroxyapatites: a comparative study of crystal structure and morphology

et al. 2011

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Phase composition, crystal structure and morphology of biological hydroxyapatite (BHAp) extracted from human mandible bone, and carbonated hydroxyapatite (CHAp), synthesized by the chemical precipitation method, were studied by x-ray powder diffraction (XRD), Fourier transform infrared (FTIR) and Raman (R) spectroscopy techniques, combined with transmission electron microscopy (TEM). Structural and microstructural parameters were determined through Rietveld refinement of recorded XRD data, performed using the FullProf computing program, and TEM. Microstructural analysis shows anisotropic extension along the [00l] crystallographic direction (i.e. elongated crystallites shape) of both investigated samples. The average crystallite sizes of 10 and 8 nm were estimated for BHAp and CHAp, respectively. The FTIR and R spectroscopy studies show that carbonate ions substitute both phosphate and hydroxyl ions in the crystal structure of BHAp as well as in CHAp, indicating that both of them are mixed AB-type of CHAp. The thermal behaviour and carbonate content were analysed using thermogravimetric and differential thermal analysis. The carbonate content of about 1 wt.% and phase transition, at near 790 °C, from HAp to β-tricalcium phosphate were determined in both samples. The quality of synthesized CHAp powder, particularly, the particle size distribution and uniformity of morphology, was analysed by a particle size analyser based on laser diffraction and field emission scanning electron microscopy, respectively. These data were used to discuss similarity between natural and synthetic CHAp. Good correlation between the unit cell parameters, average crystallite size, morphology, carbonate content and crystallographic positions of carbonate ions in natural and synthetic HAp samples was found.

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Section: Introductionmentioning

confidence: 99%

Synthetical bone-like and biological hydroxyapatites: a comparative study of crystal structure and morphology

et al. 2011

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“…In our current study, we have synthesized nanocomposites consisting of hydroxyapatite (HA) nanoparticles incorporated within cyclic acetal hydrogels for craniofacial tissue engineering applications. HA is a ceramic material which has been used for decades as an orthopedic or dental implant 4–7. HA has been considered a promising material for bone tissue engineering since its chemistry and structure resembles the mineral component of bone tissue 8–11.…”

Section: Introductionmentioning

confidence: 99%

“…HA is a ceramic material which has been used for decades as an orthopedic or dental implant. [4][5][6][7] HA has been considered a promising material for bone tissue engineering since its chemistry and structure resembles the mineral component of bone tissue. [8][9][10][11] Moreover, numerous studies have shown HA to be biocompatible, biodegradable, bioactive, and osteoconductive.…”

Section: Introductionmentioning

confidence: 99%

Characterization of cyclic acetal hydroxyapatite nanocomposites for craniofacial tissue engineering

Patel

Caccamese

et al. 2010

J Biomedical Materials Res

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Cyclic acetal hydrogels are a novel group of biomaterials which may facilitate osteogenic differentiation of encapsulated bone marrow stromal cells (BMSCs) because of their neutral degradation products. Here, we have incorporated hydroxyapatite nanoparticles within cyclic acetal hydrogels to create cyclic acetal nanocomposites for craniofacial tissue engineering applications. We hypothesized that inclusion of nanosized hydroxyapatite particles within cyclic acetal hydrogels would upregulate osteogenic signal expression of encapsulated BMSCs, due to enhanced cell adhesion, and therefore promote osteodifferentiation. Experimental nanocomposite groups consisted of lower (5 ng/mL) and higher (50 ng/mL) concentrations of nanoparticles. The nanocomposites were characterized by scanning electron microscopy, transmission electron microscopy, and energy dispersive spectroscopy. Swelling parameters of hydrogels in the presence of nanoparticles was studied. Osteoblastic differentiation was characterized by alkaline phosphatase (ALP) and osteocalcin (OC) expression, whereas endogenous osteogenic signal expression was characterized by morphogenetic protein-2 (BMP-2) expression. Finally, immunohistochemistry was performed to detect the presence of OC at the protein level. Results indicated that hydroxyapatite nanoparticles were uniformly distributed throughout the hydrogels and did not affect material properties of the gels. Viability of cells was not affected by nanoparticle concentration, and BMP-2 and OC mRNA expression was enhanced in the presence of nanoparticles. However, a difference in BMP-2, ALP, and OC mRNA expression was not noted between the lower and higher concentrations of nanoparticles. This work demonstrates that inclusion of hydroxyapatite nanoparticles within a cyclic acetal nanocomposite hydrogel may enhance BMSC differentiation by promoting endogenous osteogenic signal expression.

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“…In this current study, we synthesized cyclic acetal and hydroxyapatite (HA) composites (CAH) for craniofacial tissue‐engineering applications. HA is a ceramic material which has been used for decades for orthopaedic or dental implants (Castaldini and Cavallini, 1985; Haas et al , 2003; Kido and Saha, 1996; Zeltser et al , 1989). HA has shown potential as a material for bone tissue engineering because its chemistry and structure bears a resemblance to the mineral component of bone tissue (Friedman et al , 1998; Matsumoto et al , 2007; Wahl and Czernuszka, 2006; Yoshikawa and Myoui, 2005).…”

Section: Introductionmentioning

confidence: 99%

Cyclic acetal hydroxyapatite composites and endogenous osteogenic gene expression of rat marrow stromal cells

Patel

Dunn

Tostanoski

et al. 2009

J Tissue Eng Regen Med

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In this study, bone marrow stromal cells (BMSCs) were differentiated on cyclic acetal composites containing hydroxyapatite (HA) particles (110 or 550 nm). These composites were evaluated for their role in influencing osteogenic signalling by encapsulated BMSCs. While a number of factors exert influence on osteogenic signalling during the production of an osteogenic matrix, we hypothesize that HA particles may upregulate bone growth factor expression due to enhanced BMSC adhesion. To this end, fluorescence-activated cell sorting (FACS) analysis was performed for the evaluation of BMSC surface marker expression after culture on two-dimensional (2D) cyclic acetal/HA composites. Three-dimensional (3D) composites were then fabricated by incorporating 110 or 550 nm HA particles at 5, 10 and 50 ng/ml concentrations. Bone growth factor molecules (TGFbeta1, FGF-2 and PDGFa), bone biomarker molecules (ALP, OC, OPN and OCN) and extracellular matrix-related molecules (FN, MMP-13, Dmp1 and aggrecan) were selected for evaluation of osteogenic signalling mechanisms when in presence of these composites. FACS results at day 0 demonstrated that BMSCs were a heterogeneous population with a small percentage of cells staining positive for CD29, CD90 and CD51/61, while staining negative for CD34 and CD45. At day 3, a significant enrichment of cells staining strongly for CD29, CD90 and CD51/61 was achieved. Gene expression patterns for bone growth factors and extracellular matrix molecules were found to be largely dependent upon the size of HA particles. Bone marker molecules, except OCN, had unaltered expression patterns in response to the varied size of HA particles. Overall, the results indicate that larger-sized HA particles upregulate PDGF and these groups were also associated with the most significant increase in osteodifferentiation markers, particularly ALP. Our results suggest that endogenous signalling is dependent upon material properties. Furthermore, we propose that studying gene expression patterns induced by the surrounding biomaterials environment is a fundamental step in the creation of engineered tissues.

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Surgical and prosthodontic residual ridge reconstruction with hydroxyapatite

Cited by 12 publications

References 22 publications

Synthetical bone-like and biological hydroxyapatites: a comparative study of crystal structure and morphology

Synthetical bone-like and biological hydroxyapatites: a comparative study of crystal structure and morphology

Characterization of cyclic acetal hydroxyapatite nanocomposites for craniofacial tissue engineering

Cyclic acetal hydroxyapatite composites and endogenous osteogenic gene expression of rat marrow stromal cells

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