Nowadays, a number of pharmaceutical manufacturing technologies are closely related to the issues of research and the use of reference materials (RM). Fat-soluble vitamins are very significant in this group. This article discusses the possibilities of obtaining fat-soluble vitamins using low-waste resource-saving domestic technologies and using original purification methods; which allows us to assume the creation of domestic RM and relevant regulatory documents (RD) for their subsequent approval. The scientific novelty of the research lies in the development of an original domestic technology for obtaining the material and RM-fat-soluble vitamins. For the first time, low-waste technologies and original cleaning methods will be used for this purpose. For the first time, emphasis will be placed on the interaction of the obtained samples with related industries of production and science. Technological and regulatory documents confirming the effectiveness of the work will be created for the first time. Thus, for the first time will be created and approved from domestic production.