Surgical preparation of rats and mice for intravital microscopic imaging of abdominal organs

Rhodes, George J.

doi:10.1016/j.ymeth.2017.07.003

Cited by 15 publications

(10 citation statements)

References 59 publications

(160 reference statements)

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“…The two forms of anesthesia used in our laboratory are the long acting thiobutabarbital derivative Inactin, used in terminal studies, and isoflurane used in serial imaging studies. For detailed information see the chapter by Rhodes in the journal Methods [14].…”

Section: Methodsmentioning

confidence: 99%

Fluorescent Imaging and Microscopy for Dynamic Processes in Rats

Sandoval

Molitoris

Palygin

2019

Methods in Molecular Biology

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The rat is a favored model organism to study physiological function in vivo. This is largely due to the fact it has been used for decades and is often more comparable to corresponding human conditions (both normal and pathologic) than mice. Although the development of genetic manipulations in rats have been slower than in mice, recent advances of new genomic editing tools allows for the generation of targeted global and specific cell type mutations in different rat strains. The rat is an ideal model for advancing imaging techniques like intravital multi-photon microscopy or IVMPM. Multi-photon excitation microscopy can be applied to visualize real-time physiologic events in multiple organs including the kidney. This imaging modality can generate 4-dimensional high resolution images that are inherently confocal due to the fact that the photon density needed to excite fluorescence only occurs at the objective focal plane; not above or below. Additionally, longer excitation wavelengths allow for deeper penetration into tissue, improved excitation, and are inherently less photo-toxic than shorter excitation wavelengths. Applying imaging tools to study physiology in rats has become a valuable scientific technique due to the relatively simple surgical procedures, improved quality of reagents, and reproducibility of established assays. In this chapter, the authors provide an example of the application of fluorescent techniques to study cardio-renal functions in rat models. Use of experimental procedures described here, together with multiple available genetic modified animal models, provide new prospective for the further application of multi-photon microscopy in basic and translational research.

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Section: Methodsmentioning

confidence: 99%

Fluorescent Imaging and Microscopy for Dynamic Processes in Rats

Sandoval

Molitoris

Palygin

2019

Methods in Molecular Biology

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“…However, this short-acting barbiturate derivative does not possess analgesic effects and is not recommended to be used alone. Also, mice’s response to thiobutabarbital is not consistent [ 102 ]. Accordingly, ketamine has been used in combination with thiobutabarbital [ 101 , 103 ].…”

Section: Maintenancementioning

confidence: 99%

Anesthesia and analgesia for common research models of adult mice

Ahmadi‐Noorbakhsh

Abbasi²,

Ghasemi³

et al. 2022

Lab Anim Res

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Anesthesia and analgesia are major components of many interventional studies on laboratory animals. However, various studies have shown improper reporting or use of anesthetics/analgesics in research proposals and published articles. In many cases, it seems “anesthesia” and “analgesia” are used interchangeably, while they are referring to two different concepts. Not only this is an unethical practice, but also it may be one of the reasons for the proven suboptimal quality of many animal researches. This is a widespread problem among investigations on various species of animals. However, it could be imagined that it may be more prevalent for the most common species of laboratory animals, such as the laboratory mice. In this review, proper anesthetic/analgesic methods for routine procedures on laboratory mice are discussed. We considered the available literature and critically reviewed their anesthetic/analgesic methods. Detailed dosing and pharmacological information for the relevant drugs are provided and some of the drugs’ side effects are discussed. This paper provides the necessary data for an informed choice of anesthetic/analgesic methods in some routine procedures on laboratory mice.

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“…Although intravital imaging is a powerful tool to study various biological processes in live organs and animals, several challenges need to be addressed, including anatomical constrains (i.e., organ accessibility), selection of appropriate techniques (e.g., type of incision and immobilisation of organ), and the use of inverted/upright microscopes (reviewed in [ 122 ]). Long-term imaging at a single-cell resolution in the cornea, skin, and hair follicles [ 123 , 124 ] does not require special preparation for obtaining optical access; however, small animals such as Drosophila melanogaster and zebrafish need to be anesthetised prior to imaging to permit the required surgical procedures to expose specific organs [ 106 , 109 ].…”

Section: Biological Models For Fluorescence Imaging; From the Monolayer Culture To The Whole Organmentioning

confidence: 99%

Fluorescence Microscopy—An Outline of Hardware, Biological Handling, and Fluorophore Considerations

Hickey

Ung

Bader

et al. 2021

Cells

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Fluorescence microscopy has become a critical tool for researchers to understand biological processes at the cellular level. Micrographs from fixed and live-cell imaging procedures feature in a plethora of scientific articles for the field of cell biology, but the complexities of fluorescence microscopy as an imaging tool can sometimes be overlooked or misunderstood. This review seeks to cover the three fundamental considerations when designing fluorescence microscopy experiments: (1) hardware availability; (2) amenability of biological models to fluorescence microscopy; and (3) suitability of imaging agents for intended applications. This review will help equip the reader to make judicious decisions when designing fluorescence microscopy experiments that deliver high-resolution and informative images for cell biology.

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Surgical preparation of rats and mice for intravital microscopic imaging of abdominal organs

Cited by 15 publications

References 59 publications

Fluorescent Imaging and Microscopy for Dynamic Processes in Rats

Fluorescent Imaging and Microscopy for Dynamic Processes in Rats

Anesthesia and analgesia for common research models of adult mice

Fluorescence Microscopy—An Outline of Hardware, Biological Handling, and Fluorophore Considerations

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