Surgical induced models of joint degeneration in the ovine stifle: Magnetic resonance imaging and histological assessment

Moya‐Angeler, Joaquin; Gonzalez-Nieto, Jimena; Monforte, Joaquin Sanchez; Altónaga, José R.; Vaquero, Javier; Forriol, Francisco

doi:10.1016/j.knee.2015.11.017

Cited by 7 publications

(10 citation statements)

References 41 publications

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“…Inter-rater reliability for MRI grading performed by two radiologists blinded to the injectate was interpreted as suggested in the literature[ 44 ] and agreement met or exceeded that reported in other image-based OA models[ 45 – 47 ]. Grading of cartilage damage at the lateral tibiofemoral and patellofemoral regions, bone marrow edema at the lateral tibiofemoral, medial tibiofemoral, and patellofemoral regions, and subchondral erosions at the lateral tibiofemoral and patellofemoral regions was “almost perfect” or “strong” ( Table 1 ).…”

Section: Resultsmentioning

confidence: 99%

“…We tailored the sequence acquisition parameters to produce high resolution, three-dimensional images of joint-specific structures, supporting reliability of grading between two blinded radiologists. Quantitation of MRI-pathology was achieved with a well-defined grading system adopted from clinical use that was shown to yield reproducible results with high degree of inter-rater agreement meeting or exceeding reference standards reported in other image-based OA models[ 45 – 47 ].…”

Section: Discussionmentioning

confidence: 99%

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Clinical magnetic resonance-enabled characterization of mono-iodoacetate-induced osteoarthritis in a large animal species

et al. 2018

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IntroductionOsteoarthritis (OA) is the most common form of arthritis. Medical and surgical treatments have yet to substantially diminish the global health and economic burden of OA. Due to recent advances in clinical imaging, including magnetic resonance imaging (MRI), a correlation has been established between structural joint damage and OA-related pain and disability. Existing preclinical animal models of OA are useful tools but each suffers specific roadblocks when translating structural MRI data to humans. Intraarticular injection of mono-iodoacetate (MIA) is a reliable, well-studied method to induce OA in small animals but joint size discrepancy precludes the use of clinical grade MRI to study structural disease. The porcine knee is suited for clinical MRI and demonstrates homology with humans. We set out to establish the first large animal model of MIA-induced knee OA in swine characterized by structural MRI.Materials and methodsYucatan swine (n = 27) underwent ultrasound-guided injection of knees with 1.2, 4, 12, or 40 mg MIA. MRI was performed at several time points over 12 weeks (n = 54 knees) and images were assessed according to a modified clinical grading scheme. Knees were harvested and graded up to 35 weeks after injection.ResultsMIA-injected knees (n = 25) but not control knees (n = 29) developed gross degeneration. A total of n = 6,000 MRI measurements were recorded by two radiologists. MRI revealed progressive cartilage damage, bone marrow edema, erosions, and effusions in MIA-injected knees. Lesion severity and progression was influenced by time, dose, and inter-individual variability.ConclusionsIntraarticular injection of MIA produced structural knee degradation that was reliably characterized using clinical MRI in swine. Destruction was progressive and, similar to human OA, lesion severity was heterogeneous between and within treatment groups.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Clinical magnetic resonance-enabled characterization of mono-iodoacetate-induced osteoarthritis in a large animal species

et al. 2018

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“…We adapted the sheep MRI Osteoarthritis Knee Score (MOAKS), a semi-quantitative score [ 23 ] that evaluates cartilage and bone marrow lesions (BML) in the knee. BML included cyst, osteophytes, synovitis and effusion.…”

Section: Methodsmentioning

confidence: 99%

Arthroscopic Tenotomy of the Long Head of the Biceps Tendon and Section of the Anterior Joint Capsule Produce Moderate Osteoarthritic Changes in an Experimental Sheep Model

Longo

Forriol

Candela

et al. 2021

IJERPH

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Osteoarthritis (OA) of the glenohumeral (GH) joint is a common cause of shoulder pain, resulting in considerable invalidity. Unfortunately, the study of its pathogenesis is challenging. Models of OA are necessary to identify specific targets for therapy and to be able to interfere with the development and evolution of OA. This study aims to assess the effect of an arthroscopic tenotomy of the long head of the biceps tendon (LHBT) and section of the anterior glenohumeral joint capsule on the ovine glenohumeral joint. In addition, the authors aim to validate and evaluate the reliability of a modified semi-quantitative MRI score to assess joint degeneration in a sheep’s shoulder. Eight skeletally mature sheep received an arthroscopic tenotomy of the LHBT and section of the anterior joint capsule and were euthanized four months after surgery. All animals tolerated the surgery well, and no complication was recorded for six weeks. Moderate degenerative changes to the ovine shoulder joint were found on MRI and histological evaluation. The arthroscopic tenotomy of the LHBT and the anterior glenohumeral joint capsule section caused moderate degenerative changes to the ovine shoulder joint.

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“…In small ruminants, the procedure of surgically transecting the cranial cruciate ligament (CCL), known as a cranial cruciate ligament desmotomy (CCLD) or anterior cruciate ligament transection, has been shown to induce osteoarthritis (OA) [1][2][3][4]. Sheep are often preferred for the CCLD model due to their ease of care, but also their articular anatomy, which closely resembles that of the human knee [5].…”

Section: Introductionmentioning

confidence: 99%

“…Unique in structure to large animals, the peroneus tertius (PT) muscle shares a proximal fused tendinous origin with the extensor digitorum longus and extensor digitorum medialis at the extensor fossa of the femur [6]. It is likely that the fused tendon of the PT, extensor digitorum longus, and extensor digitorum medialis contributes to stifle stability by preventing cranial subluxation of the tibia; however, this phenomenon and the biomechanics unique to these structures require further investigation and statistical validation [4].…”

Section: Introductionmentioning

confidence: 99%

Cranial Cruciate Ligament Desmotomies in Sheep Resulting in Peroneus Tertius Injury

Welsh

Collier

Clement

et al. 2021

Case Reports in Veterinary Medicine

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Surgical destabilization of the stifle joint via cranial cruciate ligament desmotomy (CCLD) is a routine procedure for the study of osteoarthritis (OA). Traditionally performed in rats, rabbits, cats, and dogs, CCLD in sheep provides an opportunity to study the pathology and treatment of joint instability in a species whose stifle better represents the equivalent human femorotibial joint. The surgical approaches for CCLD in sheep are variable and can result in inconsistent outcomes. Eight sheep underwent CCLD for use in a gene therapy study. We report this case in which six of the eight sheep were clinically diagnosed by pathognomonic signs and later confirmed by postmortem dissection, with injury of the peroneus tertius (PT) muscle. The PT plays a crucial role in the normal gait of large animals, including sheep. Injury to the PT results in failure of the reciprocal apparatus of the hind limb in which the hock can be extended during stifle flexion creating a varied gait and an indiscriminate increase in instability of the stifle and hock joints. Restricted movement postoperatively may provide decreased variability in surgical outcomes. Alternatively, increased stifle instability via CCLD coupled with PT transection or PT transection alone could potentially provide a superior model of stifle instability and OA development in sheep.

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Surgical induced models of joint degeneration in the ovine stifle: Magnetic resonance imaging and histological assessment

Cited by 7 publications

References 41 publications

Clinical magnetic resonance-enabled characterization of mono-iodoacetate-induced osteoarthritis in a large animal species

Clinical magnetic resonance-enabled characterization of mono-iodoacetate-induced osteoarthritis in a large animal species

Arthroscopic Tenotomy of the Long Head of the Biceps Tendon and Section of the Anterior Joint Capsule Produce Moderate Osteoarthritic Changes in an Experimental Sheep Model

Cranial Cruciate Ligament Desmotomies in Sheep Resulting in Peroneus Tertius Injury

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