2022
DOI: 10.1021/acs.analchem.2c00987
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Surpassing the Background Barrier for Multidimensional Single-Molecule Localization Super-Resolution Imaging: A Case of Lysosome-Exclusively Turn-on Probe

Abstract: The background barrier restricts the dimensionality of live-cell single-molecule localization super-resolution imaging. Ideally, a probe exclusively turned on by its target, without any nonspecific fluorescence signals from off-target molecules, constitutes a practical solution to surpass this barrier. Yet, few such fluorophores have been developed. A lysosome with a unique acidic lumen was chosen as the target for demonstrating the concept advantage. A representative lyso-tracker Lyso-R (piperazine rhodamine)… Show more

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Cited by 12 publications
(7 citation statements)
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“…Rhodamine is the adequate fluorophore scaffold for super-resolution imaging credited to their robust photophysical properties and self-blinking possibilities from thermal spirocyclization equilibrium . In a landmark work, Urano et al first introduced a self-blinking rhodamine (HMSiR) for SMLM imaging through optimizing intramolecular nucleophilic fluorophores .…”
Section: Introductionmentioning
confidence: 99%
“…Rhodamine is the adequate fluorophore scaffold for super-resolution imaging credited to their robust photophysical properties and self-blinking possibilities from thermal spirocyclization equilibrium . In a landmark work, Urano et al first introduced a self-blinking rhodamine (HMSiR) for SMLM imaging through optimizing intramolecular nucleophilic fluorophores .…”
Section: Introductionmentioning
confidence: 99%
“…Rhodamine is the adequate fluorophore scaffold for super-resolution imaging credited to their robust photophysical properties, [13][14][15][16][17][18][19][20][21] and self-blinking possibilities from thermo spirocyclization equilibrium. 22 In a landmark work, Urano et al first introduced a self-blinking rhodamine (HMSiR) for SMLM imaging through optimizing of intramolecular nucleophilic fluorophores.…”
Section: Introductionmentioning
confidence: 99%
“…This technique requires unique molecular switches between dark and bright states and demands temporally and spatially isolated emission. , Thus, bright and photostable fluorophores with excellent blinking characteristics are particularly needed. Rhodamine derivatives are prominent candidates because they exist in proton-driven equilibria between the leuco spirocyclic form (ring-closed) and fluorescent zwitterionic (ring-opened) forms. Such self-blinking rhodamine can provide single-molecule signals with sufficient sparsity without the aid of ultraviolet light or redox additives and has become one of the popular strategies for developing SMLM fluorophores. …”
Section: Introductionmentioning
confidence: 99%